Chemical composition of chicken meat after application of humic acid and probiotic Lactobacillus fermentum
DOI:
https://doi.org/10.5219/943Keywords:
chemical composition, chicken meat, humic acids, dietery supplemationAbstract
The aim of the present study was analysed and evaluated chemical parameters of chicken breast and thigh muscles after addition of humic acids and probiotic into diet for broiler chicken. A total of 200 pcs Ross 308 broiler chickens were divided into 4 groups (n=50). The control group of chickens was fed with complete feed mixtures without any additives. Chickens in experiment groups were fed a diet containing: P1 (1% of humic acid), P2 (1% of humic acid and probiotic supplement Lactobacillus fermentum) and P3 were fed with complete feed mixture containing combination of starter feed mixture (1. - 21. day) with coccidiostaticum Diclazuril and growth feed mixture (21. - 35. day) containing Salinomycinum sodium. Besides, the groups were kept under the same conditions. Fattening period lasted for 42 days. Chicken meat was analyzed for content of water, crude protein, fat and cholesterol. Based on the results, we can state that the application of humic acids or the combination of Humac Natur with probiotic did not affect the chemical composition of the breast muscle. In the breast muscle, the protein content in the experimental group P3 with the coccidiostat (22.98 g.100 g-1) was reduced (p ≤0.05) compared to control group (23.42 g.100 g-1). In the case of thigh muscle was significantly higher content of fat and cholesterol (p ≤0.05) in chickens feeding with addition of Humac Natur (fat - 9.08 g.100g-1; cholesterol - 0.86 mg.100g-1) and similar results were recorded in experimental group with combination of Humac Natur and probiotic (fat - 9.15 g.100g-1; cholesterol - 0.86 mg.100g-1) compared to control group (fat - 7.15 g.100g-1; cholesterol - 0.70 mg.100g-1). From a general point of view, we can recommend the application of Humac Natur, respectively combination Humac Natur with probiotics in feeding of broiler chickens Ross 308.
Downloads
Metrics
References
Abdel-Latif, K. O., El-Husseiny, O. M., Abdallah, A. G. 2008. The Influence of Biological Feed Additives on Broiler Performance. International Journal of Poultry Science, vol. 7, no. 9, p. 862-871. https://doi.org/10.3923/ijps.2008.862.871 DOI: https://doi.org/10.3923/ijps.2008.862.871
Ahmed, S. T., Islam, M. M., Bostami, A. B. M. R., Mun, H., Kim, Y., Yang. C. 2015. Meat composition, fatty acid profile and oxidative stability of meat from broilers supplemented with pomegranate (Punica granatum L.) by-products. Food Chemistry, vol. 188, p. 481-488. https://doi.org/10.1016/j.foodchem.2015.04.140 DOI: https://doi.org/10.1016/j.foodchem.2015.04.140
Alkhalf, A., Alhaj, M., Al-Homidan, I. 2010. Influence of probiotic supplementation on blood parameters and growth performance in broiler chickens. Saudi Journal of Biological Sciences, vol. 17, no. 3, p. 219-225. https://doi.org/10.1016/j.sjbs.2010.04.005 DOI: https://doi.org/10.1016/j.sjbs.2010.04.005
Angelovičová, M., Alfaig, E., Král, M., Tkáčová, J. 2013. The effect of the probiotics Bacillus Subtilis (PB6) on the selected indicators of the table eggs quality, fat and cholesterol. Potravinarstvo, vol. 7, no. 1, p. 80-84. https://doi.org/10.5219/271 DOI: https://doi.org/10.5219/271
Bai, S. P., Wu, A. M., Sing, X. M., Lei, Y., Bai, J., Zhang, K. Y., Chio, J. S. 2013. Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens. Poultry Science, vol. 92, no. 3, p. 663-670. https://doi.org/10.3382/ps.2012-02813 DOI: https://doi.org/10.3382/ps.2012-02813
Bajaj, B., Claes, I., Lebeer, S. 2015. Functional mechanisms of probiotics. Journal Of Microbiology, Biotechnology and Food Sciences, vol. 4, no. 4, p. 321-327. https://doi.org/10.15414/jmbfs.2015.4.4.321-327 DOI: https://doi.org/10.15414/jmbfs.2015.4.4.321-327
Bernardeau. M., Vernoux, J. 2013. Overview of differences between microbial feed additives and probiotics for food regarding regulation, growth promotion effects and health properties and consequences for extrapolation of farm animal results to humans. Clinical Microbiology and Infection, vol. 19, no. 4, p. 321-330. https://doi.org/10.1111/1469-0691.12130 DOI: https://doi.org/10.1111/1469-0691.12130
Blajman, J., Gaziano, C., Zbrun, M. V., Soto, L., Astesana, D., Berisvil, A., Scharpen, Signorini, M., Frizzo, L. 2015. In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens. Research in Veterinary Science, vol. 101, p. 50-56. https://doi.org/10.1016/j.rvsc.2015.05.017 DOI: https://doi.org/10.1016/j.rvsc.2015.05.017
Bobko, M., Haščík, P., Bobková, A., Pavelková, A., Tkáčová, J., Trembecká, L. 2015. Lipid oxidation in chicken meat after application of bee pollen extract, propolis extract and probiotic in their diets. Potravinarstvo, vol. 9, no. 1, p. 342-346. https://doi.org/10.5219/495 DOI: https://doi.org/10.5219/495
Brenes E., Roura A. 2010. Essential oils in poultry nutrition: Main effects and modes of action. Animal Feed Science and Technology. 2010. vol. 158, no. 1-2, p. 1-14. https://doi.org/10.1016/j.anifeedsci.2010.03.007 DOI: https://doi.org/10.1016/j.anifeedsci.2010.03.007
Ceylan, N., Ciftci, I., Ilhan, Z. 2003. Effects of Some Alternative Feed Additives for Antibiotic Growth Promoters on Performance and Gut Microflora of Broilers. Turkish Journal of Veterinary and Animal Sciences, vol. 27, no. 3, p. 727-733.
Corzo, A., Fritts, C. A., Kidd, M. T., Kerr, B. J. 2005. Response of broiler chicks to essential and nonessential amino acid supplementation of low crude protein diets. Animal Feed Science and Technology, vol. 118, no. 3-4, p. 319-327. https://doi.org/10.1016/j.anifeedsci.2004.11.007 DOI: https://doi.org/10.1016/j.anifeedsci.2004.11.007
Daneshmand, A., Sadeghi, G. H., Karimi, A., Vaziry, A., Ibrahim, S. A. 2015. Evaluating complementary effects of ethanol extract of propolis with the probiotic on growth performance, immune response and serum metabolites in male broiler chickens. Livestock Science, vol. 178, p. 195-201. https://doi.org/10.1016/j.livsci.2015.04.012 DOI: https://doi.org/10.1016/j.livsci.2015.04.012
El-Husseiny, O. M., Abdallah, A. G., Abdel-Latif, K. O. 2008. The influence of biological feed additives on broiler performance. International Journal of Poultry Science, vol. 7, no. 9, p. 862-871. https://doi.org/10.3923/ijps.2008.862.871 DOI: https://doi.org/10.3923/ijps.2008.862.871
Enberg, R. M., Hedemann. M. S., Leser, T. D., Jensen, B. B. 2000. Effect of Zinc Bacitracin and Salinomycin on Intestinal Microflora and Performance of Broilers. Poultry Science, vol. 79, no. 9, p. 1311-1319. https://doi.org/10.1093/ps/79.9.1311 DOI: https://doi.org/10.1093/ps/79.9.1311
Eren, M., Deniz, G., Gezen, S. S., Türkmen, I. I. 2000. Broyler yemlerine kat lan humatlar n besiperformans, serum mineral konsantrasyonu ve kemik külü üzerine etkileri. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 47, no. 3, p. 255-263. https://doi.org/10.1501/Vetfak_0000000463 DOI: https://doi.org/10.1501/Vetfak_0000000463
Giannenas, I., Papadopoulos, E., Tsalie, E., Triantafillou, E., Henikl, S., Teichmann, K., Tontis, D. 2012. Assessment of dietary supplementation with probiotics on performance, intestinal morphology and microflora of chickens infected with Eimeria tenella. Veterinary Parasitology, vol. 188, no. 1-2, p. 31-40. https://doi.org/10.1016/j.vetpar.2012.02.017 DOI: https://doi.org/10.1016/j.vetpar.2012.02.017
Gomez, S., Angeles, M. L., Mojica, M. C., Jalukar, S. 2012. Combination of an enzymatically hydrolyzed yeast and yeast culture with a direct-fed microbial in the feeds of broiler chickens. Asian-Australasian Journal of Animal Sciences, vol. 25, no. 5, p. 665-673. https://doi.org/10.5713/ajas.2011.11316 DOI: https://doi.org/10.5713/ajas.2011.11316
Gous, R. M. 2010. Nutritional limitations on growth and development in poultry. Livestock Science, vol. 130, no. 1-3, p. 25-32. https://doi.org/10.1016/j.livsci.2010.02.007 DOI: https://doi.org/10.1016/j.livsci.2010.02.007
Haščík, P., Trembecká, L., Bobko, M., Kačániová, M., Čuboň, J., Kunová, S., Bučko, O. 2016. Effect of diet supplemented with propolis extract and probiotic additives on performance, carcass characteristics and meat composition of broiler chickens. Potravinarstvo, vol. 10, 2016, no. 1, p. 223-231. https://doi.org/10.5219/581 DOI: https://doi.org/10.5219/581
Haščík, P., Trembecká, L., Bobko, M., Čuboň, J., Bučko, O., Tkáčová, J. 2015. Evaluation of Meat Quality after Application of Different Feed Additives in Diet of Broiler Chickens. Potravinarstvo, vol. 9, no. 1, p. 174-182. https://doi.org/10.5219/429 DOI: https://doi.org/10.5219/429
Islam, K. M. S., Schuhmacher, A., Gropp, J. M. 2005. Humic acid substances in animal agriculture. Pakistan Journal of Nutrition, vol. 4, no. 3, p. 126-134. https://doi.org/10.3923/pjn.2005.126.134 DOI: https://doi.org/10.3923/pjn.2005.126.134
Joo, S. T., Kim, G. D, Hwang, Y. H., Ryu, Y. C. 2013. Control of fresh meat quality through manipulation of muscle fiber characteristics. Meat Science, vol. 95, no. 4, p. 828-836. https://doi.org/10.1016/j.meatsci.2013.04.044 DOI: https://doi.org/10.1016/j.meatsci.2013.04.044
Kabir, S. M. L., Rahman, M. M., Rahman, M. B., Rahman, M. M., Ahmed, S. U. 2004. The Dynamics of Probiotics on Growth Performance and Immune Response in Broilers. International Journal of Poultry Science, vol. 3, no. 5, p. 361-364. https://doi.org/10.3923/ijps.2004.361.364 DOI: https://doi.org/10.3923/ijps.2004.361.364
Karaoglu, M., Aksu, M. I., Esenbuga, N., Kaya, M., Macit, M., Durdag, H. 2004. Effect of dietary probiotic on the pH and colour characteristics of carcasses, breast fillets and drumsticks of broilers. Animal Science, vol. 78, no. 2, p. 253-259. https://doi.org/10.1080/10942910500267364 DOI: https://doi.org/10.1017/S1357729800054047
Khaksefidi, A., Rahimi, S. H. 2005. Effect of Probiotic Inclusion in the Diet of Broiler Chickens on Performance, Feed Efficiency and Carcass Quality. Asian-Australasian Journal of Animal Sciences, vol. 18, no. 8, p. 1153-1156. https://doi.org/10.5713/ajas.2005.1153 DOI: https://doi.org/10.5713/ajas.2005.1153
Kluth H., Rodehutscord M. 2006. The role of methodical aspects in the study of aminoacid subacute digestibility with poultry (Metodinių aspektų reikšmė amino rūgščių precekalinio virškinamumo tyrimuose su naminiais paukščiais). Veterinarija ir zootechnika, vol. 35, no. 57, p. 36-42.
Kocabağli, N., Alp, M., Acar, N, Kahraman, R. 2002. The effects of dietary humate supplemantation on broiler growth and carcass. Poultry Science, vol. 81, no. 2, p. 227-230. https://doi.org/10.1093/ps/81.2.227 DOI: https://doi.org/10.1093/ps/81.2.227
Lee, K. W., Everts, H., Beynen, A. C. 2004. Essential oils in broiler nutrition. International Journal Poultry Science, vol. 3, no. 12, p. 738-752. https://doi.org/10.3923/ijps.2004.738.752 DOI: https://doi.org/10.3923/ijps.2004.738.752
Lee, K. W., Everts, H., Kappert, H. J., Frehner, M., Losa, R., Beynen, A. C. 2003. Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science, vol. 44, no. 3, p. 450-457. https://doi.org/10.1080/0007166031000085508 DOI: https://doi.org/10.1080/0007166031000085508
MacCarthy, P. 2001. The principles of humic substances. Soil Science, vol. 166, no. 11, p. 38-751. https://doi.org/10.1097/00010694-200111000-00003 DOI: https://doi.org/10.1097/00010694-200111000-00003
Miezeliene, A., Alencikiene, G., Gruzauskas, R., Barstys, T. 2011. The effect of dietary selenium supplementation on meat quality of broiler chickens. Biotechnology, Agronomy, Society and Environment, vol. 15, no. S1, p. 61-69.
Mirnawati, Y. R., Marlida, Y. 2013. Effects of humic acid addition via drinking water on the performance of broilers fed diets containing fermented and non-fermented palm kernel cake. Archiva Zootechnica, vol. 16, no. 1, p. 41-53.
Mutus, R., Kocabagli, N., Alp, M., Acar, N., Eren, M., Gezen, S. S. 2006. The effect of dietary probiotic supplementation on tibial bone characteristics and strength in broilers. Poultry Science, vol. 85, no. 9, p. 1621-1625. https://doi.org/10.1093/ps/85.9.1621 DOI: https://doi.org/10.1093/ps/85.9.1621
Nagaraju, R., Reddy, B. S. V., Gloridoss, R., Suresh, B. N., Ramesh, C. 2014. Effect of dietary supplementation of humic acids on performance of broilers. The Indian Journal of Animal Sciences, vol. 84, no. 4, p. 447-452.
Ondruška, Ľ., Chrastinová, Ľ., Rafay, J., Pospíšilová, D., Parkányi, V. 2012. Effect of humic substances and probiotics on growth performance and meat quality of rabbits. Potravinarstvo, vol. 6, no. 2, p. 39-41. https://doi.org/10.5219/192 DOI: https://doi.org/10.5219/192
O'Sullivan, M. G. 2017. A Handbook for Sensory and Consumer-Driven New Product Development. Cambridge : Woodhead Publishing. 370 p. ISBN 978-0-08-100352-7.
Ozturk, E., Ocak, N., Coskun, I., Turhan, S., Erener, G. 2010. Effects of humic substances supplementation provided through drinking water on performance, carcass traits and meat quality of broilers. Journal of Animal Physiology and Animal Nutrition, vol. 94, no. 1, p. 78-85. https://doi.org/10.1111/j.1439-0396.2008.00886.x DOI: https://doi.org/10.1111/j.1439-0396.2008.00886.x
Ozturk, E., Ocak, N., Turan, A., Erener, G., Altop, A., Cankaya, S. 2011. Performance, carcass, gastrointestinal tract and meat quality traits, and selected blood parameters of broilers fed diets supplemented with humic substances. Journal of the Science of Food and Agriculture, vol. 92, no. 1, p. 59-65. https://doi.org/10.1002/jsfa.4541 DOI: https://doi.org/10.1002/jsfa.4541
Rath, N. C., Huff, W. E., Huff, G. R. 2006. Effects of humic acid on broiler chickens. Poultry Science, 2006, vol. 85, no. 3, p. 410-414. https://doi.org/10.1093/ps/85.3.410 DOI: https://doi.org/10.1093/ps/85.3.410
Shalmany, K. S., Shivazad, M. 2006. The Effect of Diet Propolis Supplementation on Ross Broiler Chicks Performance. International Journal of Poultry Science, vol. 5, no. 1, p. 84-88. https://doi.org/10.3923/ijps.2006.84.88 DOI: https://doi.org/10.3923/ijps.2006.84.88
Reitznerová, A., Nagy, J., Marcinčák, S., Mačanga, J., Vaško, L., Vašková, J., Poláková, Z. 2016. The effect of humic acids on oxidative stability of frozen chicken. Bezpečnosť a kontrola potravín : Zborník prác z XIII. Vedeckej konferencie s medzinárodnou účasťou, Nitra : SPU, p. 182-185. ISBN 978-80-552-1481-8. (In Slovak)
Shim, Y. H., Ingale, S. L., Kim, J. S., Kim, K. H., Seo, D. K., Lee, S. C., Chae, B. J., Kwon, I. K. 2012. A multi-microbe probiotic formulation processed at low and high drying temperatures: effects on growth performance, nutrient retention and caecal microbiology of broilers. British Poultry Science, vol. 53, no. 4, p. 482-490. https://doi.org/10.1080/00071668.2012.690508 DOI: https://doi.org/10.1080/00071668.2012.690508
Taklimi, S. M. S. M., Lotfollahian, H., Shahne, A. Z., Mirzaei, F., Alinejad, A. 2012. Study on efficacy of probiotic in broiler chickens diet. Agricultural Sciences, vol. 3, no. 1, p. 5-8. https://doi.org/10.4236/as.2012.31002 DOI: https://doi.org/10.4236/as.2012.31002
Tateo, A., De Palo, P., Maggiolino, A., Centoducati, P. 2013. Post-thawing colour changes in meat of foals as affected by feeding level and post-thawing time. Archives Animal Breeding, vol. 56, no. 29, p. 293-302. https://doi.org/10.7482/0003-9438-56-029 DOI: https://doi.org/10.7482/0003-9438-56-029
Uyeno, Y., Shigemori, S., & Shimosato, T. 2015. Effect of Probiotics/Prebiotics on Cattle Health and Productivity. Microbes and Environments, vol. 30, no. 2, p. 126-132. https://doi.org/10.1264/jsme2.ME14176 DOI: https://doi.org/10.1264/jsme2.ME14176
Wang, Y., Sun, J., Zhong, H., Li, N., Xu, H., Zhu, Q., Liu, Y. 2017. Effect of probiotics on the meat flavour and gut microbiota of chicken. Scientific Reports. vol. 7, no. 1, p. 6400. https://doi.org/10.1038/s41598-017-06677-z DOI: https://doi.org/10.1038/s41598-017-06677-z
Yasar, S., Gokcimen, A., Altuntas, I., Yonden, Z., Petekkaya, E. 2002. Performance and ileal histomorphology of rats treated with humic acid preparations. Journal of Animal Physiology and Animal Nutrition, vol. 86, no. 7-8, p. 257-264. https://doi.org/10.1046/j.1439-0396.2002.00383.x DOI: https://doi.org/10.1046/j.1439-0396.2002.00383.x
Zhang, J. L., Xie, Q. M., Ji, J., Yang, W. H., Wu, Y. B., Li, C., Ma, J. Y., Bi, Y. Z. 2012. Different combinations of probiotics improve the production performance, egg quality, and immune response of layer hens. Poultry Science, vol. 91, no. 11, p. 2755-2760. https://doi.org/10.3382/ps.2012-02339 DOI: https://doi.org/10.3382/ps.2012-02339
Zralý, Z., Písaříková, B. 2010. Effect of sodium humate on the content of trace elements in organs of weaned piglets. Acta Veterinaria Brno, vol. 79, no. 1, p. 73-79. https://doi.org/10.2754/avb201079010073 DOI: https://doi.org/10.2754/avb201079010073
Downloads
Published
How to Cite
Issue
Section
License
This license permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
