Content of endogenous sulfur dioxide in wines
DOI:
https://doi.org/10.5219/854Keywords:
Endogenous sulfur dioxide, wine, reductons, iodemetric titration, spectrophotometryAbstract
Content of free and total endogenous sulfur dioxide were evaluated by classical iodometric titration in must, during winemaking processes and in bio-wine. No exogenous sulfur dioxide was added in any technological operations to simplify the evaluations. In addition, the results were corrected on the content of reductons (total content of reducing substances). The results confirmed formation of endogenous sulfur dioxide from sulfur containing substances (sulfur containing amino acids etc.) in both experiments. Microbial sulfur dioxide is preferably bound to carbonyl substances. Only minor part is present in the free (active) form of the sulfur dioxide. In addition, total content of polyphenols (TPC) and total antioxidant capacity (TAC) were determined by spectrophotometry at the same time. A procedure OIV-MA-AS323-O4B: R 2009 was used. Contents of "free" and "total" sulfur dioxide (with/without correction on contents of reductons) and total content of reductones were determined after complexing the sulfur dioxide with formaldehyde. A standard spectrophotometric method using Folin-Ciocalteu reagent was applied for determination of total content of polyphenolics (TPC) at 765 nm after 60 min incubation. The results were expressed as tannin equivalents (in mg.L-1). A standard DPPH (2,2´-difenyl-1-picrylhydrazyl dissolved in methanol) spectrophotometric method was applied for determination of total antioxidant capacity (TAC) at 515 nm. Depletion of the color intensity was measured after 60 min incubation against blank (methanol) and absorbance decrease Δ(A) = (A0 - A1)/A0 was calculated and used for construction of calibration curve. The TAC values were expressed as ascorbic acid concentrations (in mg.L-1).
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Abramovic, H., Kosmerl, T., Ulrih, N.P., Cigic B. 2015. Contribution of SO2 to antioxidant potential of white wine. Food Chemistry, vol. 174, no. 1, p. 147-153. https://doi.org/10.1016/j.foodchem.2014.11.030 DOI: https://doi.org/10.1016/j.foodchem.2014.11.030
Alexandre, H., Costello, P.J., Remize, F., Guzzo, J., Guilloux-Benatier, M. 2004. Saccharomyces cerevisiae - Oenococcus oeni interactions in wine: Current knowledge and perspectives. International Journal of Food Microbiology, vol. 93, no. 1, p. 141-154. https://doi.org/10.1016/j.ijfoodmicro.2003.10.103 DOI: https://doi.org/10.1016/j.ijfoodmicro.2003.10.013
Bajčan, D., Vollmannová, A., Šimanský, V., Bystrická, J., Trebichalský, P., Árvay, J., Czako, P. 2016. Antioxidant activity, phenolic content and colour of the Slovak cabernet sauvignon wines. Potravinarstvo, vol. 10, no. 1, p. 89-94. https://doi.org/10.5219/534 DOI: https://doi.org/10.5219/534
Carrete, R., Vidal, M. T., Bordons, A., Constanti, M. 2002. Inhibitory effect of sulfur dioxide and other stress compounds in wine on the ATPase activity of Oenococcus oeni. FEMS Microbiological Letters, vol. 211, no. 1, p. 155-159. https://doi.org/10.1111/j.1574-6968.2002.tb11218.x DOI: https://doi.org/10.1016/S0378-1097(02)00687-0
Čmelík, J., Machát, J., Niedobová, E., Otruba, V., Kanický, V. 2005, Determination of free and total sulfur dioxide in wine samples by vapour-generation inductively coupled plasma-optical-emission spectrometry. Analytical and Bioanalytical Chemistry, vol. 383, no. 3, p. 483-488. https://doi.org/10.1007/s00216-005-3382-2 DOI: https://doi.org/10.1007/s00216-005-3382-2
Eschenbruch, R. 1974. Sulfite and sulfide formation during winemaking - review. American Journal of Enology and Viticulture, vol. 25, no. 3, p. 157-161.
Dott, W., Truper, H. G. 1976. Sulfite formation by wine yeasts. 3. Properties of sulfite reductase. Archivum Microbiology, vol. 108, no. 1, p. 99-104. DOI: https://doi.org/10.1007/BF00425098
Henick-Kling, T., Park, Y. H. 1994. Considerations fort he use of yeast and bacterial starter cultures - SO2 and timing of inoculation. American Journal of Enology and Viticulture, vol. 45, no. 3, p. 464-469.
Jančářová, I., Jančář, L., Náplavová, A., Kubáň, V. 2014. A role of reductones in monitoring of the content of sulfur dioxide in wines during their maturation and storage. Czech Journal of Food Science, vol. 32, no. 3, p. 232-240. DOI: https://doi.org/10.17221/323/2013-CJFS
Kubáň, P., Jánoš, P., Kubáň, V. 1998. Gas diffusion-flow injection determination of free and total sulfur dioxide in wines by conductometry. Collection of Czech Chemical Communication, vol. 63, no. 1, p. 770-782. DOI: https://doi.org/10.1135/cccc19980770
Rankine, B. C., Pocock, K. F. 1969. Influence of yeast strain on binding of sulphur dioxides in wines and on its formation during fermentation. Journal of the Science Food Agriculture, vol. 20, no. 2, p. 104. DOI: https://doi.org/10.1002/jsfa.2740200210
Rankine, B. C. 1968. The influence of yeasts in determining the composition and quality of wines. Vitis, vol. 7, no. 1, p. 22-49. PMid:5777007
Romano, P., Suzzi, G. 1993. Sulfur dioxide and wine microorganism. In: Fleet G. H. (Ed.) Wine Microbiology and Biotechnology. Harwood Academic Publishers, Chur, Switzerland. pp. 373-393.
Suzzi, G., Romano, P., Zambonelli, C. 1985. Saccharomyces strain selection in minimizing SO2 requirement during vinification. American Journal of Enology and Viticulture, vol. 36, no. 1, p. 199-202.
Špakovská, E., Marcinčák, S., Bača, M., Turek, P. 2012. Polyphenolic content and antioxidative activiny of wines from the Sobrance wine region. Potravinarstvo, vol. 6, no. 3, p. 35-35. https://doi.org/10.5219/204 DOI: https://doi.org/10.5219/204
Wells, A., Osborne, J. P. 2011. Production of SO2 binding compounds and SO2 by Saccharomyces during alcoholic fermentation and the impact on malolactic fermentation. South African Journal of Enology and Viticulture, vol. 32, no. 2, p. 267-278. DOI: https://doi.org/10.21548/32-2-1387
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