Microbial biofilms produced by pseudomonas fluorescens on solid surfaces

Jozef  Čapla; Peter  Zajác; Jozef  Golian; Pavol  Bajzí­k; Lucia  Zeleňáková; Vladimí­r  Vietoris; Dagmar  Kozelová

doi:10.5219/18

Authors

Jozef Čapla Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra
Peter Zajác Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra
Jozef Golian Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra
Pavol Bajzík Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra
Lucia Zeleňáková Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra
Vladimír Vietoris Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Storing and Processing Plant Products, Tr. A. Hlinku 2, 949 76 Nitra
Dagmar Kozelová Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/18

Keywords:

biofilm, microbial attachment, Pseudomonas fluorescens

Abstract

A biofilm is a complex aggregation of microorganisms growing on a solid substrate. Biofilms are characterized by structural heterogeneity, genetic diversity, complex community interactions, and an extracellular matrix of polymeric substances. The experimental part was focused on the adhesion of bacterial cells under static conditions and testing the effectiveness of disinfectants on created biofilm. In laboratory conditions we prepared and formed the bacterial biofilms Pseudomonas fluorescens in the four test surfaces of stainless steel, glass and plastic materials - PE (polyethylene) and EPDM (ethylene propylene diene monomer). Over the next 72 hours and 72 hours were observed numbers of adhesion bacterial cells of P. fluorescens on solid surfaces of tested materials. The highest values adhesion cells reached P. fluorescens cells after 72 hours of cultivation on plastic surfaces, where was increased in adhesion bacterial cells for EPDM in the values of 10⁵ CFU/cm² and for PE up to 10⁶ CFU/cm². The subsequent repeated 72-hour cultivation P. fluorescens was an increase (growth) in the number of adhesion bacterial cells to all tested surfaces.

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