Persistence of L. monocytogenes versus adherence on solid surface

Authors

  • Janka Koreňová Food research Institute in Bratislava, Department of Microbiology, Molecular Biology and Biotechnology, Priemyselná 4, P.O.Box 25, 824 75 Bratislava 26
  • Katarí­na Oravcová Food research Institute in Bratislava, Department of Microbiology, Molecular Biology and Biotechnology, Priemyselná 4, P.O.Box 25, 824 75 Bratislava 26

DOI:

https://doi.org/10.5219/135

Keywords:

biofilm, Listeria monocytogenes, persistence

Abstract

Listeria monocytogenes is an important food-borne pathogen that frequently persists in food-processing environments despite rigorous sanitation procedures. Specific phenotypes that have been linked to persistence have been previously investigated but no clear association has been demonstrated. In this study we characterised four persistent L. monocytogenes strains isolated from food factories in Austria, Czech Republic, Denmark and Ireland in comparison with four non-persistent counterparts. The L. monocytogenes isolates were analysed for the ability to form biofilm during exponential/stationary growth phase at different temperature at low nutrition stress and adhesion to polystyrene at high-salinity conditions. Persistent and non-persistent strains did not significantly differ in the ability to form biofilm or to adhere to polystyrene microtitre plate.

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References

BORUCKI, M. K., PEPPIN, J. D., WHITE, D., LOGE, F., AND CALL, D. R. 2003. Variation in biofilm formation among strains of Listeria monocytogenes. In Appl. Environ. Microbiol., vol. 69, 2003, p. 7336-7342.

https://doi.org/10.1128/AEM.69.12.7336-7342.2003 DOI: https://doi.org/10.1128/AEM.69.12.7336-7342.2003

PMid:14660383

CALY, D., TAKILT, D., LEBRET, V. AND TRESSE, O. 2009. Sodium chloride affects Listeria monocytogenes adhesion to polystyrene and stainless steel by regulating flagella expression. In Lett. Appl. Microbiol., vol. 49, 2009, p. 751-756.

https://doi.org/10.1111/j.1472-765X.2009.02735.x DOI: https://doi.org/10.1111/j.1472-765X.2009.02735.x

PMid:19793195

CHAVANT, P., MARTINIE, B., MEYLHEUC, T., BELLON-FONTAINE, M. N., HEBRAUD, M. 2002. Listeria monocytogenes LO28: Surface physicochemical properties and ability to form biofilms at different teperatures and growth phases. In Appl. Environ. Microbiol., vol. 68, 2002, p. 728-737.

https://doi.org/10.1128/AEM.68.2.728-737.2002 DOI: https://doi.org/10.1128/AEM.68.2.728-737.2002

PMid:11823213

DEAN, R. B., DIXON, W. J. 1951. Simplified statistics for small numbers of observations. In Anal. Chem., vol. 23, 1951, p. 636-638.

https://doi.org/10.1021/ac60052a025 DOI: https://doi.org/10.1021/ac60052a025

DJORDJEVIC, D., WIEDMANN, M., MCLANDSBOROUGH, L. A. 2002. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. In Appl. Environ. Microbiol., vol. 68, 2002, p. 2950-2958.

https://doi.org/10.1128/AEM.68.6.2950-2958.2002 DOI: https://doi.org/10.1128/AEM.68.6.2950-2958.2002

PMid:12039754

FARBER, J. M., PETERKIN, P. I. 1991. Listeria monocytogenes, a food-borne pathogen. In Microbiol. Rev., vol. 55, 1991, p. 476‑511.) DOI: https://doi.org/10.1128/mr.55.3.476-511.1991

PMid:1943998

GRINSTEAD, D. 2009. Cleaning and sanitation in food processing environments for the prevention of biofilm formation, and biofilm removal. In Biofilms in the Food and Beverage Industries ed. Fratamico, P. M., Annous B. A. and Gunther IV, N. W. pp. 331-358. Cambridge: Woodhead Publishing.

https://doi.org/10.1533/9781845697167.3.331 DOI: https://doi.org/10.1533/9781845697167.3.331

KALMOKOFF, M. L., AUSTIN, J. W., WAN, X. D., SANDERS, G., BANERJEE, S., FARBER, J. M. 2001. Adsorption, attachment and biofilm formation among isolates of Listeria monocytogenes using model conditions. In J. Appl. Microbiol., vol. 91, 2001, p. 725-734.

https://doi.org/10.1046/j.1365-2672.2001.01419.x DOI: https://doi.org/10.1046/j.1365-2672.2001.01419.x

PMid:11576310

KOREŇOVÁ, J., LOPAŠOVSKÁ, J., KUCHTA, T. 2009. Biofilm forming bacterial contaminants in small and medium-sized ewes' milk and meat processing enterprises in Slovakia. In J. Food Nutr. Res., vol. 48, 2009, p. 115-120.

LELIEVELD, H. L. M. 2006. Sources of contamination. In Hygiene in Food Processing ed. Lelieveld, H. L. M, Mostert, M.A. and White, B, pp. 61-75. Cambridge: Woodhead Publishing. DOI: https://doi.org/10.1016/9781855737051.2.61

LIANOU, A., SOFOS, J. N. 2007. A review of the incidence and transmission of Listeria monocytogenes in ready-to-eat products in retail and food service environments. In J. Food Prot., vol. 70, 2007, p. 2172-2198. DOI: https://doi.org/10.4315/0362-028X-70.9.2172

https://doi.org/10.1016/j.fm.2006.11.002 DOI: https://doi.org/10.1016/j.fm.2006.11.002

PMid:17900099

LUNDÉN, J., AUTIO, T., KORKEALA, H. 2003. Persistent and nonpersistent Listeria monocytogenes contamination in meat and poultry processing plants. In J. Food Prot., vol. 66, 2003, p. 2062-2069. DOI: https://doi.org/10.4315/0362-028X-66.11.2062

PMid:14627284

MORETRO, T., LANGSRUD, S. 2004. Listeria monocytogenes: biofilm formation and persistence in food-processing environments. In Biofilms, vol. 1, 2004, p. 107-121.

https://doi.org/10.1017/S1479050504001322 DOI: https://doi.org/10.1017/S1479050504001322

RODRIGUES, D. A., ALMEIDA, M. A., TEIXEIRA, P. A., OLIVEIRA, R. T. AND AYEREDO, J. C. 2009. Effect of batch and fed-batch growth modes on biofilm formation by Listeria monocytogenes at different temperatures. In Curr. Microbiol., vol. 59, 2009, p. 457-462.

https://doi.org/10.1007/s00284-009-9460-5 DOI: https://doi.org/10.1007/s00284-009-9460-5

PMid:19653035

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Published

2011-03-06

How to Cite

Koreňová, J. ., & Oravcová, K. . (2011). Persistence of L. monocytogenes versus adherence on solid surface. Potravinarstvo Slovak Journal of Food Sciences, 5(2), 41–44. https://doi.org/10.5219/135