Prevalence of pathogenic Yersinia enterocolitica in minced meat, pig tongues and hearts at the retail level in the Czech Republic detected by real time PCR

Authors

  • Alena Lorencova Veterinary Research Institute, The Department of Food and Feed Safety, Hudcova 70, 621 00 Brno, Czech Republic
  • Michal Slany Veterinary Research Institute, The Department of Food and Feed Safety, Hudcova 70, 621 00 Brno, Czech Republic

DOI:

https://doi.org/10.5219/616

Keywords:

Yersinia enterocolitica, ail gene, ompF gene, real time PCR, pork products, retail, zoonosis

Abstract

Yersiniosis is the third most frequently reported zoonosis in the European Union and Yersinia enterocolitica is the most common species causing human infections. Pigs are assumed to be the main reservoir of human pathogenic Y. enterocolitica with the presence of bacteria mainly in the tonsils and intestinal content. Undercooked pork and pork products have been suggested as the primary source of human yersiniosis. Nevertheless, data on the prevalence of pathogenic Y. enterocolitica in foodstuffs including pork products are very limited. A molecular based method (real time PCR) targeting the ompF gene (detection of Yersinia genus) and the ail gene (a chromosomally located virulence marker of Y. enterocolitica) was used to determine the prevalence of pathogenic Y. enterocolitica in minced meat and edible pork offal at the retail level in the Czech Republic. A total of 50 pig tongues, 50 pig hearts, and 93 samples of minced meat containing pork were purchased at nine retail outlets in Brno. High detection rates of Yersinia spp. were found in all types of samples (pig tongues, 80.0%; pig hearts, 40.0%; and minced meat, 55.9%). The highest prevalence of pathogenic Y. enterocolitica was found in pig tongues (40.0%), followed by pig hearts (18.0%) and minced meat samples (17.2%). Although from the point of view of food safety the merely molecular detection of DNA of the pathogenic bacteria could represent a false positive result, our results indicate the presence of pathogenic Y. enterocolitica in raw pork products at the retail level in the Czech Republic, which may pose a risk of consumer infection. Sufficient heat treatment and prevention of cross-contamination during preparation of food in the kitchen should be recommended.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Aldová, E., Švandová, E. 1984. Yersinia enterocolitica 03 findings on porcine tongues in comparison with yersiniosis incidence in man in Czechoslovakia. Journal of Hygiene, Epidemiology, Microbiology and Immunology, vol. 28, no. 3, p. 319-329. PMid:6491274

Aldová, E., Švandová, E., Votýpka, J., Šourek, J. 1990. Comparative-study of culture methods to detect Yersinia enterocolitica serogroup O3 on swine tongues. Zentralblatt für Bakteriologie, vol. 272, no. 3, p. 306-312. https://doi.org/10.1016/S0934-8840(11)80032-0 DOI: https://doi.org/10.1016/S0934-8840(11)80032-0

Boyapalle, S., Wesley, I. V., Hurd, H. S., Reddy, P. G. 2001. Comparison of culture, multiplex, and 5' nuclease polymerase chain reaction assays for the rapid detection of Yersinia enterocolitica in swine and pork products. Journal of Food Protection, vol. 64, no. 9, p. 306-312. DOI: https://doi.org/10.4315/0362-028X-64.9.1352

Bucher, M., Meyer, C., Grötzbach, B., Wacheck, S., Stolle, A., Fredriksson-Ahomaa, M. 2008. Epidemiological data on pathogenic Yersinia enterocolitica in Southern Germany during 2000-2006. Foodborne Pathogens and Disease, vol. 5, no. 3, p. 273-280. https://doi.org/10.1089/fpd.2007.0076 PMid:18564908 DOI: https://doi.org/10.1089/fpd.2007.0076

EFSA, 2011. EFSA panels on biological hazards (BIOHAZ), on contaminants in the food chain (CONTAM), and on animal health and welfare (AHAW). Scientific opinion on the public health hazards to be covered by inspection of meat (swine). EFSA Journal 2011; vol. 9, no. 10, 198 p. DOI: https://doi.org/10.2903/j.efsa.2011.2351

EFSA and ECDC. 2015. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2014. EFSA Journal, vol. 13, no. 12, 191 p. DOI: https://doi.org/10.2903/j.efsa.2015.4329

Fosse, J., Seegers, H., Magras, C. 2008. Foodborne zoonoses due to meat: a quantitative approach for a comparative risk assessment applied to pig slaughtering in Europe. Veterinary Research, vol. 39, no. 1, p. 1-16. https://doi.org/10.1051/vetres:2007039 PMid:18073088 DOI: https://doi.org/10.1051/vetres:2007039

Fredriksson-Ahomaa, M., Hielm, S., Korkeala, H. 1999. High prevalence of yadA-positive Yersinia enterocolitica in pig tongues and minced meat at the retail level in Finland. Journal of Food Protection, vol. 62, no. 2, p. 123-127. PMid:10030629 DOI: https://doi.org/10.4315/0362-028X-62.2.123

Fredriksson-Ahomaa, M., Korte, T., Korkeala, H. 2000. Contamination of carcasses, offals, and the environment with yadA-positive Yersinia enterocolitica in a pig slaughterhouse. Journal of Food Protection, vol. 63, no. 1, p. 31-35. PMid:10643766 DOI: https://doi.org/10.4315/0362-028X-63.1.31

Fredriksson-Ahomaa, M., Hallanvuo, S., Korte, T., Siitonen, A., Korkeala, H. 2001a. Correspondence of genotypes of sporadic Yersinia enterocolitica bioserotype 4/O:3 strains from human and porcine sources. Epidemiology and Infection, vol. 127, no. 1, p. 37-47. https://doi.org/10.1017/S0950268801005611 PMid:11561973 DOI: https://doi.org/10.1017/S0950268801005611

Fredriksson-Ahomaa, M., Bucher, M., Hank, C., Stolle, A., Korkeala, H. 2001b. High prevalence of Yersinia enterocolitica 4:O3 on pig offal in southern Germany: A slaughtering technique problem. Systematic and Applied Microbiology, vol. 24, no. 3, p. 457-463. https://doi.org/10.1078/0723-2020-00055 PMid:11822684 DOI: https://doi.org/10.1078/0723-2020-00055

Fredriksson-Ahomaa, M., Lyhs, U., Korte, T., Korkeala, H. 2001c. Prevalence of pathogenic Yersinia enterocolitica in food samples at retail level in Finland. Archiv für Lebensmittelhygiene, vol. 52, no. 3, p. 66-68.

Fredriksson-Ahomaa, M., Korkeala, H. 2003. Low occurrence of pathogenic Yersinia enterocolitica in clinical, food, and environmental samples: a methodological problem. Clinical Microbiology Reviews, vol. 16, no. 2, p. 220-229. https://doi.org/10.1128/CMR.16.2.220-229.2003 PMid:12692095 DOI: https://doi.org/10.1128/CMR.16.2.220-229.2003

Galindo, C. L., Rosenzweig, J. A., Kirtley, M. L., Chopra, A. K. 2011. Pathogenesis of Y. enterocolitica and Y. pseudotuberculosis in human yersiniosis. Journal of Pathogens, vol. 2011, p. 1-16. https://doi.org/10.4061/2011/182051 PMid:22567322 DOI: https://doi.org/10.4061/2011/182051

Grahek-Ogden, D., Schimmer, B., Cudjoe, K. S., Nygård, K., Kapperud, G. 2007. Outbreak of Yersinia enterocolitica serogroup O:9 infection and processed pork, Norway. Emerging Infectious Diseases, vol. 13, no. 5, p. 754-756. https://doi.org/10.3201/eid1305.061062 PMid:17553258 DOI: https://doi.org/10.3201/eid1305.061062

Lambertz, S. T., Granath, K., Fredriksson-Ahomaa, M., Johansson, K. E., Danielsson-Tham, M. L. 2007. Evaluation of a combined culture and PCR method (NMKL-163A) for detection of presumptive pathogenic Yersinia enterocolitica in pork products. Journal of Food Protection, vol. 70, no. 2, p. 335-340. PMid:17340866 DOI: https://doi.org/10.4315/0362-028X-70.2.335

Lambertz, S. T., Nilsson, C., Hallanvuo, S., Lindblad, M. 2008. Real-time PCR method for detection of pathogenic Yersinia enterocolitica in food. Applied and Environmental Microbiology, vol. 74, no. 19, p. 6060-6067. https://doi.org/10.1128/AEM.00405-08 PMid:18708521 DOI: https://doi.org/10.1128/AEM.00405-08

Laukkanen-Ninios, R., Fredriksson-Ahomaa, M., Maijala, R., Korkeala, H. 2014. High prevalence of pathogenic Yersinia enterocolitica in pig cheeks. Food Microbiology, vol. 43, p. 50-52. https://doi.org/10.1016/j.fm.2014.04.016 PMid:24929882 DOI: https://doi.org/10.1016/j.fm.2014.04.016

Messelhäusser, U., Kämpf, P., Colditz, J., Bauer, H., Schreiner, H., Höller, C., Busch, U. 2011. Qualitative and quantitative detection of human pathogenic Yersinia enterocolitica in different food matrices at retail level in Bavaria. Foodborne Pathogens and Disease, vol. 8, no. 1, p. 39-44. https://doi.org/10.1089/fpd.2010.0589 PMid:21034270 DOI: https://doi.org/10.1089/fpd.2010.0589

Miller, V. L., Farmer, J. J. 3rd, Hill, W. E., Falkow, S. 1989. The ail locus is found uniquely in Yersinia enterocolitica serotypes commonly associated with disease. Infection and Immunity, vol. 57, no. 1, p. 121-131. PMid:2642465 DOI: https://doi.org/10.1128/iai.57.1.121-131.1989

Simonova, J., Borilova, G., Steinhauserova, I. 2008. Occurrence of pathogenic strains of Yersinia enterocolitica in pigs and their antimicrobial resistance. Bulletin of the Veterinary Institute in Pulawy, vol. 52, no. 1, p. 39-43.

Slana, I., Kralik, P., Kralova, A., Pavlik, I. 2008. On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination. International Journal of Food Microbiology, vol. 128, no. 2, p. 250-257. https://doi.org/10.1016/j.ijfoodmicro.2008.08.013 PMid:18824269 DOI: https://doi.org/10.1016/j.ijfoodmicro.2008.08.013

Stenkova, A. M., Isaeva, M. P., Rasskazov, V. A. 2008. Development of a multiplex PCR procedure for detection of Yersinia genus with identification of pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica). Moleculer Genetics, Microbiology and Virology, vol. 23, no. 3, p. 119-125. https://doi.org/10.3103/S0891416808030038 DOI: https://doi.org/10.3103/S0891416808030038

Tauxe, R. V., Vandepitte, J., Wauters, G., Martin, S. M., Goossens, V., De Mol, P., Van Noyen, R., Thiers, G. 1987. Yersinia enterocolitica infections and pork - the missing link. Lancet, vol. 1, no. 8542, p. 1129-1132. https://doi.org/10.1016/S0140-6736(87)91683-7 DOI: https://doi.org/10.1016/S0140-6736(87)91683-7

Van Damme, I., Berkvens, D., Vanantwerpena, G., Baré, J., Houf, K., Wautersa, G., De Zutteral, L. 2015. Contamination of freshly slaughtered pig carcasses with enteropathogenic Yersinia spp.: Distribution, quantification and identification of risk factors. International Journal of Food Microbiology, vol. 204, p. 33-40. https://doi.org/10.1016/j.ijfoodmicro.2015.03.016 PMid:25835198 DOI: https://doi.org/10.1016/j.ijfoodmicro.2015.03.016

Vázlerová, M., Steinhauserová, I. 2006. The comparison of the methods for the identification of pathogenic serotypes and biotypes of Yersinia enterocolitica: Microbiological methods and PCR. Czech Journal of Food Sciences, vol. 24, no. 5, p. 217-222. DOI: https://doi.org/10.17221/3316-CJFS

Downloads

Published

2016-06-03

How to Cite

Lorencova, A. ., & Slany, M. . (2016). Prevalence of pathogenic Yersinia enterocolitica in minced meat, pig tongues and hearts at the retail level in the Czech Republic detected by real time PCR. Potravinarstvo Slovak Journal of Food Sciences, 10(1), 282–286. https://doi.org/10.5219/616