Simultaneous determination of sweeteners and preservatives in beverages by HPLC-DAD-ELSD
DOI:
https://doi.org/10.5219/1339Keywords:
sweeteners, preservatives, beverage, chromatography, DAD ELSD detectorsAbstract
People suffering from diabetes or being overweight must severely reduce their sugar use, often seeking food with sweeteners. Often, sugar is replaced by non-nutritious sweeteners in beverages, which also contain several other substances like vitamins, caffeine, amino acids, phenolic compounds and thus increasing the shelf life of the beverages is additionally treated with the addition of preservatives. As the concentration of additives in food (including beverages) is determined by the legislation in force, it is necessary to have an appropriate analytical method for food control. Since artificial sweeteners and preservatives are very different substances, they are determined separately using different HPLC methods. In this work HPLC method combining the advantages of specific (diode array detector, DAD) and universal (evaporative light scattering detector, ELSD) detector was validated and used for simultaneous determination of benzoic acid, sorbic acid, aspartame, acesulfame K, saccharin, sucralose and steviol glycosides in sugar-free beverages. The proposed analytical method showed good linearity, precision, and accuracy. Measured limits of detection (0.6 – 11.8 mg.dm-3 depending on the analyte) were sufficient to analyze 5-times diluted beverage samples. The validated method has been successfully used for the simultaneous analysis of artificial sweeteners and preservatives in beverage samples (energy drinks, ice teas, carbonated drinks). Except for steviol glycosides, the concentration of monitored substances in beverages did not exceed the maximum permitted concentrations given in the valid legislation.
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Bartnik, M., Norhammar, A., Rydén, L. 2007. Hyperglycaemia and cardiovascular disease. Journal of Internal Medicine, vol. 262, no. 2, p. 145-156. https://doi.org/10.1111/j.1365-2796.2007.01831.x DOI: https://doi.org/10.1111/j.1365-2796.2007.01831.x
Bassoli, A., Merlini, L. 2003. Sweeteners/Intensive. In Caballero, B. Encyclopedia of Food Sciences and Nutrition. Academic Press, p. 5688-5695. ISBN 978-0-12-227055-0. https://doi.org/10.1016/B0-12-227055-X/01172-X DOI: https://doi.org/10.1016/B0-12-227055-X/01172-X
de Queiroz Pane, D., Botelho Dias, C., Dillenburg Meinhart, A., Augusto Ballus, C., Teixeira Godoy, H. 2015. Evaluation of the sweetener content in diet/light/zero foods and drinks by HPLC-DAD. Journal of Food Science and Technology, vol. 52, no. 11, p. 6900-6913. https://doi.org/10.1007/s13197-015-1816-1 DOI: https://doi.org/10.1007/s13197-015-1816-1
Di Donna, L., Mazzotti, F., Santoro, I., Sindona, G. 2017. Tandem mass spectrometry: a convenient approach in the dosage of steviol glycosides in Stevia sweetened commercial food beverages. Journal of Mass Spectrometry, vol. 52, no. 5, p. 290-295. https://doi.org/10.1002/jms.3925 DOI: https://doi.org/10.1002/jms.3925
Dossi, N., Toniolo, R., Susmel, S., Pizzariello, A., Bontempelli, G. 2006. Simultaneous RP-LC determination of additives in soft drinks. Chromatographia, vol. 63, no. 11, p. 557-562. https://doi.org/10.1365/s10337-006-0793-y DOI: https://doi.org/10.1365/s10337-006-0793-y
Grembecka, M., Baran, P., Błażewicz, A., Fijałek, Z., Szefer, P. 2014. Simultaneous determination of aspartame, acesulfame-K, saccharin, citric acid and sodium benzoate in various food products using HPLC–CAD–UV/DAD. European Food Research and Technology, vol. 238, no. 3, p. 357-365. https://doi.org/10.1007/s00217-013-2111-x DOI: https://doi.org/10.1007/s00217-013-2111-x
Ha, M. S., Ha, S. D., Choi, S. H., Bae, D. H. 2013. Assessment of exposure of Korean consumers to acesulfame K and sucralose using a stepwise approach. International Journal of Food Sciences and Nutrition, vol. 64, no. 6, p. 715-723. https://doi.org/10.3109/09637486.2013.789491 DOI: https://doi.org/10.3109/09637486.2013.789491
Javanmardi, F., Nemati, M., Ansarin, M., Arefhosseini, S. R. 2015. Benzoic and sorbic acid in soft drink, milk, ketchup sauce and bread by dispersive liquid-liquid microextraction coupled with HPLC. Food Additives & Contaminants: Part B, vol. 8, no. 1, p. 32-39. https://doi.org/10.1080/19393210.2014.955534 DOI: https://doi.org/10.1080/19393210.2014.955534
Kamal, Y., O´Toole, S., Bernabé, E. 2019. Obesity and tooth wear among American adults: the role of sugar-sweetened acidic drinks. Clinical Oral Investigations, vol. 23, p. 1379-1385. https://doi.org/10.1007/s00784-019-03079-5 DOI: https://doi.org/10.1007/s00784-019-03079-5
Yongsun Lee, Y., Do, B., Lee, G., Lim, H. S., Yun, S. S., Kwon, H. 2017. Simultaneous determination of sodium saccharin, aspartame, acesulfame-K and sucralose in food consumed in Korea using high-performance liquid chromatography and evaporative light-scattering detection. Food Additives and Contaminants: Part A, vol. 34, no. 5, p. 666-677. https://doi.org/10.1080/19440049.2017.1284348 DOI: https://doi.org/10.1080/19440049.2017.1284348
Mortensen, A. 2006. Sweeteners permitted in the European Union: Safety aspects. Scandinavian Journal of Food & Nutrition, vol. 50, no. 3, p. 104-116. https://doi.org/10.1080/17482970600982719 DOI: https://doi.org/10.1080/17482970600982719
Shirivastava, A., Gupta, V. B. 2011. Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chronicles of Young Scientists, vol. 2, no 1, p. 21-25. https://doi.org/10.4103/2229-5186.79345 DOI: https://doi.org/10.4103/2229-5186.79345
Sik, B. 2012. Development and validation of a green high- performance liquid chromatographic method for the determination of some artificial sweeteners and caffeine in soft drinks. Food Analytical Methods, vol. 5, no. 6, p. 1443-1452. https://doi.org/10.1007/s12161-012-9385-7 DOI: https://doi.org/10.1007/s12161-012-9385-7
Silva, M. M., Lidon, F. C. 2016. Food preservatives-An overview on applications and side effects. Emirates Journal of Food and Agriculture, vol. 28, no. 6, p. 366-373. https://doi.org/10.9755/ejfa.2016-04-351 DOI: https://doi.org/10.9755/ejfa.2016-04-351
Wasik, A., McCourt, J., Buchgraber, M. 2007. Simultaneous determination of nine intense sweeteners in foodstuffs by high- performance liquid chromatography and evaporative light scattering detection - Development and single-laboratory validation. Journal of Chromatography A, vol. 1157, no. 1-2, p. 187-196. https://doi.org/10.1016/j.chroma.2007.04.068 DOI: https://doi.org/10.1016/j.chroma.2007.04.068
Yang, D. J., Chen, B. 2009. Simultaneous Determination of Nonnutritive Sweeteners in Foods by HPLC/ESI-MS. Journal of Agricultural and Food Chemistry, vol. 57, no. 8, p. 3022-3027. https://doi.org/10.1021/jf803988u DOI: https://doi.org/10.1021/jf803988u
Zygler, A., Wasik, A., Namieśnik, J. 2009. Analytical methodologies for determination of artificial sweeteners in foodstuffs. TrAC Trends in Analytical Chemistry, vol. 28, no. 9, p. 1082-1102. https://doi.org/10.1016/j.trac.2009.06.008 DOI: https://doi.org/10.1016/j.trac.2009.06.008
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