The influence of different methods of decontamination of microbial biofilms formed on eggshells

Mykola Kukhtyn; Zhanna Sverhun; Yulia Horiuk; Volodymyr Salata; Svitlana Laiter-Moskaliuk; Mykhailo Mocherniuk; Larysa Kladnytska; Victor Horiuk

doi:10.5219/1981

Authors

Mykola Kukhtyn Ternopil Ivan Pului National Technical University, Faculty of Engineering of Machines, Structures and Technologies, Department of Food Biotechnology and Chemistry, Ruska, 56, 46001, Ternopil, Ukraine, Tel.: +380972392057 https://orcid.org/0000-0002-0195-0767
Zhanna Sverhun Podillia State University, Faculty of Veterinary Medicine and Technologies in Livestock, Department of Veterinary Obstetrics, Internal Pathology and Surgery, Schevchenko, 12, 32301, Kamianets-Podilskyi, Ukraine, Tel.: +380677343101
Yulia Horiuk Podillia State University, Faculty of Veterinary Medicine and Technologies in Livestock, Department of Veterinary Obstetrics, Internal Pathology and Surgery, Schevchenko, 12, 32301, Kamianets-Podilskyi, Ukraine, Tel.: +380976617964 https://orcid.org/0000-0002-7162-8992
Volodymyr Salata Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, Faculty of Veterinary Hygiene, Ecology and Law, Department of Veterinary-Sanitary Inspection, Pekarska, 50, 79010, Lviv, Ukraine, Tel.: +380677288933 https://orcid.org/0000-0002-7175-493X
Svitlana Laiter-Moskaliuk Podillia State University, Faculty of Veterinary Medicine and Technologies in Livestock, Department of Animal Hygiene and Veterinary Support of the Cynological Service of the National Police of Ukraine, Schevchenko, 12, 32301, Kamianets-Podilskyi, Ukraine, Tel.: +380974756864
Mykhailo Mocherniuk Podillia State University, Faculty of Veterinary Medicine and Technologies in Livestock, Department of Veterinary Obstetrics, Internal Pathology and Surgery, Schevchenko, 12, 32301, Kamianets-Podilskyi, Ukraine, Tel.: +380677954711
Larysa Kladnytska National University of Life and Environmental Sciences of Ukraine, FacultyVeterinary medicine, Department of Biochemistry and Physiology of Animals, Potechina Str., 16, 03127, Kyiv, Ukraine, Tel.: + 380631866233 https://orcid.org/0000-0002-9360-0587
Victor Horiuk Podillia State University, Faculty of Veterinary Medicine and Technologies in Livestock, Department of Veterinary Obstetrics, Internal Pathology and Surgery, Schevchenko, 12, 32301, Kamianets-Podilskyi, Ukraine, Tel.: +380679300964

DOI:

https://doi.org/10.5219/1981

Keywords:

chicken egg, disinfection, biofilm, eggshell microbiota, planktonic bacteria

Abstract

According to "food legislation" requirements, all eggs entering the production of egg products must be disinfected. Therefore, developing technologies for decontaminating chicken eggs before use for food purposes is a promising work direction in chicken egg production and storage. This research aimed to identify the microbiota of chicken eggs with varying degrees of shell contamination and determine the influence of different methods of decontaminating microbial biofilms formed on eggshells. It was set up that the quantitative content of microorganisms on the surface of chicken eggs ranged from 10³ CFU to 10⁶ CFU/ml of washing and depended on the contamination of the shell with droppings. Lactobacillus spp., Bacillus spp., Corynebacterium, Staphylococcus were among the genera of bacteria that prevailed on the clean chicken shell, which were isolated in 30-50% of cases, and gram-negative microbiota was practically absent. The constant release of gram-positive bacteria is noted on the contaminated eggshell, and the frequency of identification of gram-negative microbiota of the Enterobacteriaceae genus and non-fermenting genera Pseudomonas and Psychrobacter increases. That is, the microbial scape of the microbiota of the chicken shell depends on its cleanliness, and the presence of a dirty surface increases the frequency of allocation of the resident microflora of the gastrointestinal tract. It was found that the working solution of the disinfectant Vircon S destroyed planktonic bacteria applied to the eggshell in an average of 2 minutes of exposure, stabilised water ozone for 1 minute, gaseous ozone for 3 minutes, and the action of ultraviolet rays with a length of 253.7 nm for 25-30 min. At the same time, using these disinfection methods on bacteria formed in a biofilm on the eggshell did not cause a bactericidal action during this time. To significantly reduce bacteria in the biofilm using these methods, it is necessary to increase the exposure time of the biocide by 2-3 times. Therefore, the complex structure of the eggshell and the multi-layered matrix of biofilms provide better protection for bacteria against the influence of the investigated disinfection methods.

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