Acidification effects of starfruit (Averrhoa Bilimbi L.) on soy milk-based cottage cheese: A physicochemical and organoleptic assessment


  • I Ketut Budaraga Ekasakti University, Veteran Dalam no. 26B, 25115, Padang, West Sumatera, Indonesia, Tel.: +62812-83838-7468
  • Rera Aga Salihat Ekasakti University, Veteran Dalam no. 26B, 25115, Padang, West Sumatera, Indonesia, Tel.: +62812-6743-4430
  • Eddwina Aidila Fitria Ekasakti University, Veteran Dalam no. 26B, 25115, Padang, West Sumatera, Indonesia, Tel.: +62813-7427-1331



starfruit, cheese, storage, viability, acidification


Using organic acids from citrus plants such as lemon and lime as a coagulant in soft cheese has been widely practiced. However, Wuluh starfruit (Averrhoa Bilimbi L.) is rarely used, especially in making cottage cheese from soy milk. Wuluh starfruit, which has a distinctive taste and aroma and is not shared by other citrus fruits, has the potential to be utilized in making cottage cheese. This study aimed to determine and study the effect of using a natural coagulating agent, Wuluh starfruit juice, as a coagulant in making cottage cheese from soy milk. A completely Randomized Design with six levels of treatment and three replications was used as the research design. The treatments were variations in the addition of Wuluh starfruit juice, namely as follows: SKA0 = control, citric acid 0.4%, SKA1 = 10%, SKA2 = 20%, SKA3 = 30%, SKA4 = 40%, and SKA5 = 50%. Cottage cheese from treatment SKA3 was the most preferred by the panelists based on the organoleptic evaluation with taste value (6.16 ±0.94%), aroma value (6.16 ±0.94%), texture value (5.24 ±1.20%), colour value (5.32 ± 0.85%), and acceptability value (5.72 ±0.51). SKA3 treatment was also the most preferred on the physicochemical properties of yield (26.43 ±1.13%), moisture (62.21 ±0.20%), ash (1.70 ±0.03%), protein (16.36 ±0.25%), fat (18.28 ±0.19%), pH (3.66 ±0.02), vitamin C (224.36 ±0.01 mg/kg), antioxidant activity (69.44 ±1.60%) and salt (50.33 ±0.58 ppm).


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Li, Q., Xia, Y., Zhou, L., & Xie, J. (2013). Evaluation of the rheological, textural, microstructural and sensory properties of soy cheese spreads. In Food and Bioproducts Processing (Vol. 91, Issue 4, pp. 429–439). Elsevier BV. DOI:

Neeta J. Patil, Dr. M. I Talib, & Prof. Vishal R. Parate. (2015). Development of Soya Cheese. In International Journal of Engineering Research and (Vol. V4, Issue 11). ESRSA Publications Pvt. Ltd. DOI:

Arlene, A., Prima Kristijarti, A., & Ardelia, I. (2015). The Effects of the Types of Milk (Cow, Goat, Soya) and Enzymes (Rennet, Papain, Bromelain) Toward Cheddar Cheese Production. In Makara Journal of Technology (Vol. 19, Issue 1, p. 31). Universitas Indonesia, Directorate of Research and Public Service. DOI:

Josipović, R., Medverec Knežević, Z., Frece, J., Markov, K., Kazazić, S., & Mrvčić, J. (2015). Nutrition Quality and Microbiological Safety of Novel Cottage Cheese. In Food Technology and Biotechnology (Vol. 53). Faculty of Food Technology and Biotechnology - University of Zagreb. DOI:

Makki, G. M., Kozak, S. M., Jencarelli, K. G., & Alcaine, S. D. (2021). Evaluation of the efficacy of commercial protective cultures to inhibit mold and yeast in cottage cheese. In Journal of Dairy Science (Vol. 104, Issue 3, pp. 2709–2718). American Dairy Science Association. DOI:

Shao, T., Ma, X., Zhang, Y., Wu, R., Wang, X., Gu, R., & Chen, X. (2023). Physical and nutritional properties of Chinese Steamed Bun (mantou) made with fermented soy milk. In LWT (Vol. 183, p. 114849). Elsevier BV. DOI:

Mishra, B. K., Hati, S., Das, S., & Prajapati, J. B. (2019). Biofunctional Attributes and Storage Study of Soy Milk Fermented by Lactobacillus rhamnosus and Lactobacillus helveticus. In Food technology and biotechnology (Vol. 57, Issue 3, pp. 399–407). Faculty of Food Technology and Biotechnology - University of Zagreb. DOI:

Šertović, E., Sarić, Z., Barać, M., Barukčić, I., Kostić, A., & Božanić, R. (2019). Physical, Chemical, Microbiological and Sensory Characteristics of a Probiotic Beverage Produced from Different Mixtures of Cow’s Milk and Soy Beverage by Lactobacillus acidophilus La5 and Yoghurt Culture. In Food technology and biotechnology (Vol. 57, Issue 4, pp. 461–467). Faculty of Food Technology and Biotechnology - University of Zagreb. DOI:

Manuelian, C. L., Currò, S., Penasa, M., Cassandro, M., & De Marchi, M. (2017). Characterization of major and trace minerals, fatty acid composition, and cholesterol content of Protected Designation of Origin cheeses. In Journal of Dairy Science (Vol. 100, Issue 5, pp. 3384–3395). American Dairy Science Association. DOI:

Farkye, N. Y. (2004). Cheese technology. In International Journal of Dairy Technology (Vol. 57, Issues 2–3, pp. 91–98). Wiley. DOI:

Suryaningsih, S. (2016). Belimbing wuluh (Averrhoa bilimbi) sebagai sumber energi dalam sel galvani. In Jurnal Penelitian Fisika dan Aplikasinya (JPFA) (Vol. 6, Issue 1, p. 11). Universitas Negeri Surabaya. DOI:

Hanum, E. amelia R., Yulistiani, R., & Sarofa, U. (2022). Utilization of fruit extract as acidulant on physicochemical and sensory properties of cottage cheese with addition calcium chloride. In AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) (Vol. 6, Issue 2, pp. 15–21). SAFE Network. DOI:

Ali, M. B., Murtaza, M. S., Shahbaz, M., Sameen, A., Rafique, S., Arshad, R., Raza, N., Akbar, Z., Kausar, G., & Amjad, A. (2022). Functional, textural, physicochemical and sensorial evaluation of cottage cheese standardized with food grade coagulants. In Food Science and Technology (Vol. 42). FapUNIFESP (SciELO). DOI:

Konfo, T. R. C., Djouhou, F. M. C., Hounhouigan, M. H., Dahouenon-Ahoussi, E., Avlessi, F., & Sohounhloue, C. K. D. (2023). Recent advances in the use of digital technologies in agri-food processing: A short review. In Applied Food Research (Vol. 3, Issue 2, p. 100329). Elsevier BV. DOI:

Jiang, H., Yang, S., Tian, H., & Sun, B. (2023). Research progress in the use of liquid-liquid extraction for food flavour analysis. In Trends in Food Science & Technology (Vol. 132, pp. 138–149). Elsevier BV. DOI:

Koleva, I. I., van Beek, T. A., Linssen, J. P. H., Groot, A. de, & Evstatieva, L. N. (2002). Screening of Plant Extracts for Antioxidant Activity: a Comparative Study on Three Testing Methods. In Phytochemical Analysis (Vol. 13, Issue 1, pp. 8–17). Wiley. DOI:

Senduk, T. W., Montolalu, L. A. D. Y., & Dotulong, V. (2020). The rendement of boiled water extract of mature leaves of mangrove Sonneratia alba. In Jurnal Perikanan Dan Kelautan Tropis (Vol. 11, Issue 1, p. 9). Universitas Sam Ratulangi. DOI:

Kondyli, E., Katsiari, M. C., & Voutsinas, L. P. (2008). Chemical and sensory characteristics of Galotyri-type cheese made using different procedures. In Food Control (Vol. 19, Issue 3, pp. 301–307). Elsevier BV. DOI:

Adrianto, R., Wiraputra, D., Jyoti, M. D., & Andaningrum, A. Z. (2020). Soft Cheese Yield, Flavor, Taste, Overall Texture Made of Cow’s Milk Added Rennet and Lactid Acid Bacteria Yoghurt Biokul. In Jurnal Agritechno (pp. 120–126). Hasanuddin University, Faculty of Law. DOI:

Li, L., Chen, H., Lü, X., Gong, J., & Xiao, G. (2022). Effects of papain concentration, coagulation temperature, and coagulation time on the properties of model soft cheese during ripening. In LWT (Vol. 161, p. 113404). Elsevier BV. DOI:

O’Hare, T. J. (1993). Postharvest physiology and storage of carambola (starfruit) : a review. In Postharvest Biology and Technology (Vol. 2, Issue 4, pp. 257–267). Elsevier BV. DOI:

Komansilan, S., Rosyidi, D., Radiati, L. E., & Purwadi, P. (2019). Pengaruh variasi pH dengan penambahan enzim bromelin alami (Anannas comucus) terhadap sifat organoleptik keju cottage. In Jurnal Sains Peternakan (Vol. 7, Issue 1, pp. 54–61). University of Kanjuruhan Malang. DOI:

Wardhani, D. H., Jos, B., Abdullah, A., Suherman, S., & Cahyono, H. (2018). Effect of Coagulants in Curd forming in Cheese Making. In Jurnal Rekayasa Kimia & Lingkungan (Vol. 13, Issue 2, pp. 209–216). Chemical Engineering Department, Syiah Kuala University. DOI:

Banville, V., Power, N., Pouliot, Y., & Britten, M. (2015). Relationship between Baked‐Cheese Sensory Properties and Melted‐Cheese Physical Characteristics. In Journal of Texture Studies (Vol. 46, Issue 5, pp. 321–334). Wiley. DOI:

Mishra, B. B., Gautam, S., Chander, R., & Sharma, A. (2015). Characterization of nutritional, organoleptic and functional properties of intermediate moisture shelf stable ready-to-eat Carica papaya cubes. In Food Bioscience (Vol. 10, pp. 69–79). Elsevier BV. DOI:

Yasin, N. M. N., & Shalaby, S. M. (2013). Physiochemical and sensory properties of functional low fat cheesecake manufactured using cottage cheese. In Annals of Agricultural Sciences (Vol. 58, Issue 1, pp. 61–67). Elsevier BV. DOI:

Roikah, S., Rengga, W. D. P., Latifah, L., & Kusumastuti, E. (2016). Ekstraksi dan karakterisasi pektin dari belimbing wuluh (Averrhoa bilimbi,L). In Jurnal Bahan Alam Terbarukan (Vol. 5, Issue 1, pp. 29–36). Universitas Negeri Semarang. DOI:

Seebaluck-Sandoram, R., Lall, N., Fibrich, B., Blom van Staden, A., Saleem, H., & Mahomoodally, M. F. (2019). Antimicrobial, antioxidant and cytotoxic evaluation of two underutilised food plants: Averrhoa bilimbi L. (Oxalidaceae) and Phyllanthus acidus L. Skeels (Phyllanthaceae). In Biocatalysis and Agricultural Biotechnology (Vol. 18, p. 100998). Elsevier BV. DOI:

Mohd Suhaimi, N. I., Mat Ropi, A. A., & Shaharuddin, S. (2021). Safety and quality preservation of starfruit (Averrhoa carambola) at ambient shelf life using synergistic pectin-maltodextrin-sodium chloride edible coating. In Heliyon (Vol. 7, Issue 2, p. e06279). Elsevier BV. DOI:

Tabet, R., Mechai, A., Branes, Z., & Chenchouni, H. (2023). Effect of vegetable coagulant and lamb rennet on physicochemical composition, fatty acid profile and lipid quality indices of a traditional fresh cheese (Jben). In Biocatalysis and Agricultural Biotechnology (Vol. 47, p. 102609). Elsevier BV. DOI:

Kondyli, E., Pappa, E. C., Bosnea, L., Vlachou, A.-M., & Malamou, E. (2023). Chemical, textural and organoleptic characteristics of Greek semihard goat cheese made with different starter cultures during ripening and storage. In International Dairy Journal (Vol. 145, p. 105717). Elsevier BV. DOI:

Nina, D., Olga, K., Elena, V., Svetlana, K., Kermen, M., Arina, O., & Anandan, S. (2023). Influence of acoustic cavitation on physico-chemical, organoleptic indicators and microstructure of Adyghe cheese produced from cow and goat milk. In Ultrasonics Sonochemistry (Vol. 98, p. 106493). Elsevier BV. DOI:

Kondyli, E., Pappa, E. C., & Svarnas, C. (2016). Ripening changes of the chemical composition, proteolysis, volatile fraction and organoleptic characteristics of a white-brined goat milk cheese. In Small Ruminant Research (Vol. 145, pp. 1–6). Elsevier BV. DOI:

Busetta, G., Garofalo, G., Barbera, M., Di Trana, A., Claps, S., Lovallo, C., Franciosi, E., Gaglio, R., & Settanni, L. (2023). Metagenomic, microbiological, chemical and sensory profiling of Caciocavallo Podolico Lucano cheese. In Food Research International (Vol. 169, p. 112926). Elsevier BV. DOI:

Møller, K. K., Rattray, F. P., & Ardö, Y. (2013). Application of selected lactic acid bacteria and coagulant for improving the quality of low-salt Cheddar cheese: Chemical, microbiological and rheological evaluation. In International Dairy Journal (Vol. 33, Issue 2, pp. 163–174). Elsevier BV. DOI:

Bennato, F., Ianni, A., Bellocci, M., Grotta, L., Sacchetti, G., & Martino, G. (2023). Influence of dietary grape pomace supplementation on chemical and sensory properties of ewes’ cheese. In International Dairy Journal (Vol. 143, p. 105671). Elsevier BV. DOI:

Bhat, R., Ameran, S. B., Voon, H. C., Karim, A. A., & Tze, L. M. (2011). Quality attributes of starfruit (Averrhoa carambola L.) juice treated with ultraviolet radiation. In Food Chemistry (Vol. 127, Issue 2, pp. 641–644). Elsevier BV. DOI:

Moula Ali, A. M., Sant’Ana, A. S., & Bavisetty, S. C. B. (2022). Sustainable preservation of cheese: Advanced technologies, physicochemical properties and sensory attributes. In Trends in Food Science & Technology (Vol. 129, pp. 306–326). Elsevier BV. DOI:

MacLeod, G., & Ames, J. M. (1990). Volatile components of starfruit. In Phytochemistry (Vol. 29, Issue 1, pp. 165–172). Elsevier BV. DOI:

Saha, K. K., Rahman, A., Moniruzzaman, M., Syduzzaman, M., Uddin, M. Z., Rahman, M. M., Ali, M. A., al Riza, D. firmanda, & Oliver, M. M. H. (2023). Classification of starfruit maturity using smartphone-image and multivariate analysis. In Journal of Agriculture and Food Research (Vol. 11, p. 100473). Elsevier BV. DOI:

Diekman, C., Ryan, C. D., & Oliver, T. L. (2023). Misinformation and Disinformation in Food Science and Nutrition: Impact on Practice. In The Journal of Nutrition (Vol. 153, Issue 1, pp. 3–9). Elsevier BV. DOI:

El-Aidie, S. A. M., Mabrouk, A. M., Abd-Elgawad, A. R., & El- Garhi, H.-E. M. (2023). Physicochemical, textural and organoleptic properties of functional processed cheese manufactured from ultrafiltered milk. In Biocatalysis and Agricultural Biotechnology (Vol. 51, p. 102798). Elsevier BV. DOI:




How to Cite

I Ketut Budaraga, Rera Aga Salihat, & Eddwina Aidila Fitria. (2023). Acidification effects of starfruit (Averrhoa Bilimbi L.) on soy milk-based cottage cheese: A physicochemical and organoleptic assessment. Potravinarstvo Slovak Journal of Food Sciences, 17, 986–996.