Ascorbic acid supplementation suppresses cadmium-derived alterations in the fission yeast Schizosaccharomyces pombe

Authors

  • Marek Kovár Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Plant Physiology, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414440 https://orcid.org/0000-0002-1478-8383
  • Alica Navrátilová Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Breeding Biology, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414296 https://orcid.org/0000-0002-8447-5016
  • Anna Trakovická Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Department of Genetics and Breeding Biology, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414285 https://orcid.org/0000-0002-6708-2384
  • Miroslava Požgajová Slovak University of Agriculture, AgroBioTech Research Center, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421376414919 https://orcid.org/0000-0003-3713-0717

DOI:

https://doi.org/10.5219/1618

Keywords:

cell, cadmium, ascorbic acid, oxidative stress, contamination

Abstract

Cadmium (Cd) a highly toxic environmental pollutant, that does not have any physiological function in the organism, represents a great concern for human health as it can be easily transported from its environmental sources to the food chain. Food, water, and air are the major sources of Cd exposure to the population. Cd-mediated impairments of the basic cellular properties largely depend on its ability to enhance the formation of reactive oxygen species (ROS) and thus triggers oxidative stress to the cell. With the use of fission yeast Schizosaccharomyces pombe (S. pombe) as the model organism, we have analyzed the impact of Cd on the cell growth intensity, as it represents the fundamental feature of all living organisms. Cells were incubated with different Cd concentrations for 3, 6, and 9 hours to investigate the effect of Cd on cell growth in a time and dose-dependent manner. Further possible Cd-derived alterations, as the peroxidation of membrane lipids or the functional impairment of the enzymatic antioxidant protection mechanisms, were investigated by determination of the MDA content and via catalase (CAT) activity detection. Moreover, ascorbic acid (AsA) pre-treatment was subjected to investigate the assumed positive effect of AsA against Cd toxicity. We show here on one hand that cells suffer under the influence of Cd, but on the other hand, they substantially profit from AsA supplementation. Because S. pombe is known to shares many molecular, and biochemical similarities with higher organisms, the effect of AsA in cadmium toxicity elimination might be expected to a similar extent also in other cell types.

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Published

2021-05-28

How to Cite

Kovár, M., Navrátilová, A., Trakovická, A., & Požgajová, M. (2021). Ascorbic acid supplementation suppresses cadmium-derived alterations in the fission yeast Schizosaccharomyces pombe. Potravinarstvo Slovak Journal of Food Sciences, 15, 423–432. https://doi.org/10.5219/1618