Optimalisation of species identification of common carp (Cyprinus carpio) using SYBR® green I real-time PCRmethod

Authors

  • Pavol Bajzí­k Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Radoslav Židek Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Golian Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Ľubomí­r Belej Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Jozef Čapla Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Lenka Maršálková Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra
  • Ondrej Revák Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra

DOI:

https://doi.org/10.5219/154

Keywords:

species identification, SYBR® Green I. Real-Time PCR, Cyprinus carpio

Abstract

European Union Member States, together with a number of countries around the world, places great emphasis on ensuring the protection of consumers as a potential food allergic from food allergies. to inform consumers that their product may contain any of the risk of allergenic ingredients. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR® Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (Cyprinus carpio), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. Results of using the optimized SYBR ® Green I Real-Time PCR method for species identification common carp (Cyprinus carpio) indicate to its suitability.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

ASENSIO, L., MONTERO, A., 2008. Analysis of fresh fish labeling in Spanish fish retail shops. In Food Control, vol. 19, 2008, p. 795-799. https://doi.org/10.1016/j.foodcont.2007.08.005 DOI: https://doi.org/10.1016/j.foodcont.2007.08.005

ATAMAN, C., CELIK U., REHBEIN H., 2006. Identification of some Aegean fish species by native isoelectric focusing. In European Food Research and Technology, vol. 222, 2006, p. 99-104. https://doi.org/10.1007/s00217-005-0149-0 DOI: https://doi.org/10.1007/s00217-005-0149-0

BOYE, J. I., 2010. Allergen management in the food industry. New Jersey : Wiley, 2010. 624 p. ISBN 978-0-470-22735-0. https://doi.org/10.1002/9780470644584 DOI: https://doi.org/10.1002/9780470644584

CIVERA, T., 2003. Species identification and safety of fish products. In Veterinary Research Communications, vol. 27, 2003, p. 481-489. https://doi.org/10.1023/B:VERC.0000014205.87859.ab PMid:14535455 DOI: https://doi.org/10.1023/B:VERC.0000014205.87859.ab

DIETRICH, M. A., NYNCA, J,. BILIŃSKA, B., KUBA, J., KOTULA-BALAK, M., KAROL, M., CIERESZKO, A., 2010. Identification of parvalbumin-like protein as a major protein of common carp (Cyprinus carpio L) spermatozoa which appears during final stage of spermatogenesis. In Comparative Biochemistry and Physiology, Part B, vol. 157. 2010, p. 220-227. https://doi.org/10.1016/j.cbpb.2010.06.007 PMid:20601062 DOI: https://doi.org/10.1016/j.cbpb.2010.06.007

GIL, L. A., 2007. PCR-based methods for fish and fishery products authentication. In Food Science & Technology, vol. 18, 2007, p. 558-566. https://doi.org/10.1016/j.tifs.2007.04.016 DOI: https://doi.org/10.1016/j.tifs.2007.04.016

HIRD, H. J., HOLD, G. L., CHRISHOLM, J., REECE, P., RUSSELL, V. J., BROWN, J., 2005. Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR. In European Food Research and Technology, vol. 220, 2005, p. 633-637. https://doi.org/10.1007/s00217-004-1050-y DOI: https://doi.org/10.1007/s00217-004-1050-y

HORSTKOTTE, B., REHBEIN, H., 2003. Fish species identification by means of restriction fragment length polymorphism and high performance liquid chromatography. In Journal of Food Science, vol. 68, 2003, p. 2658-2666. https://doi.org/10.1111/j.1365-2621.2003.tb05785.x DOI: https://doi.org/10.1111/j.1365-2621.2003.tb05785.x

HUBALKOVA, Z., KRALIK, P., TREMLOVA, B., 2007. Methods of gadoid fish species identification in food and their economic impact in the Czech Republic: DOI: https://doi.org/10.17221/2044-VETMED

a review. In Veterinary Medicine, vol. 52, 2007, p. 273-292.

KVASNIČKA, F., 2005. Capillary electrophoresis in food authenticity. In Journal of Separation Science. vol. 28, 2005, p. 813-825. https://doi.org/10.1002/jssc.200500054 PMid:16013809 DOI: https://doi.org/10.1002/jssc.200500054

LOCKLEY, A. K., BARDSLEY, R. G., 2000. DNA-based methods for food authentication. In Trends in Food Science and Technology, vol.11, 2000, p. 67-77. https://doi.org/10.1016/S0924-2244(00)00049-2 DOI: https://doi.org/10.1016/S0924-2244(00)00049-2

MACKIE, I. M., 1996. Authenticity of fish. P. R. Ashurt, M. J. Dennis (Eds.), In Food authentication, London: Blackie Academic and Professional, 1996, p.140-170. DOI: https://doi.org/10.1007/978-1-4613-1119-5_5

MORETTI, V. M., TURCHINI, G. M., BELLAGAMBA, F., 2003. Traceability issues in fishery and aquaculture products. In Veterinary Research Communications, vol. 27, 2003, p. 497-505. https://doi.org/10.1023/B:VERC.0000014207.01900.5c PMid:14535457 DOI: https://doi.org/10.1023/B:VERC.0000014207.01900.5c

PASCUAL, C. Y., RECHE, M., FIANDOR, A., VALBUENA, T., CUEVAS, T., MARTIN-ESTEBAN, M. M., 2008. Fish allergy in childhood. In Pediatric Allergy and Immunology, vol. 19, 2008, p. 573-579. https://doi.org/10.1111/j.1399-3038.2008.00822.x PMid:18950323 DOI: https://doi.org/10.1111/j.1399-3038.2008.00822.x

POMS, R. E., KLEIN, C. L., ANKLAM, E., 2004. Methods for allergen analysis in food: a review. In Food Additives & Contaminants, vol. 21, 2004, p. 1-31. https://doi.org/10.1080/02652030310001620423 PMid:14744677 DOI: https://doi.org/10.1080/02652030310001620423

RIMÁROVÁ, K., LOVAYOVÁ, V., 2007. Alergény a lepok v potravinách, prístupy k ich detekcii a označovaniu v EÚ. In: HYGIENA ALIMENTORUM XXVIII „Bezpečnosť a kvalita mlieka a mliečnych výrobkov". Slovakia : Štrbské Pleso - Vysoké Tatry, 2007, p. 165-169. ISBN 978-80-8077-055-6.

SICHERER, S. H., SAMPSON, H. A., 2010. Food allergy. In Journal of Allergy and Clinical Immunology, vol. 125, 2010, p. S116-S126. https://doi.org/10.1016/j.jaci.2009.08.028 PMid:20042231 DOI: https://doi.org/10.1016/j.jaci.2009.08.028

TELETCHEA, T., MAUDET, C., HÄNNI, C. 2005. Food and forensic molecular identification: update and challenges. In Trends in Biotechnology, vol. 23, 2005, p. 359-366. https://doi.org/10.1016/j.tibtech.2005.05.006 PMid:15927295 DOI: https://doi.org/10.1016/j.tibtech.2005.05.006

TROTTA, M., SCHÖNHUTH, S., PEPE, T., CORTESI, M. L., PUYET, A., BAUTISTA, J. M., 2005. A multiplex - PCR Metod for use in real - time PCR for identification of fish fillets from grouper (Epinephelus spp. and Mycteroperca spp.) and common substitute species. In Journal of Agricultural and Food Chemistry, vol. 53, 2005, p. 2039 - 2045. https://doi.org/10.1021/jf048542d PMid:15769133 DOI: https://doi.org/10.1021/jf048542d

VAN HENGEL, A. J., 2007. Food allergen detection methods and the challenge to protect food-allergic consumers. In Analytical and Bioanalytical Chemistry, vol. 389, 2007, p. 111-118. https://doi.org/10.1007/s00216-007-1353-5 PMid:17530230 DOI: https://doi.org/10.1007/s00216-007-1353-5

ŽIDEK, R., GOLIAN, J., 2008. Detection of carp (Cyprinius carpio) proteins using genetic markers. In Proteiny : Sborník příspěvků V. ročníku medzinárodní konference. Zlín : Univerzita Tomáše Bati ve Zlíne, 2008, p.235-237.

Downloads

Published

2011-07-03

How to Cite

Bajzí­k, P. ., Židek, R. ., Golian, J. ., Belej, Ľubomí­r ., Čapla, J. ., Maršálková, L. ., & Revák, O. . (2011). Optimalisation of species identification of common carp (Cyprinus carpio) using SYBR® green I real-time PCRmethod. Potravinarstvo Slovak Journal of Food Sciences, 5(3), 1–5. https://doi.org/10.5219/154

Issue

Vol. 5 No. 3 (2011): Potravinarstvo

Section

Articles

License

This license permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

Crossref
Scopus
Google Scholar
Europe PMC

Most read articles by the same author(s)

<< < 1 2 3 4 5 6 7 8 > >>