Evaluation of total polyphenol content and antioxidant capacity of different verity lupin seeds
Keywords:total polyphenols, antioxidant capacity, variety lupine seeds
Legumes, including lupins, beans, lentil and chickpea, are one of the most important crops in the world because of their nutritional quality. Lupin seeds have been used as human food and animal feed since ancient times. It was known that antioxidant photochemical in foods have many health benefits including prevention of various diseases associated with oxidative stress such as cancer, cardiovascular disease, neuro-degeneration and diabetes. Lupin grains are rich sources of complex carbohydrates, protein, vitamins and minerals. Antioxidants can be found naturally in foods. Total polyphenols content and antioxidant activity were measured in four varieties of lupin, namely in white lupin, blue lupin, yellow lupin and Mutabilis lupin species. A majority of antioxidants naturally present in foods occur in phenolic structures and especially in flavonoid structures. The content of the total polyphenols was determined by using the Folin-Ciocalteu reagent (FCR). Antioxidant activity was measured by using a compound DPPH˙ (2.2-diphenyl-1-picrylhydrazyl). In the present experiment according to the average contents of total polyphenols (TPC) in dry matter of lupin seeds there was the following line: L. Angustifolius (blue) lupin (696.212 mg GAE.100g-1) > L. Albus (white) lupin (614.13 mg GAE.100g-1) > L. Luteus (yellow) lupin (467.78 mg GAE.100g-1) > L. Mutabilis (pearl) lupin (367.36 mg GAE.100g-1). Based on the measured values of total antioxidant capacity (TAC) of lupin samples can be classified as follows: L. Albus (white) lupin (43.44%) >L. Angustifolius (blue) lupin (38.27%) >L. Luteus (yellow) lupin (22.29%) >L. Mutabilis (Pearl) lupin (20.80%). The relationship of antioxidant capacity with total polyphenolic was discussed. According to used statistical analyzes. Correlation between the phenolic contents and antioxidant capacity was significantly positive (r = 0.88). Our results confirmed that legumes can be a good source of bioactive compounds in the human nutrition. The main objective of the present work was to evaluated the content of total polyphenols and an antioxidant capacity of four Lupine species.
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