Effect of diet supplemented with propolis extract and probiotic additives on performance, carcass characteristics and meat composition of broiler chickens
DOI:
https://doi.org/10.5219/581Keywords:
performance, meat, chicken, propolis, probioticAbstract
The present research focused on the effects of propolis extract and probiotic preparation based on Lactobacillus fermentum (1 × 109 CFU per 1 g of bearing medium) on performance, carcass characteristics and meat composition of broiler chickens. The experiment was performed with 360 one day-old Ross 308 broiler chicks of mixed sex. The chicks were randomly allocated into 3 groups (n = 120 pcs chicks per group), namely, control (C) and experimental (E1, E2). Each group consisted of 3 replicated pens with 40 broiler chickens per pen. The experiment employed a randomized design, and dietary treatments were as follows: 1. basal diet with no supplementation as control (group C), 2. basal diet plus 400 mg propolis extract per 1 kg of feed mixture (group E1), 3. basal diet plus 3.3 g probiotic preparation added to drinking water (group E2). Besides, the groups were kept under the same conditions. Fattening period lasted for 42 days. Feed mixtures were produced without any antibiotic preparations and coccidiostats. As regards performance of broilers, all the investigated parameters were improved after addition of the supplements, especially after probiotic supplementation. However, neither propolis extract nor probiotic in diet of broiler chickens had any significant effect (p ≥0.05) on performance. Meat composition was evaluated as proximate composition (dry matter, crude protein, fat and ash), cholesterol content and energy value in the most valuable parts of chicken meat (breast and thigh muscles). The statistically significant results (p ≤0.05) were attained in fat, ash and cholesterol content, as well as energy value in both breast and thigh muscles after the propolis supplementation. To sum up, the present study demonstrated the promising potential of propolis extract and probiotic to enhance the performance, carcass characteristics and meat composition under conditions of the experiment with, however, statistical significance of results in a few parameters.
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Açikgöz, Z., Yücel, B., Altan, Ö. 2005. The effects of propolis supplementation on broiler performance and feed digestibility. Archiv Für Geflügelkunde, vol. 69, no. 3, p. 117-122.
Ahmed, S. T., Islam, M. M., Bostami, A. B. M. R., Mun, H., Kim, Y., Yang. C. 2015. Meat composition, fatty acid profile and oxidative stability of meat from broilers supplemented with pomegranate (Punica granatum L.) by-products. Food Chemistry, vol. 188, p. 481-488. https://doi.org/10.1016/j.foodchem.2015.04.140 DOI: https://doi.org/10.1016/j.foodchem.2015.04.140
Ahmed, S. T., Islam, M. M., Mun, H., Sim, H., Kim, Y., Yang, C. 2014. Effects of Bacillus amyloliquefaciens as a probiotic strain on growth performance, cecal microflora, and fecal noxious gas emissions of broiler chickens. Poultry Science, vol. 93, no. 8, p. 1963-1971. https://doi.org/10.3382/ps.2013-03718 DOI: https://doi.org/10.3382/ps.2013-03718
Aliakbarpour, H. R., Chamani, M., Rahimi, G., Sadeghi, A. A., Qujeq, D. 2012. The Bacillus subtilis and Lactic Acid Bacteria Probiotics Influences Intestinal Mucin Gene Expression, Histomorphology and Growth Performance in Broilers. Asian-Australasian Journal of Animal Sciences, vol. 25, no. 9, p. 1285-1293. https://doi.org/10.5713/ajas.2012.12110 DOI: https://doi.org/10.5713/ajas.2012.12110
Alkhalf, A., Alhaj, M., Al-Homidan, I. 2010. Influence of probiotic supplementation on blood parameters and growth performance in broiler chickens. Saudi Journal of Biological Sciences, vol. 17, no. 3, p. 219-225. https://doi.org/10.1016/j.sjbs.2010.04.005 DOI: https://doi.org/10.1016/j.sjbs.2010.04.005
Apata, D. F. 2008. Growth performance, nutrient digestibility and immune response of broiler chicks fed diets supplemented with a culture of Lactobacillus bulgaricus. Journal of the Science of Food and Agriculture, vol. 88, no. 7, p. 1253-1258. https://doi.org/10.1002/jsfa.3214 DOI: https://doi.org/10.1002/jsfa.3214
Attia, Y. A., Abd Al-Hamid, A. E., Ibrahim, M. S., Al-Harthi, M. A., Bovera, F., Elnaggar, A. S. 2014. Productive performance, biochemical and hematological traits of broiler chickens supplemented with propolis, bee pollen, and mannan oligosaccharides continuously or intermittently. Livestock Science, vol. 164, p. 87-95. https://doi.org/10.1016/j.livsci.2014.03.005 DOI: https://doi.org/10.1016/j.livsci.2014.03.005
Bai, S. P., Wu, A. M., Sing, X. M., Lei, Y., Bai, J., Zhang, K. Y., Chio, J. S. 2013. Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens. Poultry Science, vol. 92, no. 3, p. 663-670. https://doi.org/10.3382/ps.2012-02813 DOI: https://doi.org/10.3382/ps.2012-02813
Biavatti, M. W., Bellaver, M. H., Volpato, L., Costa, C., Bellaver, C. 2003. Preliminary studies of alternative feed additives for broilers: Alternanthera brasiliana extract, propolis extract and linseed oil. Brazilian Journal of Poultry Science, vol. 5, no. 2, p. 147-151. https://doi.org/10.1590/s1516-635x2003000200009 DOI: https://doi.org/10.1590/S1516-635X2003000200009
Brzóska, F. A., Śliwiński, B. A., Stecka, K. B. 2012. Effect of Lactococcus lactis vs. Lactobacillus spp. bacteria on chicken body weight, mortality, feed conversion and carcass quality. Annals of Animal Science, vol. 12, no. 4, p. 549-559. https://doi.org/10.2478/v10220-012-0046-y DOI: https://doi.org/10.2478/v10220-012-0046-y
Daneshmand, A., Sadeghi, G. H., Karimi, A., Vaziry, A., Ibrahim, S. A. 2015. Evaluating complementary effects of ethanol extract of propolis with the probiotic on growth performance, immune response and serum metabolites in male broiler chickens. Livestock Science, vol. 178, p. 195-201. https://doi.org/10.1016/j.livsci.2015.04.012 DOI: https://doi.org/10.1016/j.livsci.2015.04.012
Fan, Y., Lu, Y., Wang, D., Liu, J., Song, X., Zhang, W., Zhao, X., Nguyen, L., Hu, Y. 2013. Effect of epimedium polysaccharide-propolis flavone immunopotentiator on immunosuppression induced by cyclophosphamide in chickens. Cellular Immunology, vol. 281, no. 1, p. 37-43. https://doi.org/10.1016/j.cellimm.2013.01.008 DOI: https://doi.org/10.1016/j.cellimm.2013.01.008
Ghahri, H., Toloei, T., Soleimani, B. 2013. Efficacy of Antibiotic, Probiotic, Prebiotic and Synbiotic on Growth Performance, Organ Weights, Intestinal Histomorphology and Immune Response in Broiler Chickens. Global Journal of Animal Scientific Research, vol. 1, no. 1, p. 25-41.
Ghasemi, H. A., Kasani, N., Taherpour, K. 2014. Effects of black cumin seed (Nigella sativa L.), a probiotic, a prebiotic and a synbiotic on growth performance, immune response and blood characteristics of male broilers. Livestock Science, vol. 164, p. 128-134. https://doi.org/10.1016/j.livsci.2014.03.014 DOI: https://doi.org/10.1016/j.livsci.2014.03.014
Giannenas, I., Papadopoulos, E., Tsalie, E., Triantafillou, E., Henikl, S., Teichmann, K., Tontis, D. 2012. Assessment of dietary supplementation with probiotics on performance, intestinal morphology and microflora of chickens infected with Eimeria tenella. Veterinary Parasitology, vol. 188, no. 1-2, p. 31-40. https://doi.org/10.1016/j.vetpar.2012.02.017 DOI: https://doi.org/10.1016/j.vetpar.2012.02.017
Goodarzi, M., Nanekarani, S. 2014. Effect of Onion Extract in Drink Water on Performance and Carcass Traits in Broiler Chickens. IERI Procedia 8: International Conference on Agricultural and Biosystem Engineering, p. 107-112. https://doi.org/10.1016/j.ieri.2014.09.018 DOI: https://doi.org/10.1016/j.ieri.2014.09.018
Goodarzi, M., Nanekarani, S., Landy, N. 2014. Effect of dietary supplementation with onion (Allium cepa L.) on performance, carcass traits and intestinal microflora composition in broiler chickens. Asian Pacific Journal of Tropical Disease, vol. 4, p. S297-S301. https://doi.org/10.1016/s2222-1808(14)60459-x DOI: https://doi.org/10.1016/S2222-1808(14)60459-X
Hashemi, S. R., Zulkifli, I., Davoodi, H., Zunita, Z., Ebrahimi, M. 2012. Growth performance, intestinal microflora, plasma fatty acid profile in broiler chickens fed herbal plant (Euphorbia hirta) and mix of acidifiers. Animal Feed Science and Technology, vol. 178, no. 3-4, p. 167-174. https://doi.org/10.1016/j.anifeedsci.2012.09.006 DOI: https://doi.org/10.1016/j.anifeedsci.2012.09.006
Haščík, P., Elimam, I. O. E., Garlík, J., Kačániová, M., Bobko, M., Kňazovická, V., Vavrišinová, K., Arpášová, H., Bučko, O. 2012. Chemical composition of muscle after pollen application in nutrition of broiler chickens. Potravinarstvo, vol. 6, no. 2, p. 26-32. https://doi.org/10.5219/190 DOI: https://doi.org/10.5219/190
Haščík, P., Kačániová, M., Bobko, M., Pochop, J., Mihok, M., Arpášová, H. 2011. Effect of probiotic preparation for chemical composition of meat cocks different combinations of hybrid chicks. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, vol. 59, no. 5, p. 83-93. https://doi.org/10.11118/actaun201159050083 DOI: https://doi.org/10.11118/actaun201159050083
Haščík, P., Kročko, M., Garlík, J., Elimam, I., Kačániová, M., Bobko, M., Arpášová, H., Vavrišinová, K., Bučko, O. 2014. The effect of propolis extract in the diet of chickens Ross 308 on their performance. Journal of Central European Agriculture, vol. 15, no. 4, p. 133-146. https://doi.org/10.5513/jcea01/15.4.1521 DOI: https://doi.org/10.5513/JCEA01/15.4.1521
Horňáková, M., Chromý, V., Heyrovský, A. 1974. Spektrofotometrické stanovení cholesterolu. Biochem. Clin. Bohem., vol. 3, p. 27-34.
Hossain, M. E., Yang, C. J. 2014. Effect of fermented water plantain on growth performance, meat composition, oxidative stability, and fatty acid composition of broiler. Livestock Science, vol. 162, p. 168-177. https://doi.org/10.1016/j.livsci.2014.01.016 DOI: https://doi.org/10.1016/j.livsci.2014.01.016
Kabir, S. M. L., Rahman, M. M., Rahman, M. B., Rahman, M. M., Ahmed, S. U. 2004. The Dynamics of Probiotics on Growth Performance and Immune Response in Broilers. International Journal of Poultry Science, vol. 3, no. 5, p. 361-364. https://doi.org/10.3923/ijps.2004.361.364 DOI: https://doi.org/10.3923/ijps.2004.361.364
Khaksefidi, A., Rahimi, S. H. 2005. Effect of Probiotic Inclusion in the Diet of Broiler Chickens on Performance, Feed Efficiency and Carcass Quality. Asian-Australasian Journal of Animal Sciences, vol. 18, no. 8, p. 1153-1156. https://doi.org/10.5713/ajas.2005.1153 DOI: https://doi.org/10.5713/ajas.2005.1153
Krell, R. 1996. Value-added products from bee keeping. FAO. Rome (IT). 395 p.
Naseem, S., Rahman, S. U., Shafee, M., Sheikh, A. A., Khan, A. 2012. Immunomodulatory and Growth-Promoting Effect of a Probiotic Supplemented in the Feed of Broiler Chicks Vaccinated Against Infectious Bursal Disease. Brazilian Journal of Poultry Science, vol. 14, no. 2, p. 109-113. https://doi.org/10.1590/s1516-635x2012000200004 DOI: https://doi.org/10.1590/S1516-635X2012000200004
Petrová, J., Terentjeva, M., Puchalski, C., Hutková, J., Kántor, A., Mellen, M., Čuboň, J., Haščík, P., Kluz, M., Kordiaka, R., Kunová, S., Kačániová, M. 2015. Application of lavender and rosemary essential oils improvement of the microbiological quality of chicken quarters. Potravinarstvo, vol. 9, no. 2, p. 530-537. https://doi.org/10.5219/550 DOI: https://doi.org/10.5219/550
Ritzi, M. M., Abdelrahman, W., Mohnl, M., Dalloul, R. A. 2014. Effects of probiotics and application methods on performance and response of broiler chickens to an Eimeria challenge. Poultry Science, vol. 93, no. 11, p. 2772-2778. https://doi.org/10.3382/ps.2014-04207 DOI: https://doi.org/10.3382/ps.2014-04207
Seven, I., Aksu, T., Tatli Seven, P. 2012. The effects of propolis and vitamin C supplemented feed on performance, nutrient utilization and carcass characteristics in broilers exposed to lead. Livestock Science, vol. 148, no. 1-2, p. 10-15. https://doi.org/10.1016/j.livsci.2012.05.001 DOI: https://doi.org/10.1016/j.livsci.2012.05.001
Shalmany, S. K., Shivazad, M. 2006. The effect of diet propolis supplementation on Ross broiler chicks performance. International Journal of Poultry Science, vol. 5, no. 1, p. 84-88. https://doi.org/10.3923/ijps.2006.84.88 DOI: https://doi.org/10.3923/ijps.2006.84.88
Skřivan, M., Marounek, M., Englmaierová, M., Skřivanová, E. 2012. Influence of dietary vitamin C and selenium, alone and in combination, on the composition and oxidative stability of meat of broilers. Food Chemistry, vol. 130, no. 3, p. 660-664. https://doi.org/10.1016/j.foodchem.2011.07.103 DOI: https://doi.org/10.1016/j.foodchem.2011.07.103
Strmiska, F., Holčíková, K., Simonová, E., Mrázová, M., Hodeková, J., Vojtaššáková, A., Pristašová. M., Strmiska, J., Strmisková, G., Krupařová, M., Gola, J., Papajová, H., Mareš, J., Kostkanová, E., Ehrenhaft, B. 1988. Foodstuff tables for food materials (in Slovak). Výskumný ústav potravinársky, Bratislava (SK), p. 10-12.
Swiatkiewicz, S., Arczewska-Wlosek, A., Jozefiak, D. 2014. Feed enzymes, probiotic, or chitosan can improve the nutritional efficacy of broiler chicken diets containing a high level of distillers dried grains with soluble. Livestock Science, vol. 163, p. 110-119. https://doi.org/10.1016/j.livsci.2014.03.001 DOI: https://doi.org/10.1016/j.livsci.2014.03.001
Taklimi, S. M. S. M., Lotfollahian, H., Shahne, A. Z., Mirzaei, F., Alinejad, A. 2012. Study on efficacy of probiotic in broiler chickens diet. Agricultural Sciences, vol. 3, no. 1, p. 5-8. https://doi.org/10.4236/as.2012.31002 DOI: https://doi.org/10.4236/as.2012.31002
Tatlı Seven, P., Seven, I., Yilmaz, M., Şimşek, G. Ü. 2008. The effect of Turkish propolis on growth and carcass characteristics in broilers under heat stress. Animal Feed Science and Technology, vol. 146, no. 1-2, p. 137-148. https://doi.org/10.1016/j.anifeedsci.2007.11.003 DOI: https://doi.org/10.1016/j.anifeedsci.2007.11.003
Umaya Suganthi, R. 2014. The Uniqueness of Immunocompetence and Meat Quality of Native Chickens: A Specialized Review. World Journal of Pharmacy and Pharmaceutical Sciences, vol. 3, no. 2, p. 2576-2588.
Valenzuela-Barra, G., Castro, C., Figueroa, C., Barriga, A., Silva, X., De Las Heras, B., Hortelano, S., Delporte, C. 2015. Anti-inflammatory activity and phenolic profile of propolis from two locations in Región Metropolitana de Santiago, Chile. Journal of Ethnopharmacology, vol. 168, p. 37-44. https://doi.org/10.1016/j.jep.2015.03.050 DOI: https://doi.org/10.1016/j.jep.2015.03.050
Ziaran, H. R., Rahmani, H. R., Pourreza, J. 2005. Effect of dietary oil extract of propolis on immune response and broiler performance. Pakistan Journal of Biological Sciences, vol. 8, no. 10, p. 1485-1490. https://doi.org/10.3923/pjbs.2005.1485.1490 DOI: https://doi.org/10.3923/pjbs.2005.1485.1490
Zulkifli, I., Abdullah, N., Azrin, N. M., Ho, Y. W. 2000. Growth performance and immune response of two commercial broiler strains fed diets containing Lactobacillus cultures and oxytetracycline under heat stress conditions. British Poultry Science, vol. 41, no. 5, p. 593-597. https://doi.org/10.1080/713654979 DOI: https://doi.org/10.1080/713654979
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