Eva Green Real-time PCR used to detect celery as an allergen in food

Ondrej  Škultéty; Andrea  Jurčáková

doi:10.5219/138

Authors

Ondrej Škultéty Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia
Andrea Jurčáková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia

DOI:

https://doi.org/10.5219/138

Keywords:

apium graveolens, food analysis, detection limit, polymerase chain reaction

Abstract

EvaGreen® Real-Time PCR method has been used for celery(Apium graveolens) allergen detection. A primer designed in mannitol dehydrogenase gene region has been used for specific celery identification in sample. The results show possibility to create calibration curve using artificially adulterated samples. The increasing variability between parallel calibration of celery samples has been observed from 0.1 % to 100%. Detection limit has been set to value 0.1% in celery representing 1000 ppm. Fluorescent signal has been presented even in samples with lower percentage addition of celery but these samples have been excluded according to unspecific

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References

BAJZÍK, P., GOLIAN, J., ŽIDEK, R., ČAPLA, J., BELEJ, Ľ., ONDREJKA, M., MRÁZOVÁ, Ľ., MARŠÁLKOVÁ, L. 2010. Methods for fish species identification in food products, In Potravinarstvo, vol. 4, 2010, no. 2, p. 1-5.

https://doi.org/10.5219/25 DOI: https://doi.org/10.5219/25

BOŠIAK, M., ŽIDEK, R., GOLIAN, J. 2009. Soy quantification on food products by real-time polymerase chain reaction. In Potravinarstvo, vol. 3, 2009, no. 1, p. 3-5.

DOVIČOVIČOVÁ, Ľ., OLEXOVÁ, L., PANGALLO, D., SIEKEL, P., KUCHTA, T. 2004. Polymerase chain reaction (PCR) for detection of celery (Apium graveolens) in food. In Eur. Food. Res. Technol., vol. 218, 2004, p. 493-495.

https://doi.org/10.1007/s00217-004-0899-0 DOI: https://doi.org/10.1007/s00217-004-0899-0

HIRD, H., LLOYD, J., GOODIER, R., BROWN, J., REECE, P.2003. Detection of peanut using real-time polymerase chain reaction. In Eur. Food. Res. Technol., vol. 217, 2003, p. 265-268.

https://doi.org/10.1007/s00217-003-0726-z DOI: https://doi.org/10.1007/s00217-003-0726-z

HUPFER, CH., WAIBLINGER, H. U., BUSCH, U. 2007. Development and validation of a real-time PCR detection method for celery in food. In Eur. Food Res. Technol., vol. 225, 2007, p. 329-335.

https://doi.org/10.1007/s00217-006-0418-6 DOI: https://doi.org/10.1007/s00217-006-0418-6

ISACSSON, J., CAO H., OHLSSON, L., NORDGREN, S., SVANVIK, N., WESTMAN, G., KUBISTA, M., SJOBACK, R., SEHLSTEDT, U. 2000. Rapid and specific detection of PCR products using light-up probes. In Molecular and Cellular Probes, vol. 14, 2000, p. 321-328.

https://doi.org/10.1006/mcpr.2000.0321 DOI: https://doi.org/10.1006/mcpr.2000.0321

PMid:11040096

JOHANSSON, S., HOURIHANE, J., BOUSQUET, J., BRUIJNZEEL-KOOMEN, C., DREBORG, S., HAAHTELA, T., KOWALSKI, M., MYGIND, N., RING, J., VAN CAUWENBERGE, P., VAN HAGE-HAMSTEN, M., WÜTHRICH, B. 2001. A revised nomenclature for allergy. In Allergy, vol. 56, 2001, p. 813-824.

https://doi.org/10.1034/j.1398-9995.2001.t01-1-00001.x DOI: https://doi.org/10.1034/j.1398-9995.2001.t01-1-00001.x

PMid:11551246

JURČÁKOVÁ, A., REVÁK, O., ŠKULTÉTY, O. 2011. Molecular – genetics methods for the determination of celery (Apium Graveolens) as an allergen in food. In Potravinarstvo, vol. 5, 2011, Special issue, p. 134-136.

PAFUNDO, S., GULLI, M., NELSON, M. 2009. SYBR®GreenER™ Real-Time PCR to detect almond in traces in processed food. In Food Chemistry, vol. 116, 2009, p. 811-815.

https://doi.org/10.1016/j.foodchem.2009.03.040 DOI: https://doi.org/10.1016/j.foodchem.2009.03.040

REVÁK, O., ŽIDEK, R., GOLIAN, J. 2010. Lupina biela ako nová hrozba pre alergikov a jej stanovanie pomocou optimalizovanej PCR reakcie. In Food Hygiene and Technology 40th Lenfeld s and Hokls Days, Brno, p. 155-158 ISBN 978-80-7305-121-1.

SAMPSON, H. A. 2003. Anaphylaxis and emergency treatment. In Pediatrics, vol. 11, 2003, p.1601-1608. DOI: https://doi.org/10.1542/peds.111.S3.1601

SAMPSON, H. A. 2004. Update on food allergy. In J. Allergy Clin. Immunol., vol. 113, 2004, p.805-819.

https://doi.org/10.1016/j.jaci.2004.03.014 DOI: https://doi.org/10.1016/j.jaci.2004.03.014

PMid:15131561

STEPHAN, O., Vieths, S. 2004. Development of a real-time PCR and a sandwich ELISA for detection of potentially allergenic trace amounts of peanut (Arachis hypogaea) in processed foods. In J. Agric. Food Chem., vol. 52, 2004, p. 3754-3760.

https://doi.org/10.1021/jf035178u DOI: https://doi.org/10.1021/jf035178u

PMid:15186093

VAN HENGEL, A. J. 2007. Food allergen detection methods and the challenge to protect food-allergic consumers. In Anal Bioanal. Chem., vol. 389, 2007, p.111-118.

https://doi.org/10.1007/s00216-007-1353-5 DOI: https://doi.org/10.1007/s00216-007-1353-5

PMid:17530230

WU, Y., CHEN, Y., WANG, B., GAO, Y., BAI, L., WANG, H.2010. SYBR Green Real-Time PCR used to detect celery in food. In Journal of AOAC international, vol. 93, 2010, no. 5, p. 1530-1536. DOI: https://doi.org/10.1093/jaoac/93.5.1530

PMid:21140666

ZELEŇÁKOVÁ, L., ŽIDEK, R., ČANIGOVÁ, M., PAULOV, J., GALLISOVÁ, T. 2010. Evaluation of Elisa method to detection of cow-lactoglobulin in sheep milk and sheep milk products. In Potravinarstvo, vol. 4, 2010, no. 4, p. 80-84. https://doi.org/10.5219/78 DOI: https://doi.org/10.5219/78