Microscopic fungi isolated from different Slovak grape varieties

Authors

  • Miroslava Kačániová
  • Attila Kántor Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Plant products storage and processing, Tr. A. Hlinku 2, 949 76 Nitra
  • Margarita Terentjeva Latvia University of Agriculture, Faculty of Veterinary Medicine Institute of Food and Environmental Hygiene, K. Helmaņa iela 8, LV-3004, Jelgava
  • Soňa Felsöciová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. A. Hlinku 2, 949 76 Nitra
  • Eva Ivanišová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Plant products storage and processing, Tr. A. Hlinku 2, 949 76 Nitra
  • Maciej Kluz Faculty of biology and agriculture, University of Rzeszow, Department of Bioenergy Technology and Food Analysis, Zelwerowicza St. 4, 35-601 Rzeszow
  • Pawel Hanus Faculty of biology and agriculture, University of Rzeszow, Department of Bioenergy Technology and Food Analysis, Zelwerowicza St. 4, 35-601 Rzeszow
  • Czeslaw Puchalski Faculty of biology and agriculture, University of Rzeszow, Department of Bioenergy Technology and Food Analysis, Zelwerowicza St. 4, 35-601 Rzeszow
  • Miriam Kádasi Horáková Slovak Academy of Sciences, Institute of Forest Ecology, Ľ. Štúra 2, 960 53 Zvolen
  • Miroslava Kačániová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Microbiology, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/893

Keywords:

grape, microscopic fungi, Alternaria, Penicillium, the Lesser Carpathian region

Abstract

The aim of this study was to isolate and identify microscopic fungi in different grape samples. We collected 13 grapes varienties samples (9 white and 4 red) from local Slovak winemakers in the end of the September 2017. Used 13 grape samples in this study: Alibernet, Irsai Oliver, Dornfelder, Blue Frankish, Feteasca regala, Green Veltliner, Pálava, Mūller Thurgau, Rhinriesling, Cabernet Savignon, Pinot Blanc, Savignon Blanc and Welschriesling. Microscopic fungi in grape samples were detected on Malt extract agar by spread plate method. The number of microscopic fungi ranged from 2.85 log cfu.g-1 in Cabernet Savignon to 4.83 log cfu.g-1 in Feteasca regala. A total of 627 isolates of microscopic fungi were obtained in this study. The most abundant fungi belonged to genera Alternaria and Penicillium (100% frequency). The high frequency was also detected for Aspergillus (76.92%) and Cladosporium (76.92%) but with lesser relative density. Alternaria sp., Aspergillus niger, Aspergillus sp., Botrytis cinerea, Cladosporium sp., Penicillium expansum, Phoma sp., Rhizopus sp. and Trichoderma sp. species were isolated from grape berries.

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References

Abe, L. T., Da Mota, R. V., Lajolo, F. M., Genovese, M. I., 2007. Compostos fenólicos ecapacidade antioxidante de cultivares de uvas Vitis labrusca L. e Vitis vinifera L. Ciência e Tecnologia de Alimentos, vol. 27, no. 2, p. 394-400. https://doi.org/10.1590/s0101-20612007000200032 DOI: https://doi.org/10.1590/S0101-20612007000200032

Abrunhosa L., Paterson R. R., Kozakiewicz Z., Lima N., Venancio A. 2001. Mycotoxin production from fungi isolated from grapes. Letters of Applied Microbiology, vol. 32, no. 4, p. 240-242. https://doi.org/10.1046/j.1472-5x.2001.00897.x DOI: https://doi.org/10.1046/j.1472-765X.2001.00897.x

Bau, M., Bragulat, M. R., Abarca, M. L., Minguez, S., Cabañes, F. J., 2005. Ochratoxigenic species from Spanish wine grapes. International Journal of Food Microbiology, vol. 98, no. 2, p. 125-130. https://doi.org/10.1016/j.ijfoodmicro.2004.05.015 DOI: https://doi.org/10.1016/j.ijfoodmicro.2004.05.015

Felšöciová, S,. Tančinová, D., Rybárik, Ľ., Mašková, Z. Mycobiota of Slovak wine grapes with emphasis on aspergillus and penicillium species in the south slovak wine region. Potravinarstvo Slovak Journal of Food Sciences, vol. 11, no. 1, p. 496-502. https://dx.doi.org/10.5219/789 DOI: https://doi.org/10.5219/789

Freire, L., Passamani, F. R. F., Thomas, A. B., Nassur, R. S. M. R., Silva, L. M., Paschoal, F. N., Pereira, G. E., Prado, G., Batista, L. R. Influence of physical and chemical characteristics of wine grapes on the incidence of Penicillium and Aspergillus fungi in grapes and ochratoxin A in wines. International Journal of Food Microbiology, vol. 241, p. 181-190. https://doi.org/10.1016/j.ijfoodmicro.2016.10.027 DOI: https://doi.org/10.1016/j.ijfoodmicro.2016.10.027

Gautam, A., Sharma, S., Bhadauria, R. 2009. Detection of toxigenic fungi and mycotoxins in medicinally important powdered herbal drugs. The Internet Journal of Microbiology, vol. 7, no. 2, p. 1-8. https://doi.org/10.5580/104b DOI: https://doi.org/10.5580/104b

González, H. H. L., Martinez, E. J., Pacin, A., Resnik, S. L. 1999. Relationship between Fusarium graminearum and Alternaria alternata contamination and deoxinivalenol occurrence on Argentinian durum wheat. Mycopathologia, vol. 144, no. 2, p. 97-102. https://doi.org/10.1023/A:1007020822134 DOI: https://doi.org/10.1023/A:1007020822134

La Guerche, S., Chamont, S., Blancard, D., Dubourdieu, D., Darriet, P. 2005. Origin of (-)-geosmin on grapes: on the complementary action of two fungi, botrytis cinerea and penicillium expansum. Antonie Van Leeuwenhoek, vol. 88, no. 2, p. 131-139. https://doi.org/10.1007/s10482-005-3872-4 DOI: https://doi.org/10.1007/s10482-005-3872-4

Lorenzini, M., Zapparoli, G., 2014a. Characterization and pathogenicity of Alternaria spp. strains associated with grape bunch rot during post-harvest withering. International Journal of Food Microbiology, vol. 186, p. 1-5. https://doi.org/10.1016/j.ijfoodmicro.2014.06.008 DOI: https://doi.org/10.1016/j.ijfoodmicro.2014.06.008

Lorenzini, M., Zapparoli, G., 2014b. An isolate morphologically and phylogenetically distinct from Botrytis cinerea obtained from withered grapes possibly represents a new species of Botrytis. Plant Pathology, vol. 63, no. 6, p. 1326-1335. https://doi.org/10.1111/ppa.12216 DOI: https://doi.org/10.1111/ppa.12216

Lorenzini, M., Zapparoli, G., 2015. Occurrence and infection of Cladosporium, Fusarium, Epicoccum and Aureobasidium in withered rotten grapes during post-harvest dehydration. Antonie Van Leeuwenhoek, vol. 108, no. 5, p. 1171-1180. https://doi.org/10.1007/s10482-015-0570-8 DOI: https://doi.org/10.1007/s10482-015-0570-8

Lorenzini, M., Cappello, M. S., Zapparoli, G., 2015. Isolation of Neofusicoccum parvum from withered grapes: strain characterization, pathogenicity and its detrimental effects on passito wine aroma. Journal of Applied Microbiology, vol. 119, no. 5, p. 1335-1344. https://doi.org/10.1111/jam.12931 DOI: https://doi.org/10.1111/jam.12931

Lorenzini, M., Zapparoli, G., 2016. Description of taxonomically unde fined Sclerotiniaceae strain from withered rotten-grapes. Antonie Van Leeuwenhoek, vol. 109, no. 2, p. 197-205. https://doi.org/10.1007/s10482-015-0621-1 DOI: https://doi.org/10.1007/s10482-015-0621-1

Lorenzini, M., Cappello, M. S., Logrieco, A., Zapparoli, G. 2016. Polymorphism and phylogenetic species delimitation in filamentous fungi from predominant mycobiota in withered grapes. International Journal of Food Microbiology, vol. 238, p. 56-62. https://doi.org/10.1016/j.ijfoodmicro.2016.08.039 PMid:27591387 DOI: https://doi.org/10.1016/j.ijfoodmicro.2016.08.039

Mencarelli, F., Tonutti, P. 2013. Sweet, Reinforced and Fortified Wines. Chichester, UK : John Wiley & Sons, Ltd. https://doi.org/10.1002/9781118569184 DOI: https://doi.org/10.1002/9781118569184

Medina, A., Mateo, R., López-Ocaña, L., Valle-Algarra, F. M., Jiménez, M., 2005. Study of spanish grape mycobiota and ochratoxin A production by isolates of Aspergillus tubingensis and other members of Aspergillus section Nigri. Applied and Environmental Microbiology, vol. 71, no. 8, p. 4696-4702. https://doi.org/10.1128/aem.71.8.4696-4702.2005 DOI: https://doi.org/10.1128/AEM.71.8.4696-4702.2005

Narayanasam, P., 2006. Postharvest Pathogens and Disease Management. New York, NY : John Wiley & Sons, Inc., 578 p. ISBN: 9780471751984. DOI: https://doi.org/10.1002/0471751987

Pitt, J. I., Hocking, A. D. 2009. Fungi and Food Spoilage. 3rd ed. London, UK : Springer. 519 p. ISBN 978-0-387-92206-5. https://doi.org/10.1007/978-0-387-92207-2 DOI: https://doi.org/10.1007/978-0-387-92207-2

Ribereau-Gayon, P., Glories, Y., Maujean, A., Dubourdieu, D. 1998. Handbook of Enology, New York, NY : John Wiley & Sons, Ltd., p. 265-321.

Romero, S. M., Patriarca, A., Fernández Pinto, V., Vaamonde, G., 2007. Effect of water activity and temperature on growth of ochratoxigenic strains of Aspergillus carbonarius isolated from Argentinean dried vine fruits. International Journal of Food Microbiology, vol. 115, no. 2, p. 140-143. https://doi.org/10.1016/j.ijfoodmicro.2006.10.014 PMid:17161486 DOI: https://doi.org/10.1016/j.ijfoodmicro.2006.10.014

Rousseaux, S., Diguta, C. F., Radoï-Matei, F., Alexandre, H., Guilloux-Bénatier, M., 2014. Non-Botrytis grape-rotting fungi responsible for earthy and moldy off-fl avors and mycotoxins. Food Microbiology, vol. 38, p. 104-121. https://doi.org/10.1016/j.fm.2013.08.013 DOI: https://doi.org/10.1016/j.fm.2013.08.013

Samson, R. A., Frisvad, J. C. 2004. Polyphasic taxonomy of Penicillium subgenus Penicillium: new taxonomic schemes and mycotoxins and other extrolites. Utrecht: Centraalbureau voor Schimmelcultures. 260 p. ISBN 90-70351-53-6.

Samson, R. A., Hoekstra, E. S., Frisvad, J. C., Filtenborg, O. 2002a. Introduction to food- and airborne fungi. 6th revised ed. (with some corrections). Utrecht, Netherland : Centraalbureau voor Schimmelcultures. 389 p. ISBN 90-70351-42-0.

Sage, L., Garon, D., Seigle-Murandi, F., 2004. Fungal microflora and ochratoxin a risk in French vineyards. Journal of Agricultural and Food Chemistry, vol. 52, no. 18, p. 5764-5768. https://doi.org/10.1021/jf049497c DOI: https://doi.org/10.1021/jf049497c

Serra, R., Lourenço, A., Alipio, P., Venâncio, A. 2006. Influence of the region of origin on the mycobiota of grapes with emphasis on Aspergillus and Penicillium species. Mycological Research, vol. 110, no. 8, p. 971-978. https://doi.org/10.1016/j.mycres.2006.05.010 DOI: https://doi.org/10.1016/j.mycres.2006.05.010

Serra, R., Braga, A., Venâncio, A., 2005. Mycotoxin-producing and other fungi isolated from grapes for wine production, with particular emphasis on ochratoxin A. Research in Microbiology, vol. 156, no. 4, p. 515-521. https://doi.org/10.1016/j.resmic.2004.12.005 DOI: https://doi.org/10.1016/j.resmic.2004.12.005

Valero, A., Marìn, S., Ramos, A. J., Sanchis, V., 2005. Ochratoxin A-producing species in grapes and sun-dried grapes and their relation to ecophysiological factors. Letters in Applied Microbiology, vol. 41, no. 2, p. 196-201. https://doi.org/10.1111/j.1472-765x.2005.01705.x DOI: https://doi.org/10.1111/j.1472-765X.2005.01705.x

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Published

2018-04-17

How to Cite

Kačániová, M. ., Kántor, A. ., Terentjeva, M. ., Felsöciová, S. ., Ivanišová, E. ., Kluz, M. ., Hanus, P. ., Puchalski, C. ., Kádasi Horáková, M. ., & Kačániová, M. . (2018). Microscopic fungi isolated from different Slovak grape varieties. Potravinarstvo Slovak Journal of Food Sciences, 12(1), 438–443. https://doi.org/10.5219/893

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