Evaluation of genetic diversity of edible honeysuckle monitored by RAPD in relation to bioactive substances

Authors

  • Marcela Cehula Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Botany and Genetics, Nábrežie mládeže 91, 949 74 Nitra, Slovak Republic, Tel.: +421376408581 https://orcid.org/0000-0002-8048-1144
  • Tünde Jurí­ková Constantine the Philosopher University, Faculty of Central European Studies, Institute for Teacher Training, Dražovská 4, 949 74 Nitra, Slovakia, Tel.: +421376408855 https://orcid.org/0000-0002-8286-8262
  • Jana Žiarovská Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Plant Breeding, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414244 https://orcid.org/0000-0002-0005-9729
  • Jiří­ Mlček Tomas Bata University in Zlí­n, Faculty of Technology, Department of Food Analysis and Chemistry, nám. T.G. Masaryka 5555, 760 01 Zlí­n, Czech Republic, Tel.: +420576033030 https://orcid.org/0000-0002-5753-8560
  • Matúš Kyseľ Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Genetics and Plant Breeding, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421907045799 https://orcid.org/0000-0002-1679-391X

DOI:

https://doi.org/10.5219/1139

Keywords:

honeysuckle, RAPD, DPPH, Folin-Ciocalteu, total polyphenols content

Abstract

The aim of this study was clarifying the relation between genetic diversity of edible honeysuckle (Lonicera kamtschatica) and the major group of biologically active substances as total polyphenols content (TPC) including antioxidant activity (AO). Fruits of edible honeysuckle becomes more and more popular, especially in Europe. The current status of research on polyphenolic compounds in the berries of edible honeysuckle and their biological effects, including recommended utilization, are reviewed.The biological material including 14 cultivars of the edible honeysuckle (´Zoluška´, ´Amfora´, ´Pruhonický 44´, ´Vasilijevsky´, ´Moskovskaja´, ´Vojtek´, ´Sinoglaska´, ´Altaj´, ´Lipnická´, ´Kamčadalka´, ´Sinaja Ptica´, ´Fialka´, ´Modrý Triumf´, and ´Leningradský velikán´) originated from Czech republic (Žabcice near Brno). The content of TPC and AO were determined by location and its soil-climatic conditions and these environmental circumstances determines the RAPD profiles of analysed honeysuckle acessions, too. DPPH method was used to analyze AO and Folin-Ciocalteu method was used to determine TPC. The results of experiment showed that the highest value of AO was determined at the cultivars ´Zoluška´ (81.04 mg.L-1) and the lowest was measured in ´Kamčadalka´ (54.122 mg.L-1).  On the contrary, the highest content of TPC was determined at the cultivar ´Kamčadalka´ (51.09 mg.L-1) and the lowest value was measured at the cultivar ´Pruhonický 44´ (21.65 mg.L-1). Phylogenetic trees were similar in genetic distance. The  content of TPC and AO were  not statistically significant in relation to cultivar. The analyzed cultivars of the edible honeysuckle were separated in 4 clusters according to used primers. In both  gel images, the amplicon size ranged from 100 to 1,500 bp. We found that genetic diversity was partially related to content of total polyphenolic substances and antioxidant activity. Based on phylogenetic trees we have stated that variety ´Lipnická´, ´Sinoglaska´, ´Altaj´, ´Leningradský velikán´, ´Modrý Triumf´, ´Sinaja Ptica´ and ´Kamčadalka´ were grouped in the similar cluster. The highest genetic distance was determined at the variety ´Vasilijevskaja´ and ´Amfora´. In the same way, there were variety ´Vojtek´, ´Fialka´ and ´Zoluška´.

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Published

2019-06-28

How to Cite

Cehula, M., Jurí­ková, T., Žiarovská, J., Mlček, J., & Kyseľ, M. (2019). Evaluation of genetic diversity of edible honeysuckle monitored by RAPD in relation to bioactive substances. Potravinarstvo Slovak Journal of Food Sciences, 13(1), 490–496. https://doi.org/10.5219/1139

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Vol. 13 No. 1 (2019): Potravinarstvo Slovak Journal of Food Sciences

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Articles

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This license permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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