Characteristics of probiotic glutinous rice tapai with the addition of Lactobacillus plantarum 1 RN2-53 and some natural dyes

Authors

  • Yusmarini Universitas Riau, Faculty of Agriculture, Department of Agricultural Technology, HR. Soebrantas KM. 12,5, 28293, Pekanbaru, Indonesia, Tel.: (+62)761-63272
  • Shanti Fitriani Universitas Riau, Faculty of Agriculture, Department of Agricultural Technology, HR. Soebrantas KM. 12,5, 28293, Pekanbaru, Indonesia, Tel.: (+62)761-63272 https://orcid.org/0000-0002-9693-7679
  • Vonny Setiaries Johan Universitas Riau, Faculty of Agriculture, Department of Agricultural Technology, HR. Soebrantas KM. 12,5, 28293, Pekanbaru, Indonesia, Tel.: (+62)761-63272
  • Netti Herawati Universitas Riau, Faculty of Agriculture, Department of Agricultural Technology, HR. Soebrantas KM. 12,5, 28293, Pekanbaru, Indonesia, Tel.: (+62)761-63272
  • Irhamdi Universitas Riau, Faculty of Agriculture, Department of Agricultural Technology, HR. Soebrantas KM. 12,5, 28293, Pekanbaru, Indonesia, Tel.: (+62)761-63272
  • Emma Riftyan Universitas Riau, Faculty of Agriculture, Department of Agricultural Technology, HR. Soebrantas KM. 12,5, 28293, Pekanbaru, Indonesia, Tel.: (+62)761-63272 https://orcid.org/0000-0001-7919-4319
  • Usman Pato Universitas Riau, Faculty of Agriculture, Department of Agricultural Technology, HR. Soebrantas KM. 12,5, 28293, Pekanbaru, Indonesia, Tel.: (+62)761-63272 https://orcid.org/0000-0001-7716-5282

DOI:

https://doi.org/10.5219/1898

Keywords:

Glutinous rice tapai, Tapai, probiotics, Lactobacillus plantarum 1 RN2-53, natural dyes

Abstract

Tapai is a traditional Indonesian food usually made from cassava and glutinous rice. The study aims to determine natural dyes that produce the highest quality probiotic glutinous rice tapai. The study was conducted experimentally using a complete randomized design consisting of four treatments, and each treatment was repeated four times. The treatment in this study was the soaking of glutinous rice into natural dye extracts, namely red dragon fruit extract, purple sweet potato extract, and suji leaf extract, with different concentrations, namely 10%, 15%, 20%, and 25%. The data obtained were statistically analyzed using variance analysis and continued with Duncan's new multiple-range test (DNMRT) at a level of 5%.  The results showed that natural dye extracts with different concentrations have a noticeable influence on total lactic acid, alcohol content, total lactic acid bacteria, antioxidant activity, and sensory characteristics of color, aroma, taste, and hardness.  The soaking treatment in red dragon fruit extract and purple sweet potato extract with a concentration of 20% resulted in glutinous rice tapai, preferred by panelists. Furthermore, the characteristic pH value of 3.35-3.41, total lactic acid 0.61-0.70%, alcohol content 0.33-0.42%, total lactic acid bacteria 9.11-9.40 CFU/mL, and antioxidant activity 167.35-102.51 ppm.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Khasanah, N., & Prima, R. W. (2014). Effect of fermentation time and addition of lactic acid bacteria Lactobacillus plantarum B1765 on the quality of cassava tapai product. In Unesa Journal of Chemistry (Vol. 3, Issue 1, pp. 78–84). Universitas Negeri Surabaya.

Yusmarini, J., V. S., Fitriani, S., Rahmayuni, Artanti, V. F., & Pato, U. (2019). Characteristics of probiotic tapai made by the addition of Lactobacillus plantarum 1. In International Journal of Agricultural Technology (Vol. 15, Issue 1, pp. 195–206). Association of Agricultural Technology in Southeast Asia (AATSEA).

Nataliani, M. M., Kosala, K., Fikriah, I., Isnuwardana, R., & Paramita, S. (2018). The Effect of Storage and Heating on Physical Stability and Antioxidant Activity of Natural Dye Solution from Dragon Fruit Flesh (Hylocereus costaricensis). In Jurnal Tumbuhan Obat Indonesia (Vol. 11, Issue 1, pp. 1–10). Badan Penelitian dan Pengembangan Kesehatan. https://doi.org/10.22435/toi.v11i1.8688.1-10 DOI: https://doi.org/10.22435/toi.v11i1.8688.1-10

Husna, N. el, Novita, M., & Rohaya, S. (2013). Anthocyanins Content and Antioxidant Activity of Fresh Purple Fleshed Sweet Potato and Selected Products. In Agritech (Vol. 33, Issue 3, pp. 296–302). Faculty of Agricultural Technology, Universitas Gadjah Mada. https://doi.org/10.22146/agritech.9551

Nugraheni, M. (2014). Natural Dyes: Sources and Their Application to Food and Health (1st ed.). Graha Ilmu.

Putri, D. C. L. A., Putra, I. N. K., & Suparthana, I. P. (2019). The Effect of Adding Red Dragon Fruit (Hylocereus Polyrhizus) Juice for The Characteristics of Yogurt Mixed Milk and Red Beans (Phaseolus Vulgaris). In Jurnal Ilmu Dan Teknologi Pangan (Vol. 8, Issue 1, pp. 8–17). Faculty of Agricultural Technology, Udayana University. https://doi.org/10.24843/itepa.2019.v08.i01.p02 DOI: https://doi.org/10.24843/itepa.2019.v08.i01.p02

Yusmarini, Y., Fitriani, S., Johan, V. S., Rahmayuni, R., & Artanti, V. F. (2020). Utilization of Lactobacillus plantarum 1 RN2-53 and plant-based natural dyes to manufacture probiotic tapai. In Jurnal Teknologi Dan Industri Pertanian Indonesia (Vol. 12, Issue 1, pp. 9–15). Agricultural Product Technology Department, Faculty of Agriculture, Universitas Syiah Kuala. https://doi.org/10.17969/jtipi.v12i1.15777 DOI: https://doi.org/10.17969/jtipi.v12i1.15777

Muchtadi, T. R., Sugiyono, & Ayustaningwarno, F. (2013). Food Science (4th ed.). CV. Alfabeta.

Cunniff, P., & Association of Official Analytical Chemists. (1995). Official methods of analysis of AOAC international (Association of Official Analytical Chemists, Ed.; 16th ed.). Association of Official Analytical Chemists.

Yulianti, C. H. (2014). Analysis of Different Alcohol Levels on Tape Rice, Black Glutinous Rice, and Cassava. In Jurnal Teknika (Vol. 6, Issue 1, pp. 531–536). Department of Informatics Engineering, Faculty of Engineering, Universitas Islam Lamongan.

Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. In LWT - Food Science and Technology (Vol. 28, Issue 1, pp 25–30). Elsevier. https://doi.org/10.1016/S0023-6438(95)80008-5 DOI: https://doi.org/10.1016/S0023-6438(95)80008-5

Fardiaz, S. (1992). Food Microbiology. Gramedia Pustaka Utama. Jakarta.

Yusmarini, Y., Pato, U., Johan, V. S., Ali, A., & Kusumaningrum, K. (2017). Characterization of Amylolytic Lactic Acid Bacteria from Sago Starch Processing Industry. In AgriTECH (Vol. 37, Issue 1, pp. 95–100). Faculty of Agricultural Technology, Universitas Gadjah Mada. https://doi.org/10.22146/agritech.17014 DOI: https://doi.org/10.22146/agritech.17014

Isnaini, H. N., Saputrayadi, A., & Sari, D. A. (2022). Quality of white glutinous rice tapai with the addition of dragon fruit as natural dye. In Food and Agro-Industry Journal (Vol. 3, Issue 2, pp. 199–211). Faculty of Agricultural Technology, Universitas Teknologi Sumbawa.

Dede, E. G., Nocianitri, K. A., & Darmayanti, L. P. T. (2018). The Effect of Time Addition Lactobacillus rhamnosus SKG 34 on Characteristics of Probiotic Fermented Sticky Riceduring Storage. Jurnal Ilmiah Teknologi Pertanian Agrotechno (Vol. 3, Issue 1, pp. 269-276). Faculty of Agricultural Technology, Universitas Udayana. https://doi.org/10.24843/JITPA.2018.v03.i01.p02 DOI: https://doi.org/10.24843/JITPA.2018.v03.i01.p02

Nurul, S. R., & Asmah, R. (2014). Variability in nutritional composition and phytochemical properties of red pitaya (Hylocereus polyrhizus) from Malaysia and Australia. In International Food Research Journal (Vol. 21, Issue 4, pp 1689–1697). Faculty of Food Science and Techology, Universiti Putra Malaysia.

Kakade, V., Jinger, D., Chavan, S., Nangare, D. D., Wakchaure, G. C., & Dinesh, D. (2020). Dragon Fruit: Wholesome and remunerative fruit crop for India. In Food and Scientific Reports (Vol. 1, Issue 12, pp. 44–48). Raiganj: Food and Scientific Reports.

Kurnianingsih, N., Ratnawati, R., Nazwar, T. A., Ali, M., & Fatchiyah, F. (2020). A Comparative Study on Nutritional Value of Purple Sweet Potatoes from West Java and Central Java, Indonesia. In Journal of Physics: Conference Series (Vol. 1665, Issue 1). IOP Publishing. https://doi.org/10.1088/1742-6596/1665/1/012011 DOI: https://doi.org/10.1088/1742-6596/1665/1/012011

Murtini, E. S., Adhamatika, A., & Putri, D. A. (2021). The effects of powdered suji leaves (Dracaena angustifolia (medik.) roxb.) on the pasting properties of wheat flour and characteristics of steamed bun. In IOP Conference Series: Earth and Environmental Science (Vol. 733, Issue 1, p. 012092). IOP Publishing. https://doi.org/10.1088/1755-1315/733/1/012092 DOI: https://doi.org/10.1088/1755-1315/733/1/012092

Kurniawan, T. B., Bintari, S.H., & Susanti, R. (2014). Interaction effects Tape and Bread Yeast on the Level of Bioethanol Cassava (Manihot utilissima, Pohl) Mukibat Varieties. In Jurnal Biosaintifika (Vol. 6, Issue 2, pp. 152–160). Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang. https://doi.org/10.15294/biosaintifika.v6i2.3783

Walker, G. M., & Stewart, G. G. (2016). Saccharomyces cerevisiae in the production of fermented beverages. In Beverages (Vol. 2, Issue 4, pp. 1–12). MDPI. https://doi.org/10.3390/beverages2040030 DOI: https://doi.org/10.3390/beverages2040030

Berlian, Z., Aini, F., & Ulandari, R. (2016). Evaluation of the alcohol content of glutinous rice white and cassava through fermentation with different doses of yeast. In Jurnal Biota (Vol 2, Issue 1, pp. 106–111). Department of Biology Faculty Saints and Technology, Universitas Islam Negeri Raden Fatah Palembang.

Marniza, M., Syafnil, S., & Safitri, S. (2020). Characterization of Tapai Ketan Hitam with Various Cooking Methods. In Jurnal Teknologi Agro-Industri (Vol. 7, Issue 2, pp. 112–120). Center for Research and Community Service, Politeknik Negeri Tanah Laut. https://doi.org/10.34128/jtai.v7i2.124 DOI: https://doi.org/10.34128/jtai.v7i2.124

Anisa, F. A., Bintoro, V. P., & Nurwantoro, N. (2017). Quality of Chemical and Organoleptic Tape from Fermented Taro Kimpul (Xanthosoma sagittifolium) with Various Concentrations of Yeast. In Jurnal Aplikasi Teknologi Pangan (Vol. 6, Issue 1, pp. 43–47). Faculty of Agriculture and Animal Husbandry, Universitas Diponegoro. https://doi.org/10.17728/jatp.207 DOI: https://doi.org/10.17728/jatp.207

Commission of the Indonesian Ulema Council. Commission of the Indonesian Ulema Council Number 10 Year 2018 Fatwa on Ethanol Content in Food and Beverage Product. Retrieved from https://www.halalmui.org/mui14/main/detail/memahami-fatwa-mui-tentang-kadar-etanol-pada-produk-makanan-dan-minuman.

Hasanah, U., Ratihwulan, H., & Nuraida, L. (2019). Sensory Profiles and Lactic Acid Bacteria Density of Tape Ketan and Tape Singkong in Bogor. In AgriTECH (Vol. 38, Issue 3, pp. 265-272). Faculty of Agricultural Technology, Universitas Gadjah Mada. https://doi.org/10.22146/agritech.30935 DOI: https://doi.org/10.22146/agritech.30935

Pato, U. (2003). Bile and Acid Tolerance of Lactic Acid Bacteria Isolated from Dadih and Their Antimutagenicity against Mutagenic Heated Tauco. In Animal Bioscience (Vol. 16, Issue 11, pp. 1680–1685). https://doi.org/10.5713/ajas.2003.1680 DOI: https://doi.org/10.5713/ajas.2003.1680

Usman, P., & Hosono, A. (1997). Antimutagenic activity of maillard reaction products againts mutagenic heated tauco. In Italian Journal of Food Science (Vol. 9, Issue. 4, pp. 267–276). Codon Publications.

Usman, P., & Hosono, A. (1999). Binding of cholesterol to the cells and peptidoglycan of Lactobacillus gasseri. Milchwissenschaft (Vol. 54, Issue 9, pp. 495–498) AVA-Verlag.

Harzallah, D., & Belhadj, H. (2013). Lactic Acid Bacteria as Probiotics: Characteristics, Selection Criteria and Role in Immunomodulation of Human GI Muccosal Barrier. In Lactic Acid Bacteria - R & D for Food, Health and Livestock Purposes. InTech. https://doi.org/10.5772/50732 DOI: https://doi.org/10.5772/50732

Molyneux, P. (2004). The Use of the Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity. In Songklanakarin Journal of Science and Technology (Vol. 26, Issue 2, pp. 211–219). INFORMS. https://doi.org/10.1287/isre.6.2.144 DOI: https://doi.org/10.1287/isre.6.2.144

Salim, M., Dharma, A., Mardiah, E., & Oktoriza, G. (2017). Influence of Antosianin and Antioxidant Influences Onprocess of Processing of Purlpe Sweet Potato. In Jurnal Zarah (Vol. 5, Issue 2, pp. 7–12). Faculty of Education Universitas Maritim Raja Ali Haji. https://doi.org/10.31629/zarah.v5i2.209 DOI: https://doi.org/10.31629/zarah.v5i2.209

González, E., Vaillant, F., & Pérez, A. (2012). In Vitro Cell-Mediated Antioxidant Protection of Human Erythrocytes by Some Common Tropical Fruits. In Journal of Nutrition & Food Sciences (Vol. 2, Issue 03, pp. 2–8). Longdom Publishing. https://doi.org/10.4172/2155-9600.1000139 DOI: https://doi.org/10.4172/2155-9600.1000139

Reshmi, S. K., Aravindhan, K. M., & Suganya Devi, P. (2012). The Effect of Light, Temperature, pH on Stability of Betacyanin Pigments in Basella alba Fruit. In Asian Journal of Pharmaceutical and Clinical Research (Vol. 5, Issue 4, pp. 107–110). Innovare Academic Sciences.

Hörtensteiner, S., & Kräutler, B. (2011). Chlorophyll breakdown in higher plants. In Biochimica et Biophysica Acta – Bioenergetics (Vol. 1807, Issue 8, pp. 977–988). Elsevier. https://doi.org/10.1016/j.bbabio.2010.12.007 DOI: https://doi.org/10.1016/j.bbabio.2010.12.007

Barus, T., & Wijaya, N. (2011). Dominant Microbiota and Their Role in Flavor of Cassava Tape. In Jurnal Biota (Vol. 16, Issue 2, pp. 354–361). Faculty of Biotechnology, Universitas Atma Jaya Yogyakarta. https://doi.org/10.24002/biota.v16i2.119 DOI: https://doi.org/10.24002/biota.v16i2.119

Widiyanti, N. L. M., & Sukarta, I. N. (2022). Various Comparisons of White Sticky Rice with Red Rice Toward the Volume of Brem Drink and Organoleptic Test Tape Foods. In JST (Jurnal Sains dan Teknologi) (Vol. 11, Issue 2, pp. 274–282). Universitas Pendidikan Ganesha. https://doi.org/10.23887/jstundiksha.v11i2.51055 DOI: https://doi.org/10.23887/jstundiksha.v11i2.51055

Agne, E. B. P., Hastuti, R., & Khabibi, K. (2010). Ekstraksi dan Uji Kestabilan Zat Warna Betasianin dari Kulit Buah Naga (Hylocereus polyrhizus) serta Aplikasinya sebagai Pewarna Alami Pangan. In Jurnal Kimia Sains dan Aplikasi (Vol. 13, Issue 2, pp. 51–56). Institute of Research and Community Services Diponegoro University (LPPM UNDIP). https://doi.org/10.14710/jksa.13.2.51-56 DOI: https://doi.org/10.14710/jksa.13.2.51-56

Asnawi, M., Sumarlan, S. H., & Bagus Hermanto, M. (2013). Characteristics Maturation Process of Cassava Tape (Manihot utilissima) Through the Use of Temperature Control. In Jurnal Bioproses Komoditas Tropis (Vol. 1, Issue 2, pp. 56–66) Department of Agricultural Engineering, Faculty of Agricultural Technology, Universitas Brawijaya.

Abdillah, J., Widyawati, N., & Suprihati. (2016). Pengaruh dosis ragi dan penambahan gula terhadap kualitas gizi dan organoleptik tape biji gandum. In Agric (Vol. 26, Issue 1, p. 75). Universitas Kristen Satya Wacana. https://doi.org/10.24246/agric.2014.v26.i1.p75-84 DOI: https://doi.org/10.24246/agric.2014.v26.i1.p75-84

Downloads

Published

2024-02-19

How to Cite

Yusmarini, Fitriani, S., Johan, V. S., Herawati, N., Irhamdi, Riftyan, E., & Pato, U. (2024). Characteristics of probiotic glutinous rice tapai with the addition of Lactobacillus plantarum 1 RN2-53 and some natural dyes. Potravinarstvo Slovak Journal of Food Sciences, 18, 109–121. https://doi.org/10.5219/1898