Evaluation of storage methods of beef by microbiological and chemical indicators
DOI:
https://doi.org/10.5219/1381Keywords:
psychrotrophic microflora, microbiological criteria, beef chilled, beef frozen, volatile fatty acids of meatAbstract
Meat and meat products are a major part of a person's ration. However, due to their high nutritional value, they are a favorable environment for the development of microorganisms and require refrigerated storage. The purpose of this work was to evaluate the storage methods for refrigerated and frozen beef by microbiological and chemical parameters and to suggest criteria for evaluating beef by the content of psychrotrophic microorganisms. It was found out that the storage of beef meat with an initial mesophilic bacterial content of about 4.88 log CFU.cm-2 of surface and psychrotrophic bacteria 3.79 log CFU.cm-2 at temperature 0 °C is only possible for 8 days, further, the microbiological indices exceed the acceptable standards. Investigation of the dynamics of microflora reproduction during the storage of beef in the frozen state at temperature -2 to -3 °C for 20 days established a decrease in 1.3 times the number of mesophilic bacteria in 10 days of storage. At the same time, the number of psychrotrophic microorganisms during this storage time was increased in 4.5 times, and 20 days in 7.9 times and amounted to 5.3 log CFU.cm-2 of surface area. This indicates that the storage of meat in the frozen state inhibits or completely stops the development of mesophilic microorganisms for 20 days. It was found out that storing of beef in the cooled state at a temperature of 0 ±0.5 °C for more than eight days is impractical, as its biochemical indices are worsening and signs of spoilage are appearing. At the same time, storing of beef in the frozen state at a temperature of -2 to -3 °C for 20 days does not cause such significant biochemical changes as in beef stored in the cooled state at a temperature of 0 ±0.5 °C for 16 days. Therefore, we have experimentally substantiated the quantitative indicators of the content of psychrotrophic microorganisms on the surface of beef intended for storage in a cooled or frozen state. The proposed microbiological criteria will improve the safety of beef.
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Adam, K. H., Flint, S. H., Brightwell, G. 2010. Psychrophilic and psychrotrophic clostridia: sporulation and germination processes and their role in the spoilage of chilled, vacuum-packaged beef, lamb and venison. International Journal of Food Science and Technology, vol. 45, no. 8, p. 1539-1544. https://doi.org/10.1111/j.1365-2621.2010.02320.x DOI: https://doi.org/10.1111/j.1365-2621.2010.02320.x
Alonso-Hernando, A., Alonso-Calleja, C., Capita, R. 2013. Growth kinetic parameters of gram-positive and gram-negative bacteria on poultry treated with various chemical decontaminants. Food Control, vol. 33, no. 2, p. 429-432. https://doi.org/10.1016/j.foodcont.2013.03.009 DOI: https://doi.org/10.1016/j.foodcont.2013.03.009
Alonso-Hernando, A., Capita, R., Alonso-Calleja, С. 2013. Decontamination Treatments for Psychrotrophic Microorganisms on Chicken Meat during Storage at Different Temperatures. Journal of Food Protection, vol. 76, no. 11, p. 1977-1980. https://doi.org/10.4315/0362-028X.JFP-13-175 DOI: https://doi.org/10.4315/0362-028X.JFP-13-175
Bruckner, S., Albrecht, A., Petersen, B., Kreyenschmidt, J. 2012. Characterization and comparison of spoilage processes in fresh pork and poultry. Journal of Food Quality, vol. 35, no. 5, p. 372-382. https://doi.org/10.1111/j.1745-4557.2012.00456.x DOI: https://doi.org/10.1111/j.1745-4557.2012.00456.x
Cantalejo, M. J., Zouaghi, F., Pérez-Arnedo, I. 2016. Combined effects of ozone and freeze-drying on the shelf-life of Broiler chicken meat. LWT-Food Science and Technology, vol. 68, p. 400-407. https://doi.org/10.1016/j.lwt.2015.12.058 DOI: https://doi.org/10.1016/j.lwt.2015.12.058
Casaburi, A., De Filippis, F., Villani, F., Ercolini, D. 2014. Activities of strains of Brochothrix thermosphacta in vitro and in meat. Food Research International, vol. 62, p. 366-374. https://doi.org/10.1016/j.foodres.2014.03.019 DOI: https://doi.org/10.1016/j.foodres.2014.03.019
Cerveny, J., Meyer, J. D., Hall, P. A. 2009. Microbiological spoilage of meat and poultry products. In Sperber, W., Doyle, M. Compendium of the microbiological spoilage of foods and beverages. Food Microbiology and Food Safety. NEW YORK, USA : Springer, p. 69-86. ISBN 978-1-4419-0826-1. https://doi.org/10.1007/978-1-4419-0826-1_3 DOI: https://doi.org/10.1007/978-1-4419-0826-1_3
Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs (Text with EEA relevance) OJ L 338, 22.12.2005, p. 1-26.
Dave, D., Ghaly, A. E. 2011. Meat Spoilage Mechanisms and Preservation Techniques: A Critical Review. American Journal of Agricultural and Biological Sciences, vol. 6, no. 4, p. 486-510. https://doi.org/10.3844/ajabssp.2011.486.510 DOI: https://doi.org/10.3844/ajabssp.2011.486.510
Doulgeraki, A. I., Ercolini, D., Villani, F., Nychas, G. J. E. 2012. Spoilage microbiota associated to the storage of raw meat in different conditions. International Journal of Food Microbiology, vol. 157, no. 2, p. 130-141. https://doi.org/10.1016/j.ijfoodmicro.2012.05.020 DOI: https://doi.org/10.1016/j.ijfoodmicro.2012.05.020
DSTU. 2009. DSTU 6030:2008, Meat beef and veal in carcasses, semicarcasses and qurters. Specification. National Standard of Ukraine.
Ercolini, D., Russo, F., Nasi, A., Ferranti, P., Villani, F. 2009. Mesophilic and Psychrotrophic Bacteria from Meat and Their Spoilage Potential In Vitro and in Beef. Applied and Environmental Microbiology, vol. 75, no 7, p. 1990-2001. https://doi:10.1128/AEM.02762-08 DOI: https://doi.org/10.1128/AEM.02762-08
Gunvig, A., Hansen, F., Borggaard, C. 2013. A mathematical model for predicting growth/no-growth of psychrotrophic C. botulinum in meat products with five variables. Food Control, vol. 29, no. 2, p. 309-317. https://doi.org/10.1016/j.foodcont.2012.06.046 DOI: https://doi.org/10.1016/j.foodcont.2012.06.046
Hassan, M. A., Shaltout, F. A., Maarouf, A. A., El-Shafey, W. S. 2015. Psychrotrophic bacteria in frozen fish with special reference to pseudomonas species. Benha Veterinary Medical Journal, vol. 27, no. 1, p. 78-83.
Hilgarth, M., Behr, J., Vogel, R. F. 2018. Monitoring of spoilage-associated microbiota on modified atmosphere packaged beef and differentiation of psychrophilic and psychrotrophic strains. Journal of Applied Microbiology, vol. 124, no. 3, p. 740-753. https://doi.org/10.1111/jam.13669 DOI: https://doi.org/10.1111/jam.13669
ISO. 2017a. ISO 6887-1:2017, Microbiology of the food chain – Preparation of test samples, initial suspension and decimal dilutions for microbiological examination – Part 1: General rules for the preparation of the initial suspension and decimal dilutions. International Standard Organisation.
ISO. 2017b. ISO 6887-2:2017, Microbiology of the food chain – Preparation of test samples, initial suspension and decimal dilutions for microbiological examination – Part 2: Specific rules for the preparation of meat and meat products. International Standard Organisation.
Jay, J. M., Loessner, M. J., Golden, D. A. 2005. Modern Food Microbiology, 7th ed. NEW YORK, USA : Springer Science and Business Media, p. 63-101. ISBN 978-0-387-23413-7.
Jeremiah, L. E. 1997. Extension of chilled pork storage life. Natoinal Pork Producers Coucill, no. 4, p. 1-8.
Kameník, J. 2013. The microbiology of meat spoilage: a review. Maso International Journal of Food Science and Technology, vol. 1, p. 003-010.
Kukhtyn, M., Kravcheniuk, K., Beyko, L., Horiuk, Y., Skliar, O., Kernychnyi, S. 2019. Modeling the process of microbial biofilm formation on stainless steel with a different surface roughness. Eastern-European Journal of Enterprise Technologies, vol. 2, no. 11, p. 14-21. https://doi.org/10.15587/1729-4061.2019.160142 DOI: https://doi.org/10.15587/1729-4061.2019.160142
Kukhtyn, M., Salata, V., Pelenyo, R., Selskyi, V., Horiuk, Y., Boltyk, N., Ulko, L., Dobrovolsky, V. 2020. Investigation of zeranol in beef of Ukrainian production and its reduction with various technological processing. Potravinarstvo Slovak Journal of Food Sciences, vol. 14, no. 1, p. 95-100. https://doi.org/10.5219/1224 DOI: https://doi.org/10.5219/1224
Lerma, L. L., Benomar, N., del Carmen Casado Muñoz, M., Gálvez, A., Abriouel, H. 2015. Correlation between antibiotic and biocide resistance in mesophilic and psychrotrophic Pseudomonas spp. isolated from slaughterhouse surfaces throughout meat chain production. Food Microbiology, vol. 51, p. 33-44. https://doi.org/10.1016/j.fm.2015.04.010 DOI: https://doi.org/10.1016/j.fm.2015.04.010
Leroy, F., Vasilopoulos, C., Van Hemelryck, S., Falony, G., G., De Vuyst, L. 2009. Volatile analysis of spoiled, artisan-type, modified-atmosphere-packaged cooked ham stored under different temperatures. Food Microbiology, vol. 26, no 1, p. 94-102. https://doi:10.1016/j.fm.2008.08.005 DOI: https://doi.org/10.1016/j.fm.2008.08.005
Maas-van Berkel, B., van den Boogaard, B., Heijnen, C. 2004. Preservation of fish and meat. WAGENINGEN, The Netherlands : Agromisa Foundation, 86 p. ISBN: 90-72746-01-9.
Mayr, D., Margesin, R., Klingsbichel, E., Hartungen, E., Jenewein, D., Schinner, F., Märk, T. D. 2003. Rapid detection of meat spoilage by measuring volatile organic compounds by using proton transfer reaction mass spectrometry. Applied and Environmental Microbiology, vol. 69, no. 8, p. 4697-4705. https://doi.org/10.1128/AEM.69.8.4697-4705.2003 DOI: https://doi.org/10.1128/AEM.69.8.4697-4705.2003
Morales, P., Fernández-García, E., Nuñez, M. 2005. Production of volatile compounds in cheese by P. fragi strains of Dairy origin. Journal of Food Protection, vol. 68, no. 7, p. 1399-1407. https://doi.org/10.4315/0362-028x-68.7.1399 DOI: https://doi.org/10.4315/0362-028X-68.7.1399
Moschonas, G., Bolton, D. J., McDowell, D. A., Sheridan, J. J. 2011. Diversity of Culturable Psychrophilic and Psychrotrophic Anaerobic Bacteria Isolated from Beef Abattoirs and Their Environments. Applied and Environmental Microbiology, vol. 77, no. 13, p. 4280-4284. https://doi.org/10.1128/AEM.01778-10 DOI: https://doi.org/10.1128/AEM.01778-10
Nieminen, T. T., Vihavainen, E., Paloranta, A., Lehto, J., Paulin, L., Auvinen, P., Solismaa, M., Björkroth, K. J. 2011. Characterization of psychrotrophic bacterial communities in modified atmosphere-packed meat with terminal restriction fragment length polymorphism. International Journal of Food Microbiology, vol. 144, no. 3, p. 360-366. https://doi.org/10.1016/j.ijfoodmicro.2010.10.018 DOI: https://doi.org/10.1016/j.ijfoodmicro.2010.10.018
Nyamakwere, F., Muchenje, V., Mushonga, B., Makepe, M., Mutero, G. 2016. Assessment of Salmonella, Escherichia Coli, Enterobacteriaceae and Aerobic Colony Counts Contamination Levels During the Beef Slaughter Process. Journal of Food Safety, vol. 36, no. 4, p. 548-556. https://doi.org/10.1111/jfs.12275 DOI: https://doi.org/10.1111/jfs.12275
Padda, R. S., Pandey, K. K., Kaul, S., Nair, V. D., Jain, R. K., Basu, S. K., Chakrabarti, T. 2001. A novel gene encoding a 54 kDa polypeptide is essential for butane utilization by the GenBank accession number for the orf54 sequence is L81125. Pseudomonas sp. IMT37. Microbiology, vol. 147, no. 9, p. 2479-2491. https://doi.org/10.1099/00221287-147-9-2479 DOI: https://doi.org/10.1099/00221287-147-9-2479
Pennacchia, C., Ercolini, D., Villani, F. 2011. Spoilage-related microbiota associated with chilled beef stored in air or vakuum pack. Food Microbiology, vol. 28, no. 1, p. 84-93. https://doi.org/10.1016/j.fm.2010.08.010 DOI: https://doi.org/10.1016/j.fm.2010.08.010
Popelka, P., Jevinová, P., Marcinčák, S. 2016. Microbiological and chemical quality of fresh and frozen whole trout and trout fillets. Potravinarstvo, vol. 10, no. 1, p. 431-436. https://doi.org/10.5219/599 DOI: https://doi.org/10.5219/599
Pothakos, V., Samapundo, S., Devlieghere, F. 2012. Total mesophilic counts underestimate in many cases the contamination levels of psychrotrophic lactic acid bacteria (LAB) in chilled-stored food products at the end of their shelf-life. Food Microbiology, vol. 32, no. 2, p. 437-443. https://doi.org/10.1016/j.fm.2012.07.011 DOI: https://doi.org/10.1016/j.fm.2012.07.011
Pothakos, V., Taminiau, В., Huys, G., Nezer, С., Daube, G., Devlieghere, F. 2014. Psychrotrophic lactic acid bacteria associated with production batch recalls and sporadic cases of early spoilage in Belgium between 2010 and 2014. International Journal of Food Microbiology, vol. 191, p. 157-163. https://doi.org/10.1016/j.ijfoodmicro.2014.09.013 DOI: https://doi.org/10.1016/j.ijfoodmicro.2014.09.013
Robertson, M., Hapca, S. M., Moshynets, O., Spiers, A. J. 2013. Air-liquid interface biofilm formation by psychrotrophic pseudomonads recovered from spoilt meat. Antonie van Leeuwenhoek, vol. 103, p. 251-259. https://doi.org/10.1007/s10482-012-9796-x DOI: https://doi.org/10.1007/s10482-012-9796-x
Salata, V., Kuhtyn, M., Semanjuk, V., Perkij, Y. 2017. Dynamics of microflora of chilled and frosted beef during storage. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, vol. 19, no. 73, p. 178-182. https://doi.org/10.15421/nvlvet7337 DOI: https://doi.org/10.15421/nvlvet7337
Serraino, A., Bardasi, L., Riu, R., Pizzamiglio, V., Liuzzo, G., Galletti, G., Giacometti, F., Merialdi, G. 2012. Visual evaluation of cattle cleanliness and correlation to carcass microbial contamination during slaughtering. Meat Science, vol. 90, no. 2, p. 502-506. https://doi.org/10.1016/j.meatsci.2011.08.001 DOI: https://doi.org/10.1016/j.meatsci.2011.08.001
Stellato, G., Utter, D. R., Voorhis, A., De Angelis, M., Eren, A. M., Ercolini, D. 2017. A Few Pseudomonas Oligotypes Dominate in the Meat and Dairy Processing Environment. Frontiers in Microbiology, vol. 8, 9 p. https://doi.org/10.3389/fmicb.2017.00264 DOI: https://doi.org/10.3389/fmicb.2017.00264
Vega, V. A., Anzulovich, A. C., Varas, S. M., Bonomi, M. R., Giménez, M. S., Oliveros, L. B. 2009. Effect of nutrinional vitamin A deficiency on lipid metabolism in the rat heart: Its relation to PPAR gene expression. Nutrition, vol. 25. no. 7-8, p. 828-838. https://doi.org/ 10.1016/j.nut.2009.01.008 DOI: https://doi.org/10.1016/j.nut.2009.01.008
Vos, P., Garrity, G., Jones, D., Krieg, N. R., Ludwig, W., Rainey, F. A., Whitman, W. B. 2011. Bergey's manual of systematic bacteriology: Volume 3: The Firmicutes (Vol. 3). NEW YORK, USA : Springer Science & Business Media. ISBN-978-0-387-95041-9.
Wei, Q., Wang, X., Sun, D. W., Pu, H. 2019. Rapid detection and control of psychrotrophic microorganisms in cold storage foods: A review. Trends in Food Science & Technology, vol. 86, p. 453-464. https://doi.org/10.1016/j.tifs.2019.02.009 DOI: https://doi.org/10.1016/j.tifs.2019.02.009
Zhang, Y., Wei, J., Yuan, Y., Yue, T. 2019. Diversity and characterization of spoilage-associated psychrotrophs in food in cold chain. International Journal of Food Microbiology, vol. 290, p. 86-95. https://doi.org/10.1016/j.ijfoodmicro.2018.09.026 DOI: https://doi.org/10.1016/j.ijfoodmicro.2018.09.026
Zhou, G. H., Xu, X. L., Liu, Y. 2010. Preservation technologies for fresh meat-A review. Meat Science, vol. 86, no. 1, p. 119-128. https://doi.org/10.1016/j.meatsci.2010.04.033 DOI: https://doi.org/10.1016/j.meatsci.2010.04.033
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