Analysis of pork adulteration in the corned products using FTIR associated with chemometrics analysis


  • Any Guntarti Universitas Ahmad Dahlan, Faculty of Pharmacy, Yogyakarta 55164, Indonesia, Tel.: (0274) 563515
  • Mustofa Ahda Universitas Ahmad Dahlan, Faculty of Pharmacy, Yogyakarta 55164, Indonesia, Tel.: (0274) 563515
  • Aprilia Kusbandari Universitas Ahmad Dahlan, Faculty of Pharmacy, Yogyakarta 55164, Indonesia, Tel.: (0274) 563515
  • Faradita Natalie Universitas Ahmad Dahlan, Faculty of Pharmacy, Yogyakarta 55164, Indonesia, Tel.: (0274) 563515



beef fat, FTIR spectra, lard, PCA, PLS


Meat-based foods such as beef corned became one of the targets of counterfeiting with pork because relatively cheaper. This becomes a serious problem for Muslims, especially in Indonesia. One method that can be used to detect fat was Fourier transform infrared (FTIR) spectrophotometry. The purpose of this study was to quantitatively analyze and a group of corned beef and corned pork using FTIR spectrophotometry combined with chemometrics. Reference samples corned pork-beef made of 7 various concentration (0%, 25%, 35%, 50%, 65%, 75%, 100%) and 6 product samples purchased in the Umbulharjo, Yogyakarta. Extraction was carried out by the soxhlet apparatus using n-hexane technical solvent for 4 – 5 hours at 69 – 70 °C. Fat analyzed using FTIR spectrophotometry for generating infrared spectral data then processed with Partial least square (PLS) chemometrics for quantitative analysis and Principal component analysis (PCA) for grouping. Results of quantitative analysis chemometrics PLS, selected areas fingerprints for analysis corned pork-beef was 1180 – 730 cm-1 with R2 0.9833; RMSEC 2.06%; RMSEP 1.65% and RMSECV 2.22%. The results of PCA showed groupings in different quadrants between corned pork 100% and corned beef 100%. Results showed that FTIR spectrophotometry combined with chemometrics can be used for quantitative analysis and grouping of pork corned and beef corned on the market but it can not identify pork in corned after choking process.


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Ahda, M., Guntari, A., Kusbandari, A. 2016. Application of HPLC (High Pressure Liquid Chromatography) for Analysis of Lard in the Meatball Product Combined with PCA (Principal Component Analysis). Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 6, p. 120-123. DOI:

Ahda, M., Guntari A, Kusbandari, A., Melianto, Y. 2020. Authenticity Analysis of Beef Meatball Adulteration with Wild Boar using FTIR Spectroscopy Combined with Chemometrics. Journal Microbiology, Biotechnology, and Food Science., vol. 9, no. 5, p. 937-940. DOI:

Ahda, M., Safitri, A. 2016. Development of Lard Detection in Crude Palm Oils (CPO) using FTIR Combined with Chemometrics Analysis. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 12, p. 307-309.

Bhaskar, R., Bhaskar, R., Sagar, M. K., Saini, V., Bhat, K. 2012. Simultaneous Determination of Ve-rapamil Hydrochloride and Gliclazide in Synthetic Binary Mixture and Combined Tablet Preparation by Chemometric-As-sisted Spectroscopy. Journal of Analyti-cal Sciences, Methods and Instrumentation, vol. 2, p. 161-166. DOI:

Belitz, H. D., Grosch, W., Schieberle, P. 2009. Food Chemistry 4th revised and extended Edition, Berlin, Germany : Springer Verlag Heidelberg. p. 640-651.

Che Man, Y. B., Syahariza, Z. A., Rohman, A. 2010. Analysis of Fats and Oils, in Fourier Transform Infrared Spectroscopy. In Rees, O. J. Fourier Transform Infrared (FTIR) Spectroscopy: Developments, Tehniques and Applications. UK : Nova Science Pub Inc. ISBN: 978-1616688356.

Che Man, Y. B., Rohman, A. 2011. Differentiation of Lard from Other Edible Fats and Oils by Means of Fourier Transform Infrared Spectroscopy and Chemometrics. Journal of American Oil Chemist' Society, vol. 88, no. 2, p. 187-192. DOI:

Che Man, Y. B., Marina, A. M., Rohman, A., Al-Kahtani, H. A., Norazura, O. 2013. A Fourier Transform Infrared Spectroscopy Method for Analysis of Palm Oil Adulterated with Lard in Pre-Fried French fries. International Journal of Food Properties. vol. 17, no. 2, p. 354-362. DOI:

El-Gindy, A., Emara, S., Mostafa, A. 2006. Application and Validation of Chemometrics-Assited Spectrophotometry and Liquid Chromatography for The Simultaneous Determination of Six-Component Pharmaceuticals. Journal of Pharmaceutical and Biomedical Analysis, vol. 41, no. 2, p. 421-430. DOI:

Gamperline, P. 2006. Practical Guide to Chemom-etrics, 2nd Ed. Boca Raton, FL, USA : Taylor and Francis Group. p. 115, ISBN-13: 978-1-57444-783-5

Garcia, M. J. L. 2012. Characterization and Authentication of Olive and Other Vegetables oils. New Analytical Methods. Doctoral thesis. Berlin, Germany : Springer Heidelberg, p. 217. ISBN 978-3-642-31417-9

Guntarti, A., Martono, S., Yuswanto, A., and Rohman, A. 2015. FTIR Spectroscopy in Combination with Chemometrics for Analysis of Wild boar Meat in Meatball Formulation. Asian Journal of Biocemisty, vol. 10, no. 4, p. 165-172. DOI:

Guntarti, A., Martono, S., Yuswanto, A., Rohman, A. 2017. Analysis of Beef Meatball Adulteration with Wild Boar Meat using Real-Time Polymerase Chain Reaction. International Food Research Journal, vol. 24, no. 6, p. 2451-5245.

Guntarti, A., Ahda, M., Sunengsih, N. 2019. Identification of Lard on Grilled Beef Sausage Product and Steamed Beef Sausage Product using Fourier Transform Infrared (FTIR) Spectroscopy with Chemometric Combination. Potravinarstvo Slovak journal of Food Science, vol. 13, no. 1, p. 767-772. DOI:

Jaswir, I., Saeed, M. E., Torla, H. Zaki, M. 2003, Determination of lard in mixture of body fats of mutton and cow by Fourier Transform Infrared Spectroscopy. Journal of Oleo Science. vol. 52, no. 12, p. 633-638. DOI:

Lumakso, F. A., Riyanto, S., Ahmad, S. A. S., Rosman, A. S., Yusoff, F. M., Rohman, A. 2015. Application of Chemometrics in Combination with Fourier Transform Mid Infrared Spectroscopy for Authentication of Avocado Oil. Journal of Food Pharmaceutical Science., vol. 3, no. 1, p. 12-17.

Miller, J. N., Miller, J. C. 2010. Statistic and Chemometrics for Analytical Chemistry, 6th edition. Prentice Hall: England.

Naes, T., Isaksson, T., Fearn, T. and Davies, T. 2004. A User Friendly Guide to Multivariate Calibration and Classification. NIR Publication, Chichester: UK. p. 254, ISBN:9780952866626

Nunes, C. A. 2014. Vibrational spectroscopy and chemometrics to assess authenticity, adulteration and intrinsic quality parameters of edible oils and fats. Food Research International, vol. 60, p. 255-261. DOI:

Nurrulhidayah, A. F., Che Man, Y. B., Rohman, A., Amin, I., Shuhaimi, M., Khatib, A. 2013. Authentication Analysis of Butter from Beef Fat using Fourier Transform Infrared (FTIR) Spectroscopy Coupled with Chemometrics. International Food Research Journal, vol. 20, no. 3, p. 1383-1388.

Rahayu, W. S., Martono, S., Sudjadi, Rohman, A. 2018. The potential use of infrared spectroscopy and multivariate analysis for differentiation of beef meatball from dog meat for Halal authentication analysis. Journal of Advanced Veterinary and Animal Research, vol. 5, p. 307-314. DOI:

Rahmania, H., S., Rohman, A. 2015. The employment of FTIR spectroscopy in combination with chemometrics for analysis of rat meat in meatball formulation. Meat Science, vol. 100, p. 301-305. DOI:

Rahmawati, S. R., Sudjadi T. R., Rohman, A. 2016. Analysis of Pork Contamination in Abon using Mitochondrial DLoop22 Primers using Real Time Polymerase Chain Reaction Method. International Food Research Journal, vol. 23, no. 1, p. 370-374.

Regenstein, J. M., Chaudry, M. M., Regenstein, C. E. 2003. The Kosher and Halal Food Laws, Comprehensive Reveiws in Food Science and Food Safety. vol. 2, no. 3, p. 111-127. DOI:

Rohman, A., Che Man, Y. B. 2010. FTIR Spectroscopy Combined with Chemometrics for Analysis of Lard in the Mixtures with Body Fats of Lamb, Cow, and Chicken. International Food Research Journal, vol. 17, p. 519-526.

Rohman, A., Triyana, K., Sismidari, Erwanto, Y. 2012a. Differentiation of Lard and Other Animal Fats Based on Triacylglycerols Composition and Principal Component Analysis. International Food Research Journal, vol. 19, no. 2, p. 475-479.

Rohman, A., Triyana, K., Retno, S., Sismindari, Yuny, E., Tridjoko, W. 2012b. Fourier Transform Infrared Spectroscopy Applied for Rapid Analysis of Lard in Palm Oil. International Food Research Journal, vol. 19, no. 3, p. 1161-1165.

Rohman, A., Setyaningrum, D. L., Riyanto, S. 2014. FTIR Spectroscopy Combined with Partial Least Square for Analysis of Red Fruit Oil in Ternary Mixture System. International Journal of Spectroscopy, 2014, p. 1-5. DOI:

Sim, S. F., Chai, M. X. L., Kimura, A. L. J. 2018. Prediction of Lard in Palm Olein Oil Using Simple Linear Regression (SLR), Multiple Linear Regression (MLR), and Partial Least Squares Regression (PLSR) Based on Fourier-Transform Infrared (FTIR). Journal of Chemistry, 2018, p. 1-8. DOI:

Syahariza, Z., Che Man, Y. B., Selamat, J., Bakar, J. 2005. Detection of lard adulteration in cake formulation by Fourier transform infrared (FTIR) spectroscopy. Food Chemistry. vol. 92, no. 2, p. 365-371. DOI:

Van der Spiegel, M., van der Fels-Klerx, H. J., Sterrenburg, P., van Ruth, S. M., Scholtens-To-ma, I. M. J., Kok, E. J. 2012. Halal assurance in food supply chains: Verification of halal certifi-cates using audits and laboratory analysis. Trends in Food Science & Technology, vol. 27, p. 109-119. DOI:

Yang, L., Wu, T., Liu, Y., Zou, J., Huang, Y., Babu V., S., Lin, L. 2018. Rapid Identification of Pork Adulterated in the Beef and Mutton by Infrared Spectroscopy. Journal of Spectroscopy, 2018, p. 1-10. DOI:

Zhao, Q., Yang, K., Li, W., Xing, B. 2014. Concentration-Dependent Polyparameter Linear Free Energy Relationships to Predict Organic Compound Sorption on Carbon Nanotubes. Scientific Reports, vol. 4, no. 1, p. 1-7. DOI:



How to Cite

Guntarti, A., Ahda, M., Kusbandari, A., & Natalie, F. . (2020). Analysis of pork adulteration in the corned products using FTIR associated with chemometrics analysis. Potravinarstvo Slovak Journal of Food Sciences, 14, 1042–1046.