A randomised controlled trial of innovative specialised meat product for patients with cardiovascular and metabolic disorders
DOI:
https://doi.org/10.5219/1298Keywords:
CVD, metabolic disorder, functional food, hyperlipidemia, cholesterolAbstract
Cardiovascular diseases remain one of the leading causes of death globally. A lot of dietary patterns for CVD prevention have been proposed, but special attention is paid to functional foods. Bioactive proteins and peptides from animal sources are also considered tools for the prevention of CVDs. Here, 40 overweight or obese adult men and women aged between
61 and 66 years, with a body-mass index between 28 and 61 kg.m-2, were enrolled into a randomised controlled trial of new meat products for specialised nutrition. Participants in the control group (n = 20) consumed a standard hyponatric low-calorie diet for 28-30 days (10 days inpatient and 18-20 days outpatient), and in the experimental group – a low-calorie diet and 100g developed meat product (ratio of the porcine aorta to hearts 1:3) per day. Total cholesterol, triglyceride, cholesterol low-density lipoprotein, and cholesterol high-density lipoprotein levels were measured in the serum; from this, the atherogenic index was calculated. The positive effect of developed meat products on the serum lipid profile of patients during the trial was mild but noticeable. A significant reduction in cholesterol levels was noticed in the experimental group, by 18.2% and 14.0% after 7 – 10 and 28 – 30 days, respectively, while the cholesterol level in the control group returned to its original level after 28 – 30 days of dieting. The difference between the control and experimental groups was not significant, while data in the percentiles were. Therefore, it is more preferable to use a developed product as a component in diet therapy for hyperlipidaemic humans for over 28 – 30 days. Pronounced effects of the product could be linked to the unique proteome and peptidome of heart and aorta tissues based on organ-specific gene expression and the presence of tissue-specific substances.
Downloads
Metrics
References
Ahhmed, A. M., Muguruma, M. 2010. A review of meat protein hydrolysates and hypertension. Meat Science, vol. 86, no. 1, p. 110-118. https://doi.org/10.1016/j.meatsci.2010.04.032 DOI: https://doi.org/10.1016/j.meatsci.2010.04.032
Alissa, E. M., Ferns, G. A. 2012. Functional foods and nutraceuticals in the primary prevention of cardiovascular diseases. Journal of Nutrition and Metabolism, vol. 2012, p. 1-17. https://doi.org/10.1155/2012/569486 DOI: https://doi.org/10.1155/2012/569486
Anand, S. S., Hawkes, C., de Souza, R. J., Mente, A., Dehghan, M., Nugent, R., Zulyniak, M. A., Weis, T., Bernstein, A. M., Krauss, R. M., Kromhout, D., Jenkins, D. J. A., Malik, V., Martinez-Gonzalez, M. A., Mozaffarian, D., Yusuf, S., Willett, W. C., Popkin, B. M. 2015. Food consumption and its impact on cardiovascular disease: Importance of Solutions Focused on the Globalised Food System: A Report From the Workshop Convened by the World Heart Federation. Journal of the American College of Cardiology, vol. 66, no. 14, p. 1590-1614. https://doi.org/10.1016/j.jacc.2015.07.050 DOI: https://doi.org/10.1016/j.jacc.2015.07.050
Arihara, K. 2006. Strategies for designing novel functional meat products. Meat Science, vol.74, no. 1, p. 219-229. https://doi.org/10.1016/j.meatsci.2006.04.028 DOI: https://doi.org/10.1016/j.meatsci.2006.04.028
Arrutia, F., Fernández, R., Menéndez, C., González, U. A., Riera, F. A. 2017. Utilisation of blood by-products: An in silico and experimental combined study for BSA usage. Scientific Reports, vol. 7, p. 1-9. https://doi.org/10.1038/s41598-017-17029-2 DOI: https://doi.org/10.1038/s41598-017-17029-2
Barbeira, A. N., Dickinson, S.P., Bonazzola, R., Zheng, J., Wheeler, H. E., Torres, J.M., Torstenson, E. S., Shah, K. P., Garcia, T., Edwards, T. L., Stahl, E. A., Huckins, L. M., Nicolae, D. L., Cox, N. J., Im, H. K. 2018. Exploring the phenotypic consequences of tissue specific gene expression variation inferred from GWAS summary statistics. Nature Communications, vol. 9, no. 1, p. 1-20. https://doi.org/10.1038/s41467-018-03621-1 DOI: https://doi.org/10.1038/s41467-018-03621-1
Bauchart, C., Rémond, D., Chambon, C., Patureau Mirand, P., Savary-Auzeloux, I., Reynès, C., Morzel, M. 2006. Small peptides (<5 kDa) found in ready-to-eat beef meat. Meat Science, vol. 74, no. 4, p. 658-666. https://doi.org/10.1016/j.meatsci.2006.05.016 DOI: https://doi.org/10.1016/j.meatsci.2006.05.016
Bhat, Z. F., Kumar, S., Bhat, H. F. 2015. Bioactive peptides of animal origin: a review. Journal of Food Science and Technology, vol. 52, no. 9, p. 5377-5392. https://doi.org/10.1007/s13197-015-1731-5 DOI: https://doi.org/10.1007/s13197-015-1731-5
Breschi, A., Djebali, S., Gillis, J., Pervouchine, D. D., Dobin, A., Davis, C. A., Gingeras, T. R., Guigó, R. 2016. Gene-specific patterns of expression variation across organs and species. Genome Biology, vol. 17, no. 1, p. 1-13. https://doi.org/10.1186/s13059-016-1008-y DOI: https://doi.org/10.1186/s13059-016-1008-y
Cencic, A., Chingwaru, W. 2010. The role of functional foods, nutraceuticals, and food supplements in intestinal health. Nutrients, vol. 2, no. 6, p. 611-625. https://doi.org/10.3390/nu2060611 DOI: https://doi.org/10.3390/nu2060611
Chernukha, I. M., Fedulova, L. V., Kotenkova, E. A., Shishkin, S. S., Kovalyov, L. I., Mashentseva, N. G., Klabukova, D. L. 2016 Influence of heat treatment on tissue specific proteins in the Sus scrofa cardiac muscle and aorta. Russian Journal of Biopharmaceuticals, vol. 8, no. 6, p. 38-44.
Chernukha, I., Fedulova, L., Kotenkova, E., Akhremko, A. 2018. Hypolipidaemic action of the meat product: in vivo study. Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 566-569. https://doi.org/10.5219/959 DOI: https://doi.org/10.5219/959
Cicero, A. F. G., Fogacci, F., Colletti, A. 2017. Potential role of bioactive peptides in prevention and treatment of chronic diseases: a narrative review. British Journal of Pharmacology, vol. 174, no. 11, p. 1378-1394. https://doi.org/10.1111/bph.13608 DOI: https://doi.org/10.1111/bph.13608
Eilat-Adar, S., Sinai, T., Yosefy, C., Henkin, Y. 2013. Nutritional recommendations for cardiovascular disease prevention. Nutrients, vol. 5, no. 9, p. 3646-3683. https://doi.org/10.3390/nu5093646 DOI: https://doi.org/10.3390/nu5093646
Fagerberg, L., Hallström, B. M., Oksvold, P., Kampf, C., Djureinovic, D., Odeberg, J., et al. 2014. Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics. Molecular and Cellular Proteomics, vol. 13, no. 2, p. 397-406. https://doi.org/10.1074/mcp.M113.035600 DOI: https://doi.org/10.1074/mcp.M113.035600
Griffiths, K., Aggarwal, B. B., Singh, R. B., Buttar, H. S., Wilson, D., De Meester, F. 2016. Food antioxidants and their anti-inflammatory properties: A potential role in cardiovascular diseases and cancer prevention. Diseases (Basel, Switzerland), vol. 4, no. 3, p. 1-15. https://doi.org/10.3390/diseases4030028 DOI: https://doi.org/10.3390/diseases4030028
Guschanski, K., Warnefors, M., Kaessmann, H. 2017. The evolution of duplicate gene expression in mammalian organs. Genome Research, vol. 27, no. 9, p. 1461-1474. https://doi.org/10.1101/gr.215566.116 DOI: https://doi.org/10.1101/gr.215566.116
Han, T. S., Lean, M. E. 2016. A clinical perspective of obesity, metabolic syndrome and cardiovascular disease. JRSM Cardiovascular Disease, vol. 5, p. 1-13. https://doi.org/10.1177/2048004016633371 DOI: https://doi.org/10.1177/2048004016633371
Hongdong, S., Bo, L. 2017. Beneficial effects of collagen hydrolysate: A review on recent developments. Biomedical Journal of Scientific & Technical Research, vol. 1, no. 2, p.458-461. https://doi.org/10.26717/BJSTR.2017.01.000217 DOI: https://doi.org/10.26717/BJSTR.2017.01.000217
Hui, Y. H. 2012. Handbook of Meat and Meat Processing. 2nd ed. USA: Taylor & Francis Group, 1000 p. ISBN 9781439836835. DOI: https://doi.org/10.1201/b11479
Kotenkova, E., Chernukha, I. 2019. Influence of technological processing on lipid-lowering activity of substances containing in porcine hearts and aortas. Potravinarstvo Slovak Journal of Food Sciences, vol. 13, no. 1, p. 331-336. https://doi.org/10.5219/1119 DOI: https://doi.org/10.5219/1119
Koyama, Y., Kusubata, M. 2013. Effects of collagen peptide ingestion on blood lipids in rats fed a high-lipid and high-sucrose diet. Food Science and Technology Research, vol. 19, no. 6, p. 1149-1153. https://doi.org/10.3136/fstr.19.1149 DOI: https://doi.org/10.3136/fstr.19.1149
Lafarga, T., Hayes, M. 2014. Bioactive peptides from meat muscle and by-products: generation, functionality and application as functional ingredients. Meat Science, vol. 98, no. 2, p. 227-239. https://doi.org/10.1016/j.meatsci.2014.05.036 DOI: https://doi.org/10.1016/j.meatsci.2014.05.036
Liu, R., Xing, L., Fu, Q., Zhou, G. H., Zhang, W. G. 2016. A review of antioxidant peptides derived from meat muscle and by-products. Antioxidants (Basel, Switzerland), vol. 5, no. 3, p. 1-15. https://doi.org/10.3390/antiox5030032 DOI: https://doi.org/10.3390/antiox5030032
Lordan, R., Tsoupras, A., Mitra, B., Zabetakis, I. 2018. Dairy fats and cardiovascular disease: Do we really need to be concerned? Foods (Basel, Switzerland), vol. 7, no. 3, p. 1-34. https://doi.org/10.3390/foods7030029 DOI: https://doi.org/10.3390/foods7030029
Lyapina, L. A., Grigor’eva, M. E., Obergan, T. Y., Shubina, T. A., Andreeva, L. A., Myasoedov, N. F. 2015. Peptide regulation of metabolic processes under hypercholesterolaemia conditions of an organism. Biology Bulletin, vol. 42, no. 6, p. 546-555. https://doi.org/10.1134/S1062359015060060 DOI: https://doi.org/10.1134/S1062359015060060
Mozaffarian, D. 2016. Dietary and policy priorities for cardiovascular disease, diabetes, and obesity: A comprehensive review. Circulation, vol. 133, no. 2, p. 187–225. https://doi.org/10.1161/CIRCULATIONAHA.115.018585 DOI: https://doi.org/10.1161/CIRCULATIONAHA.115.018585
Mine, Y., Shahidi, F. 2006. Nutraceutical Proteins and Peptides in Health and Disease. USA: Taylor & Francis Group, CRC Press, 688 p. ISBN 9781420028836. DOI: https://doi.org/10.1201/9781420028836
Myasoedov, N. F., Lyapina, L. A., Grigorjeva, M. E., Obergan, T. Y., Shubina, T. A., Andreeva, L. A. 2016. Mechanisms for glyproline protection in hypercholesterolemia. Pathophysiology, vol. 23, no. 1, p. 27-33. https://doi.org/10.1016/j.pathophys.2015.11.001 DOI: https://doi.org/10.1016/j.pathophys.2015.11.001
Nakade, K. A., Kaneko, H., Oka, T., Ahhmed, A. M., Muguruma, M., Numata, M., Nagaoka, S. 2009. Сattle-heart hydrolysates ameliorates hypercholesterolaemia accompanied by suppression of the cholesterol absorption and rats in Caco-2 cells. Bioscience Biotechnology Biochemistry, vol. 73, no. 3, p. 607-612. https://doi.org/10.1271/bbb.80687 DOI: https://doi.org/10.1271/bbb.80687
Ryan, J. T., Ross, R. P., Bolton, D., Fitzgerald, G. F., Stanton, C. 2011. Bioactive peptides from muscle sources: meat and fish. Nutrients, vol. 3, no. 9, p. 765-791. https://doi.org/10.3390/nu3090765 DOI: https://doi.org/10.3390/nu3090765
Saklayen, M. G. 2018. The global epidemic of the metabolic syndrome. Current Hypertension Reports, vol. 20, no. 2, article ID 12, p. 1-8. doi:10.1007/s11906-018-0812-z DOI: https://doi.org/10.1007/s11906-018-0812-z
Sánchez, A., Vázquez, A. 2017. Bioactive peptides: A review. Food Quality and Safety, vol. 1, no. 1, p. 29-46. https://doi.org/10.1093/fqsafe/fyx006 DOI: https://doi.org/10.1093/fqs/fyx006
Shabalina, A. A., Liapina, L. A., Rochev, D. L., Kostyreva, M. V., Tanashian, M. M., Suslina, Z. A. 2015. In vitro lipid-lowering and fibrinolytic effects of regulatory leucine-containing glyprolines in human blood. Biology Bulletin, vol. 42, no. 1, p. 74-77. https://doi.org/10.1134/S1062359015010112 DOI: https://doi.org/10.1134/S1062359015010112
Sonawane, A. R., Platig, J., Fagny, M., Chen, C. Y., Paulson, J. N., Lopes-Ramos, C. M., DeMeo, D. L., Quackenbush, J., Glass, K., Kuijjer, M. L. 2017. Understanding tissue-specific gene regulation. Cell Reports, vol. 21, no. 4, p. 1077-1088. https://doi.org/10.1016/j.celrep.2017.10.001 DOI: https://doi.org/10.1016/j.celrep.2017.10.001
Toldrá, F., Aristoy, M. C., Mora, L., Reig, M. 2012. Innovations in value-addition of edible meat by-products. Meat Science, vol. 92, no. 3, p. 290-296. https://doi.org/10.1016/j.meatsci.2012.04.004 DOI: https://doi.org/10.1016/j.meatsci.2012.04.004
Tometsuka, C., Koyama, Y., Ishijima, T., Toyoda, T., Teranishi, M., Takehana, K., Abe, K., Nakai, Y. 2017. Collagen peptide ingestion alters lipid metabolism-related gene expression and the unfolded protein response in mouse liver. British Journal of Nutrition, vol. 117, no. 1, p. 1-11. https://doi.org/10.1017/S0007114516004384 DOI: https://doi.org/10.1017/S0007114516004384
Tomosugi, N., Yamamoto, S., Takeuchi, M., Yonekura, H., Ishigaki, Y., Numata, N., Katsuda, S., Sakai, Y. 2017. Effect of collagen tripeptide on atherosclerosis in healthy humans. Journal of Atherosclerosis and Thrombosis, vol. 24, no. 5, p. 530–538. https://doi.org/10.5551/jat.36293 DOI: https://doi.org/10.5551/jat.36293
Udenigwe, C. C., Howard, A. 2013. Meat proteome as source of functional biopeptides. Food Research International, vol. 54, no. 1, p. 1021-1032. https://doi.org/10.1016/j.foodres.2012.10.002 DOI: https://doi.org/10.1016/j.foodres.2012.10.002
Wahart, A., Hocine, T., Albrecht, C., Henry, A., Sarazin, T., Martiny, L., El Btaouri, H., Maurice, P., Bennasroune, A., Romier-Crouzet, B., Blaise, S., Duca, L. 2019. Role of elastin peptides and elastin receptor complex in metabolic and cardiovascular diseases. The FEBS Journal, vol. 286, no. 15, p. 2980-2993. https://doi.org/10.1111/febs.14836 DOI: https://doi.org/10.1111/febs.14836
Yazaki, M., Ito, Y., Yamada, M., Goulas, S., Teramoto, S., Nakaya, M. A., Ohno, S., Yamaguchi, K. 2017. Oral ingestion of collagen hydrolysate leads to the transportation of highly concentrated Gly-Pro-Hyp and its hydrolysed form of Pro-Hyp into the bloodstream and skin. Journal of Agricultural and Food Chemistry, vol. 65, no. 11, p. 2315-2322. https://doi.org/10.1021/acs.jafc.6b05679 DOI: https://doi.org/10.1021/acs.jafc.6b05679
Published
How to Cite
Issue
Section
License
This license permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
