Chemical composition of chicken meat after application of humic acid and probiotic Lactobacillus fermentum


  • Peter Haščí­k Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Products, Trieda A. Hlinku 2, 949 76 Nitra
  • Henrieta Arpášová Department of Poultry and Small Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculure in Nitra, Trieda A. Hlinku 2, 949 76 Nitra
  • Adriana Pavelková Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Products, Trieda A. Hlinku 2, 949 76 Nitra
  • Marek Bobko Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Products, Trieda A. Hlinku 2, 949 76 Nitra
  • Juraj Čuboň Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Evaluation and Processing of Animal Products, Trieda A. Hlinku 2, 949 76 Nitra
  • Ondřej Bučko Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Department of Animal Husbandry, Tr. A. Hlinku 2, 949 76 Nitra



chemical composition, chicken meat, humic acids, dietery supplemation


The aim of the present study was analysed and evaluated chemical parameters of chicken breast and thigh muscles after addition of humic acids and probiotic into diet for broiler chicken. A total of 200 pcs Ross 308 broiler chickens were divided into 4 groups (n=50). The control group of chickens was fed with complete feed mixtures without any additives. Chickens in experiment groups were fed a diet containing: P1 (1% of humic acid), P2 (1% of humic acid and probiotic supplement Lactobacillus fermentum) and P3 were fed with complete feed mixture containing combination of starter feed mixture (1. - 21. day) with coccidiostaticum Diclazuril and growth feed mixture (21. - 35. day) containing Salinomycinum sodium. Besides, the groups were kept under the same conditions. Fattening period lasted for 42 days. Chicken meat was analyzed for content of water, crude protein, fat and cholesterol. Based on the results, we can state that the application of humic acids or the combination of Humac Natur with probiotic did not affect the chemical composition of the breast muscle. In the breast muscle, the protein content in the experimental group P3 with the coccidiostat (22.98 g.100 g-1) was reduced (p ≤0.05) compared to control group (23.42 g.100 g-1). In the case of thigh muscle was significantly higher content of fat and cholesterol (≤0.05) in chickens feeding with addition of Humac Natur (fat - 9.08 g.100g-1; cholesterol - 0.86 mg.100g-1) and similar results were recorded in experimental group with combination of Humac Natur and probiotic (fat - 9.15 g.100g-1; cholesterol - 0.86 mg.100g-1) compared to control group (fat - 7.15 g.100g-1; cholesterol - 0.70 mg.100g-1). From a general point of view, we can recommend the application of Humac Natur, respectively combination Humac Natur with probiotics in feeding of broiler chickens Ross 308.


Download data is not yet available.


Metrics Loading ...


Abdel-Latif, K. O., El-Husseiny, O. M., Abdallah, A. G. 2008. The Influence of Biological Feed Additives on Broiler Performance. International Journal of Poultry Science, vol. 7, no. 9, p. 862-871. DOI:

Ahmed, S. T., Islam, M. M., Bostami, A. B. M. R., Mun, H., Kim, Y., Yang. C. 2015. Meat composition, fatty acid profile and oxidative stability of meat from broilers supplemented with pomegranate (Punica granatum L.) by-products. Food Chemistry, vol. 188, p. 481-488. DOI:

Alkhalf, A., Alhaj, M., Al-Homidan, I. 2010. Influence of probiotic supplementation on blood parameters and growth performance in broiler chickens. Saudi Journal of Biological Sciences, vol. 17, no. 3, p. 219-225. DOI:

Angelovičová, M., Alfaig, E., Král, M., Tkáčová, J. 2013. The effect of the probiotics Bacillus Subtilis (PB6) on the selected indicators of the table eggs quality, fat and cholesterol. Potravinarstvo, vol. 7, no. 1, p. 80-84. DOI:

Bai, S. P., Wu, A. M., Sing, X. M., Lei, Y., Bai, J., Zhang, K. Y., Chio, J. S. 2013. Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens. Poultry Science, vol. 92, no. 3, p. 663-670. DOI:

Bajaj, B., Claes, I., Lebeer, S. 2015. Functional mechanisms of probiotics. Journal Of Microbiology, Biotechnology and Food Sciences, vol. 4, no. 4, p. 321-327. DOI:

Bernardeau. M., Vernoux, J. 2013. Overview of differences between microbial feed additives and probiotics for food regarding regulation, growth promotion effects and health properties and consequences for extrapolation of farm animal results to humans. Clinical Microbiology and Infection, vol. 19, no. 4, p. 321-330. DOI:

Blajman, J., Gaziano, C., Zbrun, M. V., Soto, L., Astesana, D., Berisvil, A., Scharpen, Signorini, M., Frizzo, L. 2015. In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens. Research in Veterinary Science, vol. 101, p. 50-56. DOI:

Bobko, M., Haščík, P., Bobková, A., Pavelková, A., Tkáčová, J., Trembecká, L. 2015. Lipid oxidation in chicken meat after application of bee pollen extract, propolis extract and probiotic in their diets. Potravinarstvo, vol. 9, no. 1, p. 342-346. DOI:

Brenes E., Roura A. 2010. Essential oils in poultry nutrition: Main effects and modes of action. Animal Feed Science and Technology. 2010. vol. 158, no. 1-2, p. 1-14. DOI:

Ceylan, N., Ciftci, I., Ilhan, Z. 2003. Effects of Some Alternative Feed Additives for Antibiotic Growth Promoters on Performance and Gut Microflora of Broilers. Turkish Journal of Veterinary and Animal Sciences, vol. 27, no. 3, p. 727-733.

Corzo, A., Fritts, C. A., Kidd, M. T., Kerr, B. J. 2005. Response of broiler chicks to essential and nonessential amino acid supplementation of low crude protein diets. Animal Feed Science and Technology, vol. 118, no. 3-4, p. 319-327. DOI:

Daneshmand, A., Sadeghi, G. H., Karimi, A., Vaziry, A., Ibrahim, S. A. 2015. Evaluating complementary effects of ethanol extract of propolis with the probiotic on growth performance, immune response and serum metabolites in male broiler chickens. Livestock Science, vol. 178, p. 195-201. DOI:

El-Husseiny, O. M., Abdallah, A. G., Abdel-Latif, K. O. 2008. The influence of biological feed additives on broiler performance. International Journal of Poultry Science, vol. 7, no. 9, p. 862-871. DOI:

Enberg, R. M., Hedemann. M. S., Leser, T. D., Jensen, B. B. 2000. Effect of Zinc Bacitracin and Salinomycin on Intestinal Microflora and Performance of Broilers. Poultry Science, vol. 79, no. 9, p. 1311-1319. DOI:

Eren, M., Deniz, G., Gezen, S. S., Türkmen, I. I. 2000. Broyler yemlerine kat lan humatlar n besiperformans, serum mineral konsantrasyonu ve kemik külü üzerine etkileri. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 47, no. 3, p. 255-263. DOI:

Giannenas, I., Papadopoulos, E., Tsalie, E., Triantafillou, E., Henikl, S., Teichmann, K., Tontis, D. 2012. Assessment of dietary supplementation with probiotics on performance, intestinal morphology and microflora of chickens infected with Eimeria tenella. Veterinary Parasitology, vol. 188, no. 1-2, p. 31-40. DOI:

Gomez, S., Angeles, M. L., Mojica, M. C., Jalukar, S. 2012. Combination of an enzymatically hydrolyzed yeast and yeast culture with a direct-fed microbial in the feeds of broiler chickens. Asian-Australasian Journal of Animal Sciences, vol. 25, no. 5, p. 665-673. DOI:

Gous, R. M. 2010. Nutritional limitations on growth and development in poultry. Livestock Science, vol. 130, no. 1-3, p. 25-32. DOI:

Haščík, P., Trembecká, L., Bobko, M., Kačániová, M., Čuboň, J., Kunová, S., Bučko, O. 2016. Effect of diet supplemented with propolis extract and probiotic additives on performance, carcass characteristics and meat composition of broiler chickens. Potravinarstvo, vol. 10, 2016, no. 1, p. 223-231. DOI:

Haščík, P., Trembecká, L., Bobko, M., Čuboň, J., Bučko, O., Tkáčová, J. 2015. Evaluation of Meat Quality after Application of Different Feed Additives in Diet of Broiler Chickens. Potravinarstvo, vol. 9, no. 1, p. 174-182. DOI:

Islam, K. M. S., Schuhmacher, A., Gropp, J. M. 2005. Humic acid substances in animal agriculture. Pakistan Journal of Nutrition, vol. 4, no. 3, p. 126-134. DOI:

Joo, S. T., Kim, G. D, Hwang, Y. H., Ryu, Y. C. 2013. Control of fresh meat quality through manipulation of muscle fiber characteristics. Meat Science, vol. 95, no. 4, p. 828-836. DOI:

Kabir, S. M. L., Rahman, M. M., Rahman, M. B., Rahman, M. M., Ahmed, S. U. 2004. The Dynamics of Probiotics on Growth Performance and Immune Response in Broilers. International Journal of Poultry Science, vol. 3, no. 5, p. 361-364. DOI:

Karaoglu, M., Aksu, M. I., Esenbuga, N., Kaya, M., Macit, M., Durdag, H. 2004. Effect of dietary probiotic on the pH and colour characteristics of carcasses, breast fillets and drumsticks of broilers. Animal Science, vol. 78, no. 2, p. 253-259. DOI:

Khaksefidi, A., Rahimi, S. H. 2005. Effect of Probiotic Inclusion in the Diet of Broiler Chickens on Performance, Feed Efficiency and Carcass Quality. Asian-Australasian Journal of Animal Sciences, vol. 18, no. 8, p. 1153-1156. DOI:

Kluth H., Rodehutscord M. 2006. The role of methodical aspects in the study of aminoacid subacute digestibility with poultry (Metodinių aspektų reikšmė amino rūgščių precekalinio virškinamumo tyrimuose su naminiais paukščiais). Veterinarija ir zootechnika, vol. 35, no. 57, p. 36-42.

Kocabağli, N., Alp, M., Acar, N, Kahraman, R. 2002. The effects of dietary humate supplemantation on broiler growth and carcass. Poultry Science, vol. 81, no. 2, p. 227-230. DOI:

Lee, K. W., Everts, H., Beynen, A. C. 2004. Essential oils in broiler nutrition. International Journal Poultry Science, vol. 3, no. 12, p. 738-752. DOI:

Lee, K. W., Everts, H., Kappert, H. J., Frehner, M., Losa, R., Beynen, A. C. 2003. Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science, vol. 44, no. 3, p. 450-457. DOI:

MacCarthy, P. 2001. The principles of humic substances. Soil Science, vol. 166, no. 11, p. 38-751. DOI:

Miezeliene, A., Alencikiene, G., Gruzauskas, R., Barstys, T. 2011. The effect of dietary selenium supplementation on meat quality of broiler chickens. Biotechnology, Agronomy, Society and Environment, vol. 15, no. S1, p. 61-69.

Mirnawati, Y. R., Marlida, Y. 2013. Effects of humic acid addition via drinking water on the performance of broilers fed diets containing fermented and non-fermented palm kernel cake. Archiva Zootechnica, vol. 16, no. 1, p. 41-53.

Mutus, R., Kocabagli, N., Alp, M., Acar, N., Eren, M., Gezen, S. S. 2006. The effect of dietary probiotic supplementation on tibial bone characteristics and strength in broilers. Poultry Science, vol. 85, no. 9, p. 1621-1625. DOI:

Nagaraju, R., Reddy, B. S. V., Gloridoss, R., Suresh, B. N., Ramesh, C. 2014. Effect of dietary supplementation of humic acids on performance of broilers. The Indian Journal of Animal Sciences, vol. 84, no. 4, p. 447-452.

Ondruška, Ľ., Chrastinová, Ľ., Rafay, J., Pospíšilová, D., Parkányi, V. 2012. Effect of humic substances and probiotics on growth performance and meat quality of rabbits. Potravinarstvo, vol. 6, no. 2, p. 39-41. DOI:

O'Sullivan, M. G. 2017. A Handbook for Sensory and Consumer-Driven New Product Development. Cambridge : Woodhead Publishing. 370 p. ISBN 978-0-08-100352-7.

Ozturk, E., Ocak, N., Coskun, I., Turhan, S., Erener, G. 2010. Effects of humic substances supplementation provided through drinking water on performance, carcass traits and meat quality of broilers. Journal of Animal Physiology and Animal Nutrition, vol. 94, no. 1, p. 78-85. DOI:

Ozturk, E., Ocak, N., Turan, A., Erener, G., Altop, A., Cankaya, S. 2011. Performance, carcass, gastrointestinal tract and meat quality traits, and selected blood parameters of broilers fed diets supplemented with humic substances. Journal of the Science of Food and Agriculture, vol. 92, no. 1, p. 59-65. DOI:

Rath, N. C., Huff, W. E., Huff, G. R. 2006. Effects of humic acid on broiler chickens. Poultry Science, 2006, vol. 85, no. 3, p. 410-414. DOI:

Shalmany, K. S., Shivazad, M. 2006. The Effect of Diet Propolis Supplementation on Ross Broiler Chicks Performance. International Journal of Poultry Science, vol. 5, no. 1, p. 84-88. DOI:

Reitznerová, A., Nagy, J., Marcinčák, S., Mačanga, J., Vaško, L., Vašková, J., Poláková, Z. 2016. The effect of humic acids on oxidative stability of frozen chicken. Bezpečnosť a kontrola potravín : Zborník prác z XIII. Vedeckej konferencie s medzinárodnou účasťou, Nitra : SPU, p. 182-185. ISBN 978-80-552-1481-8. (In Slovak)

Shim, Y. H., Ingale, S. L., Kim, J. S., Kim, K. H., Seo, D. K., Lee, S. C., Chae, B. J., Kwon, I. K. 2012. A multi-microbe probiotic formulation processed at low and high drying temperatures: effects on growth performance, nutrient retention and caecal microbiology of broilers. British Poultry Science, vol. 53, no. 4, p. 482-490. DOI:

Taklimi, S. M. S. M., Lotfollahian, H., Shahne, A. Z., Mirzaei, F., Alinejad, A. 2012. Study on efficacy of probiotic in broiler chickens diet. Agricultural Sciences, vol. 3, no. 1, p. 5-8. DOI:

Tateo, A., De Palo, P., Maggiolino, A., Centoducati, P. 2013. Post-thawing colour changes in meat of foals as affected by feeding level and post-thawing time. Archives Animal Breeding, vol. 56, no. 29, p. 293-302. DOI:

Uyeno, Y., Shigemori, S., & Shimosato, T. 2015. Effect of Probiotics/Prebiotics on Cattle Health and Productivity. Microbes and Environments, vol. 30, no. 2, p. 126-132. DOI:

Wang, Y., Sun, J., Zhong, H., Li, N., Xu, H., Zhu, Q., Liu, Y. 2017. Effect of probiotics on the meat flavour and gut microbiota of chicken. Scientific Reports. vol. 7, no. 1, p. 6400. DOI:

Yasar, S., Gokcimen, A., Altuntas, I., Yonden, Z., Petekkaya, E. 2002. Performance and ileal histomorphology of rats treated with humic acid preparations. Journal of Animal Physiology and Animal Nutrition, vol. 86, no. 7-8, p. 257-264. DOI:

Zhang, J. L., Xie, Q. M., Ji, J., Yang, W. H., Wu, Y. B., Li, C., Ma, J. Y., Bi, Y. Z. 2012. Different combinations of probiotics improve the production performance, egg quality, and immune response of layer hens. Poultry Science, vol. 91, no. 11, p. 2755-2760. DOI:

Zralý, Z., Písaříková, B. 2010. Effect of sodium humate on the content of trace elements in organs of weaned piglets. Acta Veterinaria Brno, vol. 79, no. 1, p. 73-79. DOI:




How to Cite

Haščí­k, P. ., Arpášová, H. ., Pavelková, A. ., Bobko, M. ., Čuboň, J. ., & Bučko, O. . (2018). Chemical composition of chicken meat after application of humic acid and probiotic Lactobacillus fermentum. Potravinarstvo Slovak Journal of Food Sciences, 12(1), 694–700.

Most read articles by the same author(s)

1 2 3 4 5 6 > >>