Detection of lasalocid residues in the tissues of broiler chickens by a new screening test total antibiotics

Ivona  Kožárová; Jana  Šimková; Mária  Mártonová; Ján  Mačanga; Martin  Levkut, ml.

doi:10.5219/140

Authors

Ivona Kožárová Department of Food Hygiene and Technology, Institute of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice
Jana Šimková Department of Chemistry, Biochemistry and Biophysics, Institute of Biochemistry, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice
Mária Mártonová Department of Chemistry, Biochemistry and Biophysics, Institute of Biochemistry, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice
Ján Mačanga Department of Food Hygiene and Technology, Institute of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice
Martin Levkut, ml. Department of Pathological Anatomy and Pathological Physiology, Institute of Pathological Physiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice

DOI:

https://doi.org/10.5219/140

Keywords:

lasalocid, residue, tissues of broiler chickens, screening, Total antibiotics

Abstract

The aim of the present study was to evaluate the microbial growth inhibition test Total antibiotics for the screening of lasalocid residues in the tissues of broiler chickens after its oral administration in medicated feed. The residues were investigated throughout the 5-day withdrawal period /WP/) and also on day 6 representing the first day following the WP. All broiler chicken tissues were positive for lasalocid. The breast muscle was positive (the presence of residues at/above the detection limit /LOD/ of method) up to day 1 of the WP, the thigh muscle, gizzard, heart, skin and fat up to day 3 of the WP and the liver and kidneys up to day 4 of the WP. When evaluating the dubious results (the presence of residues just below the LOD of method), the breast muscle was suspect positive up to day 3 of the WP and the gizzard, skin and fat up to day 4 of the WP. No positive or dubious results were detected on day 5 of the WP. The LOD of Bacillus stearothermophilus var. calidolactis for maduramycin was 500 µg.l^-1.

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