Characterization of Lactobacillus species proposed as probiotics
Keywords:Lactobacillus spp., acidic tolerance, bile tolerance, S-Layer
An isolated Lactobacillus from several various sources were identified depending on morphological, microscopically and biochemical tests in vitro analysis of probiotic properties that included: an ability to tolerate in different concentration of bile salt, survival in acidic conditions, their antimicrobial activity, and S-layer characterizations were carried out. It was noticed that isolates of Lactobacillus rhamnosus and L. delbrueckii have a broad activity of antimicrobial and found the isolate
L. rhamnosus represented with a survival percentage 6.9% at pH 4.5 and 5.1% at pH 2.0) also L. rhamnosus (5.7% at pH 4.5 and 4.9% at pH 2.0) tolerated acidic media, Lactobacillus spp. has antimicrobial activity against all gram-positive and negative tested isolates. 70 kDa of S-layer protein bands were detected with whole-cell SDS-PAGE analysis, and it's predominant in cells of isolates which grown in MRS broth anaerobically. It was noticed that the collected Lactobacillus isolates could be used as probiotic.
Altermann, E., Buck, B. L., Cano, R., Klaenhammer, T. R. 2004. Identification and phenotypic characterization of the cell-division CdpA. Gene, vol. 342, no. 1, p. 189-197. https://doi.org/10.1016/j.gene.2004.08.004
Åvall-Jääskeläinen, S., Palva, A. 2005. Lactobacillus surface layers and their applications. FEMS Microbiology Reviews, vol. 29, no. 3, p. 511-529. https://doi.org/10.1016/j.fmrre.2005.04.003
Baccigalupi, L., Di Donato, A., Parlato, M., Luongo, D., Carbone, V., Rossi, M., Ricca, E., De Felice, M. 2005. Small surface-associated factors mediate adhesion of a food isolated strain of Lactobacillus fermentum Caco-2 cells. Research in Microbiology, vol. 156, no. 7, p. 830-836. https://doi.org/10.1016/j.resmic.2005.05.001
Bauer, A. W., Kirby, W. M. M., Sherins, J. C., Turek, M.1996. Antibiotic Susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology, vol. 45, no. 4, p. 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
Bernardeau, M., Vernoux, J. P., Henri-Dubernet, S., Guégun, M. 2000. International Journal Food Microbiology, vol. 126, no. 3 p. 278-285. https://doi.org/10.1016/j.ijfoodmicro.2007.08.015
Boot, H. J., Kolen, C. P. A. M., van Noort, J. M., Pouwels, P. H. 1993. S-Layer Protein of Lactobacillus acidophilus ATCC 4356: purification, expression in Escherichia coli, and nucleotide sequence of the corresponding genet. The Journal of Bacteriology, vol. 175 no. 19 p. 6089-6096. https://doi.org/10.1128/jb.175.19.6089-6096.1993
Bradford, M. M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of dye-binding. Analytical Biochemistry, vol. 72, no. 1-2, p. 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
Caplice, E., Fitzgerald, G. F. 1999. Food fermentations: role of microorganisms in food production and preservation. International Journal of Food Microbiology, vol. 50, no. 1-2, p. 131-49. https://doi.org/10.1016/S0168-1605(99)00082-3
Casey, P. G., Casey, G. D., Gardiner, G. E., Tangney, M., Stanton, C., Ross, R. P., Hill, C., Fitzgerald, G. F. 2004. Isolation and Characterization of anti –Salmonella lactic acid bacteria from the porcine gastrointestinal tract. Letters in Applied Microbiology, vol. 39, no. 5, p. 431-438. https://doi.org/10.1111/j.1472-765X.2004.01603.x
De Roos, N. M., Katan, M. B. 2000.Effects of probiotic bacteria on diarrhoea, lipid metabolism, and carcinogenesis: a review of papers published between 1988 and 1998. The American Journal of Clinical Nutrition, vol. 71, no. 2, p. 405-411. https://doi.org/10.1093/ajcn/71.2.405
Duangjitcharoen, Y., Kantachote, D., Ongsakul, M., Poosaran, N., Chaiyasut, C. 2009.Potential use of probiotic Lactobacillus plantarum SS2 isolated from a fermented plant beverage: safety assessment and persistence in the murine gastrointestinal tract. World Journal of Microbiology and Biotechnology, vol. 25, p. 315-321. https://doi.org/10.1007/s11274-008-9894-0
Dunn, M. J. 1993. Gel Electrophoresis: proteins. Oxford. United Kingdom : Bios Scientific Publishers Limited, p. 176. ISBN 9781483193540.
Enan, G., Abdel-Shafi, S., Ouda, S., Negm, S.2013. Novel antibacterial activity of Lactococcus lactis subspecies lactis z11 isolated from zabady. International Journal of Biomedical Science, vol. 9, no. 3, p. 74-80.
Eschenbach, D. A., Davick, P. R., Williams, B. L., Klebanoff, S. J., Young-Smith, K., Critchlow, C. H., Holmes, K. 1989. In Prevalence of Hydrogen peroxide producing Lactobacillus species in normal women and women with bacterial vaginosis. Journal of Clinical Microbiology, vol. 27, no. 2, p. 251-256. https://doi.org/10.1128/JCM.27.2.251-256.1989
Falk, P. G., Hooper, L. V., Midtvedt, T., Gordon, J. I. 1998. Creating and maintaining the gastrointestinal ecosystem: what we know and need to know from Gnotobiology. Molecular Biology Review, vol. 62, no. 4, p. 1157-1170. https://doi.org/10.1128/MMBR.62.4.1157-1170.1998
Holzapfel, W. H., Harberer, P., Snel, J., Schillinger, U., Huisin’t Veld, J. H. J. 1998. Overview of gut flora and probiotics. International Journal of Food Microbiology, vol. 41, no. 2, p. 85-101. https://doi.org/10.1016/S0168-1605(98)00044-0
Hooper, L. V., Xu, J., Falk, P. G., Midtvedt, T., Jeffry, I. 1999. A molecular sensor that allows a gut commensal to control its nutrient foundation in a competitive ecosystem. Proceding of the NationalAcademy of Science of United State of America, vol. 96, no. 17, p. 9833-9838. https://doi.org/10.1073/pnas.96.17.9833
Kandler, O., Weiss, N.1986. Regular nonsporing Gram positive rods. In Sneath, P. H. A., Mair, N. S., Sharpe, M. E., Holt, J. H. Bergey’s Manual of Systematic bacteriology. Pennsylvánia, USA : Williams and Wilkins, p. 1208-1234. ISBN 0683078933.
Katla, A. K., Kruse, H., Johnsen, G., Herikstad, H. 2001. Antimicrobial susceptibility of starter culture bacteria used in Norwegian dairy products. InternationalJournal of Food Microbiology, vol. 67, no. 1-2, p. 147-152. https://doi.org/10.1016/S0168-1605(00)00522-5
Kim, P. I., Jung, M. Y., Chang, Y. H., Kim, S. J., Park, Y. H. 2007. Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract. Applied of Microbiology and Biotechnology, vol. 74, p. 1103-1111. https://doi.org/10.1007/s00253-006-0741-7
Kim, Y., Kim, S. H., Whang, K. Y., Kim, Y. J., Oh, S. 2008. Inhibition of Escherichia coli O157:H7 attachment by interactions between lactic acid bacteria and intestinal epithelial cells. Journal of Microbiology and Biotechnology, vol.18, p. 1278-1285.
Klein, G., Hallmann, C., Casas, I. A., Abad, J., Louwers, J., Reuter, G. 2000. Exclusion of Van A, Van B and Van C type glycopeptide resistance in strains of Lactobacillus reuteri and L. Rhamnosus used as probiotics by polymerase chain reaction and hybridization methods. Journal of Applied Microbiology, vol. 89, no. 5, p. 815-821. https://doi.org/10.1046/j.1365-2672.2000.01187.x
Kučerová, K., Chumchalová, J., Miková, K., Cupáková, Š., Karpišková, R., Ho, L. 2007. Screening of lactic acid bacteria for antimicrobial properties from mayonnaise-based products and raw materials. European Food Research and Technology, vol. 226, p. 265-272. https://doi.org/10.1007/s00217-006-0535-2
Laemmli, U. K. 1970.Cleavage of structural proteins during the assembly of the head of the bacteriophage. T4. Nature, vol. 227, p. 680-685. https://doi.org/10.1038/227680a0
Lu, R., Fasano, S., Madayiputhiya, N., Morin, N. P., Nataro, J., Fasano, A. 2009. Isolation, Identification, and Characterization of Small bioactive Peptides from Lactobacillus GG conditional media that exert both anti-Gram-negative and Gram-positive Bactericidal Activity. The Journal of Pediatric Gastroenterology and Nutrition, vol. 49, no.1, p.23-30. https://doi.org/10.1097/MPG.0b013e3181924d1e
Marteau, P., Rambaud, J. C. 2002. Probiotics and health: new facts and ideas. Current Opinion in Biotechnology, vol. 13, no. 5, p. 486-489.https://doi.org/10.1016/S0958-1669(02)00368-3
Masuda, K. 1992. Heterogeneity of S-layer proteins of Lactobacillus acidophilus strains. Microbiology and Immunology, vol. 36, no. 3, p. 297-301. https://doi.org/10.1111/j.1348-0421.1992.tb01666.x
Mesnage, S., Tosi-counter, E., Mock, M., Gounon, P., Fout, A. 1997. Molecular characterization of the Bacillus anthracis main S-layer component: evidence that it is the major Cell-associated antigen. Molecular Microbiology, vol. 23, p. 1147-1155. https://doi.org/10.1046/j.1365-2958.1997.2941659.x
Perdigon, G., Maldonado Galdeano, C., Valdez, J. C., Medici, M. 2002.Interaction of lactic acid bacteria with the gut immune system. European Journal of Clinical Nutrition, vol, 56, no. 4 p. S21-S26. https://doi.org/10.1038/sj.ejcn.1601658
Perdigon, G., Mdina, M., Vintiñi, E., Valdez, J. C. 2000. Intestinal pathway of internalization of lactic acid bacteria and gut mucosal immunostimulation. International Journal of Immunopathology and Pharmacology, vol. 13, no. 3, p. 141-150.
Perelmuter, K., Fraga, M., Zunino, P. 2008.In vitro activity of potential probiotic Lactobacillus murinus isolated from the dog. Journal of Applied Microbiology, vol. 104, no. 6, p. 1718-1725. https://doi.org/10.1111/j.1365-2672.2007.03702.x
Rodes, L., Khan, A., Paul, A., Coussa-Charley, M., Marinescu, D., Tomaro-Duchesneau, C., Shao, W., Kahouli, I., Prakash, S. 2013. Effect of Probiotics Lactobacillus and Bifidobacterium on gut-derived lipopolysaccharides and inflammatory cytokines: an in vitro study using a human colonic microbiota model. Journal of Microbiology and Biotechnology, vol. 23, no. 4 p. 518-26. https://doi.org/10.4014/jmb.1205.05018
Samells, J., Taakalidon, E., Metaxopoulos, J., Kalantzopoulus, G. 1995. Differentiation of Lactobacillus sake and Lact. curvatus isolated from naturally fermented Greek dry Salami by SDS-PAGE of whole-cell proteins. Journal of Applied Bacteriology, vol. 78, no. 2, p. 157-163. https://doi.org/10.1111/j.1365-2672.1995.tb02836.x
Sanders, M. E., Klaenhammer, T. R. 2001. Invited Review: The Scientific basis of Lactobacillus Acidophilus NCFM functionality as a probiotic. Journal of Dairy Science, vol. 84, no. 2, p. 319-331. https://doi.org/10.3168/jds.S0022-0302(01)74481-5
Sára, M., Sleyrtr, U. B. 2000. S-layer proteins. Journal of Bacteriology, vol. 182, no. 4, p. 859-868. https://doi.org/10.1128/JB.182.4.859-868.2000
Sghir, A., Gramet, G., Suau, A., Rochet, V., Pochert, P., Dore, J. 2000.Quantification of bacterial groups within human facial flora by oligonucleotide probe hybridization. Applied and Environmental Microbiology, vol. 66, no. 5, p. 2263-2266. https://doi.org/10.1128/AEM.66.5.2263-2266.2000
Smit, E., Oling, F., Demel, R., Martiez, B., Pouwels, P. H. 2001. The S-layer protein of Lactobacillus acidophilus ATCC 4356: Identification and characterization of domains responsible for S-protein assembly and cell wall binding. Journal of Molecular Biology, vol, 305. no. 2, p. 245-257. https://doi.org/10.1006/jmbi.2000.4258
Suvarna, V. C., Boby, V. U. 2005. Probiotics in human health: a current assessment. Current Science, vol. 88, no. 11, p. 1744-1748.
Takeoka, A., Takumi, K., Koga, T., Kawata, T. 1991. Purification and characterization of S-layer proteins from Clostridium difficile GAI 0714. Journal of Genetic. Microbiology, vol. 137 no. 2, p. 261- 267. https://doi.org/10.1099/00221287-137-2-261
Wayne, P. 2015. Performance standards for antimicrobial susceptibility testing; twenty-fifty informational supplement. CLSI DocumentM100-S25. Clinical and Laboratory Standards Institute.
Yasui, T., Yoda, K., Kamiya, T. 1995. Analysis of S-layer proteins of Lactobacillus brevis. FEMS MicrobiologyLetters, vol. 133, no. 1-2, p. 181-186. https://doi.org/10.1111/j.1574-6968.1995.tb07881.x
How to Cite
Copyright (c) 2021 Potravinarstvo Slovak Journal of Food Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).