Production of enterotoxins of Staphylococcus spp. isolated from samples of sheep milk

Authors

  • František Zigo University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenskeho 73, 041 81 Kosice
  • Milan Vasiľ University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenskeho 73, 041 81 Kosice
  • Juraj Elečko University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenskeho 73, 041 81 Kosice
  • Martin Lapin University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenskeho 73, 041 81 Kosice
  • Zuzana Farkašova University of Veterinary Medicine and Pharmacy in Košice, Department of Animal Husbandry, Komenskeho 73, 041 81 Kosice

DOI:

https://doi.org/10.5219/361

Keywords:

sheep, hand milking, mastitis, pathogens, staphylococcal enterotoxins

Abstract

In our study was followed occurrence of mastitis in herd of 430 sheep of breed zoslachtena valaska with hand milking technology examined two times during one lactation season. Individual examination consisted from clinical examination of udder and microbiological examination of milk samples. By PCR was determined presence of genes coding production of enterotoxins, and by ELISA methods production individual types of enterotoxins. From individual forms of mastitis were frequently detected subacute (6.7%), subclinical (5.7%) and acute (2.9%). The coagulase-negative staphylococci (CNS) were identified in 102 (65.4%) from all 156 positive isolates. The CNS and S. aureus caused subacute (5.1%), subclinical (3.9%) and acute (2.4%) forms of mastitis. The most frequently isolated were S. epidermidis, followed by S. chromogenes and S. xylosus from ewes with subacute and subclinical mastitis. From acute and chronical forms of mastitis were  predominantly isolated S. aureusS. uberis and S. epidermidis. The production of staphylococcal enterotoxins (SE) - SEA, SEB, SEC, SED and the presence of genes sec (3), sea (2), seb (2) and sed (2) were determined in S. aureusS. epidermidisS. schleiferi and S. chromogenes, respectively. The results suggested on the high occurrence (12.4%) of subacute and subclinical forms. Confirmed production of enterotoxins and presence of genes coding their production present a risk for human health and decreased a quality of milk and products from sheep´s milk.

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References

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Published

2014-04-29

How to Cite

Zigo, F. ., Vasiľ, M. ., Elečko, J. ., Lapin, M. ., & Farkašova, Z. . (2014). Production of enterotoxins of Staphylococcus spp. isolated from samples of sheep milk. Potravinarstvo Slovak Journal of Food Sciences, 8(1), 92–96. https://doi.org/10.5219/361