Methods for fish species identification in food products

Pavol  Bajzí­k; Jozef  Golian; Radoslav  Židek; Jozef  Čapla; Ľubomí­r  Belej; Matúš  Ondrejka; Ľubica  Mrázová; Lenka  Maršálková

doi:10.5219/25

Authors

Pavol Bajzík Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, EU
Jozef Golian Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, EU
Radoslav Židek Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, EU
Jozef Čapla Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, EU
Ľubomír Belej Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, EU
Matúš Ondrejka Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, EUia
Ľubica Mrázová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, EU
Lenka Maršálková Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia, EU

DOI:

https://doi.org/10.5219/25

Keywords:

PCR sequencing, multiplex PCR, PCR-RFLP, PCR-SSCP, RAPD, real-time PCR

Abstract

The need for identification of fishery products in food is currently ongoing issue for both consumers and producers of food. Consumer interest is driven in one the healthy diet, which prefers fish products, as an indispensable ingredient food and on the other hand, is a potential allergen causing health problems in humans allergic to fish protein. Allergy is a phenomenon that significantly affects human health, as well as overall life expectancy of an individual. The large number of fish species are known to trigger allergic reactions directly food intake or inhalation of fumes only, depending on the sensitivity orgamizmu. Large quantity of fish allergens are proteins from the stock protein to enzymes. Methods used for species identifications of fish in food products are PCR sequencing, multiplex PCR, PCR-RFLP, PCR-SSCP, RAPD, real-time PCR.

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References

ARAHISHI, F. 2005. PCR-RFLP analysis of nuclear nontranscribed spacer for mackerel species identification. In Journal of Agricultural and Food Chemistry, vol. 53, 2005, p. 508- 511.

https://doi.org/10.1021/jf0484881 DOI: https://doi.org/10.1021/jf0484881

PMid:15686394

ASENSIO, L., GONZÁLEZ, I., FERNÁNDEZ, A., CÉSPEDE, A. RODRÍGUEZ, M. A., HERNÁNDEZ, P. E. 2001. Identification of Nile perch (Lates niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) fillets by PCR amplification of the 5S rDNA gene. In Journal of the AOAC International, vol. 84, 2001, p. 777-781. DOI: https://doi.org/10.1093/jaoac/84.3.777

ATAMAN, C., CELIK, U., REHBEIN, H. 2006. Identification of some Aegean fish species by native isoelectric focusing. In European Food Research and Technology, vol. 222, 2006, p. 99-104.

https://doi.org/10.1007/s00217-005-0149-0 DOI: https://doi.org/10.1007/s00217-005-0149-0

BARDAKCI, F., SKIBINSKI, D. O. F. 1994. Application of the RAPD technique in tilapia fish: species and subspecies identification. In Heredity, vol. 73, 1994, p. 117-123.

https://doi.org/10.1038/hdy.1994.110 DOI: https://doi.org/10.1038/hdy.1994.110

PMid:8071079

CANAPA, A., BARUCCA, M., MARINEL, L. A., OLMO, E. 2000. Molecular data from the 16S rRNA gene for the phylogeny of Pectinidae (Mollusca: Bivalvia). In Journal of Molecular Evolution, vol. 50, 2000, p. 93-97. DOI: https://doi.org/10.1007/s002399910010

PMid:10654263

CALLEJAS, C., OCHANDO, M. D. 2001. Molecular identification (RAPD) of the eight species of the genus Barbus (Cyprinidae) in the Iberian Peninsula. In Journal of Fish Biology, vol. 59, 2001, p. 1589-1599.

https://doi.org/10.1111/j.1095-8649.2001.tb00223.x DOI: https://doi.org/10.1111/j.1095-8649.2001.tb00223.x

DAHLE, G., RAHMAN, M., ERIKSEN, A. G. 1997. RAPD fingerprinting used for discriminating among three populations of Hilsa shad (Tenualosa ilisha). In Fisheries Science, vol. 32, 1997, p. 263-269. DOI: https://doi.org/10.1016/S0165-7836(97)00058-1

FERNÁNDEZ, A., GARCÍA, T., ASENSIO, L., RODRÍGUEZ, M. A., GONZÁLEZ, I., LOBO, E. 2002. Identification of the clam species Ruditapes decussatus (grooved carpet shell), Venerupis romboides (yellow carpet shell) and Venerupis pullastra (pullet carpet shell) by ELISA. Food and Agricultural Immunology, vol. 14, 2002, p. 65-71. DOI: https://doi.org/10.1080/09540100220137673

FUJITA, K., SILVER, J. 1994. Single-strand conformational polymorphism. In PCR Methods and Applications, vol. 4, 1994, p. 137-139. DOI: https://doi.org/10.1101/gr.4.3.S137

HEID, C. A., STEVENS, J., LIVAK, K. J., WILLIAMS, P. M. 1996. Real-time quantitative PCR. In Genome Research, vol. 6, 1996, p. 986-994.

https://doi.org/10.1101/gr.6.10.986 DOI: https://doi.org/10.1101/gr.6.10.986

PMid:8908518

HIRD, H. J., HOLD, G. L., CHRISHOLM, J., REECE, P., RUSSELL, V. J., BROWN, J. 2005. Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR. In European Food Research and Technology, vol. 220, 2005, p. 633-637.

https://doi.org/10.1007/s00217-004-1050-y DOI: https://doi.org/10.1007/s00217-004-1050-y

HOLLAND, P. M., ABRAMSON, R. D., WATSON, R., GELFAND, D. H. 1991. Detection of specific polymerase chain reaction product by utilizing the 5´-3´ exonuclease activity of Thermus aquaticus DNA polymerase. In Proceedings of the National Academy of Sciences of the United States of America, vol. 88, 1991, p. 7276-7280.

https://doi.org/10.1073/pnas.88.16.7276 DOI: https://doi.org/10.1073/pnas.88.16.7276

HORSTKOTTE, B., REHBEIN, H. 2003. Fish species identification by means of restriction fragment length polymorphism and high performance liquid chromatography. In Journal of Food Science, vol. 68, 2003, p. 2658-2666.

https://doi.org/10.1111/j.1365-2621.2003.tb05785.x DOI: https://doi.org/10.1111/j.1365-2621.2003.tb05785.x

HSIEH, H. S., CHAI, T., HWANG, D. F. 2007. Using the PCReRFLP method to identify the species of different processed products of billfish meats. In Food Control, vol. 18, 2007, p. 369-374.

https://doi.org/10.1016/j.foodcont.2005.11.002 DOI: https://doi.org/10.1016/j.foodcont.2005.11.002

HUBALKOVÁ, Z., KRÁLIK, P., KASALOVÁ, J., RENČOVÁ, E. 2008. Identification of gadoid species in fish meat by polymerase chain reaction (PCR) on genomic DNA. In J Agric Food Chem., vol. 56, 2008, no. 10, p. 3454-9. DOI: https://doi.org/10.1021/jf703684w

JÉRÔME, M., LEMAIRE, C., BAUTISTA, J. M., FLEURENCE, J., ETIENNE, M. 2003. Molecular phylogeny and species identification of sardines. In Journal of Agricultural and Food Chemistry, vol. 51, 2003, p. 43-50. DOI: https://doi.org/10.1021/jf020713w

JIN, L. G., CHO, J. G., SEONG, K. B., PARK, J. Y., KONG, I. S., HONG, Y. K. 2006. 18 rRNA gene sequences and random amplified polymorphic DNA used in discriminating Manchurian trout from other freshwater salmonids. In Fisheries Science, vol. 72, 2006, p. 903-905.

https://doi.org/10.1111/j.1444-2906.2006.01234.x DOI: https://doi.org/10.1111/j.1444-2906.2006.01234.x

JONKER, K. M., TILBURG, J. J., HAGELE, G. H., DE BOER, E. 2008. Species identification in meat products using real-time PCR. In Food Addit Contam Part A Chem Anal Control Expo Risk Assess., vol. 25, 2008, no. 5, p. 527-533. DOI: https://doi.org/10.1080/02652030701584041

KNUUTINEN, J., HARJULA, P. 1998. Identification of fish species by reverse-phase high-performance liquid chromatography with photodiode-array detection. In Journal of Chromatography B, vol. 705, 1998, p.11-21.

https://doi.org/10.1016/S0378-4347(97)00505-7 DOI: https://doi.org/10.1016/S0378-4347(97)00505-7

KOCHZIUS M., NÖLTE M., WEBER H., SILKENBEUMER N., HJÖRLEIFSDOTTIR S., HREGGVIDSSON G.O, MARTEINSSON V., KAPPEL K., PLANES S., TINTI F., MAGOULAS A., GARCIA VAZQUEZ E., TURAN C., HERVET C., CAMPO FALGUERAS D., ANTONIOU A., LANDI M., BLOHM D. 2008. DNA microarrays for identifying fishes. In Mar Biotechnol (NY), vol.10, 2008, no. 2, p.207-17. DOI: https://doi.org/10.1007/s10126-007-9068-3

LIN, W. F., HWANG, D. F. 2007. Application of PCR-RFLP analysis on species identification of canned tuna. In Food Control, vol. 18, 2007, p. 1050-1057.

https://doi.org/10.1016/j.foodcont.2006.07.001 DOI: https://doi.org/10.1016/j.foodcont.2006.07.001

LIN, Y. S., POH, Y. P., TZENG, C. S. 2001. A phylogeny of freshwater eels inferred from mitochondrial genes. In Molecular Phylogenetics and Evolution, vol. 20, 2001, p. 252- 261.

https://doi.org/10.1006/mpev.2001.0969 DOI: https://doi.org/10.1006/mpev.2001.0969

PMid:11476633

LOCKLEY, A. K., BARDSLEY, R. G. 2000. DNA-based methods for food authentication. In Trends in Food Science and Technology, vol. 11, 2000, p. 67-77.

https://doi.org/10.1016/S0924-2244(00)00049-2 DOI: https://doi.org/10.1016/S0924-2244(00)00049-2

LÓPEZ, I., PARDO, M. A. 2005. Application of relative quantification Taqman real-time polymerase chain reaction technology for theidentification and quantification of Thunnus alalunga and Thunnus albacares. In Journal of Agricultural and Food Chemistry, vol. 53, 2005, p. 4554-4560.

https://doi.org/10.1021/jf0500841 DOI: https://doi.org/10.1021/jf0500841

PMid:15913324

MACKIE, I., CRAIG, A., ETIENNE, M., JEROME, M., FLEURENCE, J., JESSEN, F. 2000. Species identification of smoked and gravid fish products by sodium dodecylsulphate polyacrylamide gel electrophoresis, urea isoelectric focusing and native isoelectric focusing: a collaborative study.In Food Chemistry, vol. 71, 2000, p. 1-7.

https://doi.org/10.1016/S0308-8146(00)00147-3 DOI: https://doi.org/10.1016/S0308-8146(00)00147-3

MACKIE, I. M., PRYDE, S. E., GONZALES-SOTELO, C., MEDINA, I., PÉREZMARTÍN, R., QUINTEIRO, J. 1999. Challenges in the identification of species of canned fish. In Trends in Food Science and Technology, 1999, p. 9-14. DOI: https://doi.org/10.1016/S0924-2244(99)00013-8

MACKIE, I. M. 1996. Authenticity of fish. P. R. Ashurt, M. J. Dennis (Eds.), In Food authentication, London: Blackie Academic and Professional, 1996, p. 140-170. DOI: https://doi.org/10.1007/978-1-4613-1119-5_5

MARKO, P. B., LEE, S. C., RIC, A. M., GRAMLING, J. M., FITZHENRY, T. M., MCAALISTER, J. S. 2004. Mislabelling of a depleted reef fish.In Nature, vol. 430, 2004, p. 309-310.

https://doi.org/10.1038/430309b DOI: https://doi.org/10.1038/430309b

PMid:15254528

MARTÍNEZ, I., MALMHEDEN YMAN, I. 1998. Species identification in meat products by RAPD analysis. In Food Research International, vol. 31, 1998, p. 459-466.

https://doi.org/10.1016/S0963-9969(99)00013-7 DOI: https://doi.org/10.1016/S0963-9969(99)00013-7

MEYER, R., HOFELEIN, C., LUTHY, J., CANDRIAN, U. 1995. Polymerase chain reaction-restriction fragment length polymorphism analysis: a simple method for species identification in food. In Journal of the AOAC International, vol. 78, 1995, p. 1542-1551. DOI: https://doi.org/10.1093/jaoac/78.6.1542

PMid:8664595

MURGIA, R., TOLA, G., ARCHER, S. N., VALLERGA, S., HIRANO, J. 2002. Genetic identification of grey mullet species (Mugilidae) by analysis of mitochondrial DNA sequence: application to identify the origin of processed ovary products (Bottarga). In Marine Biotechnology, vol. 4, 2002, p. 119-126.

https://doi.org/10.1007/s10126-001-0074-6 DOI: https://doi.org/10.1007/s10126-001-0074-6

PMid:14961270

OCHIAI, Y., OCHIAI, L., HASHIMOTO, K., WATABE, S. 2001. Quantitative estimation of dark muscle content in the mackerel meat paste and its products using antisera against myosin light chains. In Journal of Food Science, vol. 66, 2001, p. 1301-1305.

https://doi.org/10.1111/j.1365-2621.2001.tb15205.x DOI: https://doi.org/10.1111/j.1365-2621.2001.tb15205.x

OOHARA, I. 1997. Detection of single strand conformation polymorphisms (SSCPs) on mitochondrial DNA fragments between two domesticated strains of rainbow trout Oncorhynchus mykiss. Fisheries Science, vol. 63, 1997, p. 151-152. DOI: https://doi.org/10.2331/fishsci.63.151

PARDO, M. A., PÉREZ-VILLAREAL, B. 2004. Identification of commercial canned tuna species by restriction site analysis of mitochondrial DNA products obtained by nested primer PCR. In Food Chemistry, vol. 86, 2004, p.143-150.

https://doi.org/10.1016/j.foodchem.2003.09.024 DOI: https://doi.org/10.1016/j.foodchem.2003.09.024

PARTIS, L., WELLS, R. J. 1996. Identification of fish species using random amplified polymorphic DNA (RAPD). In Molecular and Cellular Probes, vol. 10, 1996, p. 435-441.

https://doi.org/10.1006/mcpr.1996.0060 DOI: https://doi.org/10.1006/mcpr.1996.0060

PMid:9025081

PEPE, T., TROTTA, M., DIMARCO, I., CENNAMO, P., ANASTASIO, A., CORTESI, M. L. 2005. Mitochondrial cytochrom b DNA sequence variations: an approach to fish species identification in processed fish products. In Journal of Food Protection, vol. 68, 2005, p. 421-425. DOI: https://doi.org/10.4315/0362-028X-68.2.421

RAM, J. L., RAM, M. L., BAIDOUN, F. F. 1996. Authentication of canned tuna and bonito sequence and restriction site analysis of polymerase chain reaction products of mitochondrial DNA. In Journal of Agricultural and Food Chemistry, vol. 44, 1996, p. 2460- 2467.

https://doi.org/10.1021/jf950822t DOI: https://doi.org/10.1021/jf950822t

REHBEIN, H., SOTELO, C. G., PÉREZ-MARTÍN, R. I. CHAPELA-GARRIDO, M. J., HOLD, G. L., RUSSELL V. J. 2002. Differentiation of raw or processed eel by PCR-based techniques: restriction fragment length polymorphism analysis (RFLP) and single strand conformation polymorphism analysis (SSCP). In European Food Research and Technology, vol. 214, 2002, p. 171-177.

https://doi.org/10.1007/s00217-001-0457-y DOI: https://doi.org/10.1007/s00217-001-0457-y

REHBEIN, H., KRESS, G., SCHMIDT, T. 1997. Application of PCR-SSCP to species identification of fishery products. In Journal of Science of Food and Agriculture, vol. 74, 1997, p. 35-41.

https://doi.org/10.1002/(SICI)1097-0010(199705)74:1<35::AID-JSFA765>3.0.CO;2-2 DOI: https://doi.org/10.1002/(SICI)1097-0010(199705)74:1<35::AID-JSFA765>3.0.CO;2-2

REHBEIN, H., MACKIE, I. M., PRYDE, S., GONZÁLES-SOTELO, C., MEDINA, I., PÉREZ-MARTÍN, R. 1999. Fish species identification in canned tuna by PCR-SSCP: validation by a collaborative study and investigation of intra-species variability of the DNApatterns. In Food Chemistry, vol. 64, 1999, p. 263-268.

https://doi.org/10.1016/S0308-8146(98)00125-3 DOI: https://doi.org/10.1016/S0308-8146(98)00125-3

SEBASTIO, P., ZANELLI, P., NERI-TAURO, M. 2001. Identification of anchovy (Engraulis encrasicholus L.) and gilt sardine (Sardinella aurita) by polymerase chain reaction, sequence of their mitochondrial cytochrome b gene, and restriction analysis of polymerase chain reaction products in semipreserves. In Journal of Agricultural and Food Chemistry, vol. 49, 2001, p. 1194-1199.

https://doi.org/10.1021/jf000875x DOI: https://doi.org/10.1021/jf000875x

PMid:11312834

ŠMARDA, J., DOŠKAR, J., PANTUČE, R., RUŽIČKOVÁ, V., KOPÍTKOVÁ, J. 2005. Metody molekulární biologie, 1.vyd. Masarykova univerzita v Brne, 2005, ISBN 80-210-3841-1.

VENKATESH, B., BRENNER, S. 1997. Genomic structure and sequence of the pufferfish (Fugu rubripes) growth hormone-encoding gene, a comparative analysis of teleost growth hormone gene. In Gene, vol. 187, 1997, p. 211-215.

https://doi.org/10.1016/S0378-1119(96)00750-0 DOI: https://doi.org/10.1016/S0378-1119(96)00750-0

WEDER, J. K. P., REHBEIN, H., KAISER, K. P. 2001. On the specificity of tunadirected primers in PCR-SSCP analysis of fish and meat. In Eur Food Res Technol., vol. 213, 2001, p. 139-144.

https://doi.org/10.1007/s002170100339 DOI: https://doi.org/10.1007/s002170100339