Diversity of microorganisms in the traditional Slovak cheese
DOI:
https://doi.org/10.5219/1061Keywords:
diversity, microbiota, smoked and non-smoked cheese, mass spectrometryAbstract
The aim of the present study was to describe the microbial groups of the traditional Slovak cheese Parenica during rippening. The microbial group included the total bacterial count, coliform bacteria, enterococci, lactic acid bacteria, and microscopic filamentous fungi, which may affect the organoleptic characteristics of this product. A total of 42 cheese samples were collected from four different farms during three months. The total bacterial counts were cultivated on Plate count agar at 30 °C, lactic acid bacteria (LAB) on MRS, APT and MSE at 37 °C, coliform bacteria on VRBL at 37 °C. Gram-positive and Gram-negative isolates were identified by MALDI-TOF MS profiling. Bacillus sp. and Enterococcus faecium were the most frequently identified species of bacteria. Candida kefyr was the most distributed yeast according to microbiological methods. Lactic acid bacteria group was represented by Lactobacillus helveticus, L. jensenii, L. alimentarius, L. crispatus, L. curvatus, L. fermentum, L. suebicus, L. delbrueckii ssp. lactis, L. paracasei ssp. paracasei, Lactococcus lactis ssp. lactis, Leuconostoc lactis and Le. mesenteroides ssp. mesenteroides . This report describing the indigenous microbiota of the traditional raw milk cheeses from Slovakia. Our results provide useful information on occurrence of valuable microbial strain for the industrialization of producing of the traditional dairy products in Slovakia.
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Alessandria, V., Dolci, P., Rantsiou, K., Pattono, D., Dalmasso, A., Civera, T., Cocolin, L. 2010. Microbiota of the Planalto de Bolona: an artisanal cheese produced in uncommon environmental conditions in the Cape Verde Islands. World Journal of Microbiology and Biotechnology, vol. 26, no. 12, p. 2211-2221. https://doi.org/10.1007/s11274-010-0406-7 DOI: https://doi.org/10.1007/s11274-010-0406-7
Amato, L., Ritschard, J. S., Kurtz, O. Arias-Roth, E., Lacroix, C., Schuppler, M., Meile, L. 2012. Microbial composition of defect smear—a problem evolving during foil-prepacked storage of red-smear cheeses. International Dairy Journal, vol. 27, no. 1-2, p. 77-85. https://doi.org/10.1016/j.idairyj.2012.07.012 DOI: https://doi.org/10.1016/j.idairyj.2012.07.012
Bleicher, A., Obermajer, T., Matijasic, B. B., Siegfried Scherer, S., Neuhaus, K. 2010. High biodiversity and potent anti-listerial action of complex red smear cheese microbial ripening consortia. Annals of Microbiology, vol. 60, no. 3, p. 531-539. https://doi.org/10.1007/s13213-010-0083-7 DOI: https://doi.org/10.1007/s13213-010-0083-7
Buňková, L., Buňka, F. 2017. Microflora of processed cheese and the factors affecting it. Critical Reviews in Food sciences and Nutrition, vol. 57, no. 11, p. 2392-2403. https://doi.org/10.1080/10408398.2015.1060939 DOI: https://doi.org/10.1080/10408398.2015.1060939
Button, J. E., Dutton, R. J. 2012. Cheese microbes. Current Biology, vol. 22, no. 15, p. 587-589. https://doi.org/10.1016/j.cub.2012.06.014 DOI: https://doi.org/10.1016/j.cub.2012.06.014
Callon, C., Delbes, C., Duthoit, F., Montel, C. M. 2006. Application of SSCP–PCR fingerprinting to profile the yeast community in raw milk Salers cheese. Systematic and Applied Microbiology, vol. 29, no. 2, p. 172-180. https://doi.org/10.1016/j.syapm.2005.07.008 DOI: https://doi.org/10.1016/j.syapm.2005.07.008
Cogan, T. M., Goerges, S., Gelsomino, R., Donnelly, C. W. 2014. Biodiversity of the surface microbial consortia from Limburger, Reblochon, Livarot, Tilsit, and Gubbeen cheeses. Microbiology Spectrum, vol. 2, no.1. https://doi.org/10.1128/microbiolspec.CM-0010-2012 DOI: https://doi.org/10.1128/microbiolspec.CM-0010-2012
Colombo, F., Borgo, F., Fortina M. G. 2009. Genotypic characterization of non starter lactic acid bacteria involved in the ripening of artisanal Bitto PDO cheese. Journal of Basic Microbiology, vol. 49, no. 6, p. 521-530. https://doi.org/10.1002/jobm.200800381 DOI: https://doi.org/10.1002/jobm.200800381
De Freitas, I., Pinon, N., Maubois, J. L., Lortal, S., Thierry A. 2009. The addition of a cocktail of yeast species to Cantalet cheese changes bacterial survival and enhances aroma compound formation. International Journal of Food Microbiology, vol. 129, no. 6, 37-42. https://doi.org/10.1016/j.ijfoodmicro.2008.10.026 DOI: https://doi.org/10.1016/j.ijfoodmicro.2008.10.026
Delavenne, E., Mounier, J. Déniel, F. Barbier, G., Le Blay, G. 2012. Biodiversity of antifungal lactic acid bacteria isolated from raw milk samples from cow, ewe and goat over one-year period. International Journal of Food Microbiology, vol. 155, no. 3, p. 185-190. https://doi.org/10.1016/j.ijfoodmicro.2012.02.003 DOI: https://doi.org/10.1016/j.ijfoodmicro.2012.02.003
Feurer, C., Irlinger, F., Spinnler, H., Spinnler, E., Glaser, P., Vallaeys, T. 2004. Assessment of the surface microbial diversity in a farm house-produced vs a pasteurized industrially produced soft red-smear cheese using both cultivation and rDNA-based methods. Journal of Applied Microbiology, vol. 97, no. 3, p. 546-556. https://doi.org/10.1111/j.1365-2672.2004.02333.x DOI: https://doi.org/10.1111/j.1365-2672.2004.02333.x
Florez, A. B., Mayo, B. 2006. Microbial diversity and succession during the manufacture and ripening of traditional, Spanish, blue-veined Cabrales cheese, as determined by PCR-DGGE. International Journal of Food Microbiology, vol. 110, no. 2, p. 165-171. https://doi.org/10.1016/j.ijfoodmicro.2006.04.016 DOI: https://doi.org/10.1016/j.ijfoodmicro.2006.04.016
Franke, G., Cwiková, O. 2019. Biogenic amines in smear ripened cheeses. Potravinarstvo Slovak Journal of Food Sciences, vol. 13, no. 1, p. 378-384. https://doi.org/10.5219/1105 DOI: https://doi.org/10.5219/1105
Gkatzionis, K., Yunita, D., Linforth, R. S. T., Dickinson, M., Dodd, C. E. R. 2014. Diversity and activities of yeasts from different parts of a Stilton cheese. International Journal of Food Microbiology, vol. 177, p. 109-116. https://doi.org/10.1016/j.ijfoodmicro.2014.02.016 DOI: https://doi.org/10.1016/j.ijfoodmicro.2014.02.016
Goerges, S., Mounier, J., Rea, M., Gelsomino, C., Heise, V., Beduhn, R., Cogan, T. M., Vancanneyt, M., Scherer, S. 2008. Commercial ripening starter microorganisms inoculated into cheese milk do not successfully establish themselves in the resident microbial ripening consortia of a South German red smear cheese. Applied Environmental Microbiology, vol. 74, no. 7, p. 2210-2217. https://doi.org/10.1128/AEM.01663-07 DOI: https://doi.org/10.1128/AEM.01663-07
Gori, K., Ryssel, M., Arneborg, N., Jespersen, L. 2013. Isolation and identification of the microbiota of Danish farmhouse and industrially produced surface-ripened cheeses Microbial Ecology, vol 65, no. 3, p. 602-615. https://doi.org/10.1007/s00248-012-0138-3 DOI: https://doi.org/10.1007/s00248-012-0138-3
Chaves-López, C., Serio, A., Martuscelli, M., Paparella, A., Osorio-Cadavid, E., Suzzi, G. 2011. Microbiological characteristics of kumis, a traditional fermented colombian milk, with particular emphasis on enterococci population,” Food Microbiology, vol. 28, no. 5, p. 1041-1047. https://doi.org/10.1016/j.fm.2011.02.006 DOI: https://doi.org/10.1016/j.fm.2011.02.006
Irlinger, F., Morvan, A., El Solh, A. N., Bergere, J. L. 1997. Taxonomic characterisation of coagulase-negative staphylococci in ripening flora from traditional French cheeses. Systematic and Applied Microbiology, vol. 20, no. 2, p. 319-328. https://doi.org/10.1016/S0723-2020(97)80079-3 DOI: https://doi.org/10.1016/S0723-2020(97)80079-3
Larpin, S., Mondoloni, C., Goerges, S., Vernoux, J. P., Guguen, M., Desmasures, N. 2006. Geotrichum candidum dominates in yeast population dynamics in Livarot, a French red-smear cheese. FEMS Yeast Research, vol. 6, no. 8, p. 1243-1253. https://doi.org/10.1111/j.1567-1364.2006.00127.x DOI: https://doi.org/10.1111/j.1567-1364.2006.00127.x
Larpin-Laborde, S., Imran, M., Bonaïti, C., Bora, N., Gelsomino, R., Goerges, S., Irlinger, F., Goodfellow, M., Ward, A. C., Vancanneyt, M., Swings, J., Scherer, S., Guéguen, S., Desmasures, N. 2011. Surface microbial consortia from Livarot, a French smear ripened cheese. Canadian Journal of Microbiology, vol. 57, no. 8, p. 651-660. https://doi.org/10.1139/w11-050 DOI: https://doi.org/10.1139/w11-050
Lavoie, K., Touchette, M., St-Gelais, D., Labrie, S. 2012. Characterization of the fungal microflora in raw milk and specialty cheeses of the province of Quebec. Dairy Science and Technology, vol. 92, no. 5, p. 455-468. https://doi.org/10.1007/s13594-011-0051-4 DOI: https://doi.org/10.1007/s13594-011-0051-4
Lopandic, K., Zelger, S., Banszky, L. K., Eliskases-Lechner, F., Prillinger, H. 2006. Identification of yeasts associated with milk products using traditional and molecular techniques. Food Microbiology, vol. 23, no. 4, p. 341-350. https://doi.org/10.1016/j.fm.2005.05.001 DOI: https://doi.org/10.1016/j.fm.2005.05.001
Maoz, A., Mayr, R., Scherer, S. 2003. Temporal stability and biodiversity of two complex antilisterial cheese-ripening microbial consortia. Applied and Environmental Microbiology, vol. 69, no. 7, p. 4012-4018. https://doi.org/10.1128/AEM.69.7.4012-4018.2003 DOI: https://doi.org/10.1128/AEM.69.7.4012-4018.2003
Maragkoudakis, P. A., Miaris, C., Rojez, P., Manalis, N. Magkanari, F., Kalantzopoulos, G., Tsakalidou, E. 2006. Production of traditional Greek yoghurt using Lactobacillus strains with probiotic potential as starter adjuncts. International Dairy Journal, vol. 16, no. 1, p. 52-60. https://doi.org/10.1016/j.idairyj.2004.12.013 DOI: https://doi.org/10.1016/j.idairyj.2004.12.013
Mennane, Z., Khedid, K., Zinedine, A., Lagzouli, M., Ouhssine, M., Elyachioui, M. 2007. Microbial characteristics of klila and jben traditional Moroccan cheese from waw cow’s milk. World Journal of Dairy and Food Sciences, vol. 2, no. 1, p. 23-27.
Mirzaei, H. 2011. Microbiological changes in Lighvan cheese throughout its manufacture and ripening. African Journal of Microbiology Research, vol. 5, no. 13, 1609-1614. https://doi.org/10.5897/AJMR11.111 DOI: https://doi.org/10.5897/AJMR11.111
Montel, M. C., Buchin, S., Mallet, A., Delbes-Paus, C., Vuitton, D. A., Desmasures, N., Berthier, F. 2014. Traditional cheeses: rich and diverse microbiota with associated benefits. International Journal of Food Microbiology, vol. 177, p. 136-154. https://doi.org/10.1016/j.ijfoodmicro.2014.02.019 DOI: https://doi.org/10.1016/j.ijfoodmicro.2014.02.019
Mounier, J. Monnet, C., Jacques, N., Antoinette, A., Irlinger, F. 2009. Assessment of the microbial diversity at the surface of Livarot cheese using culture-dependent and independent approaches. International Journal of Food Microbiology, vol. 133, no. 1-2, p. 31-37. https://doi.org/10.1016/j.ijfoodmicro.2009.04.020 DOI: https://doi.org/10.1016/j.ijfoodmicro.2009.04.020
Mounier, J., Gelsomino, R., Goerges, S., Vancanneyt, M., Vandemeulebroecke, K., Hoste, B., Scherer, S., Swings, J., Fitzgerald, J. F., Cogan, T. M. 2005. Surface microflora of four smear-ripened cheeses. Applied and Environmental Microbiology, vol. 71, p. 6489-6500. https://doi.org/10.1128/AEM.71.11.6489-6500.2005 DOI: https://doi.org/10.1128/AEM.71.11.6489-6500.2005
Olson, N. F. 1990. The impact of lactic acid bacteria on fheese Flavor. FEMS Microbiology Reviews, vol. 87, no. 1-2, p.131-148. https://doi.org/10.1016/0378-1097(90)90702-R DOI: https://doi.org/10.1111/j.1574-6968.1990.tb04884.x
Ouadghiri, M., Vancanneyt, M., Vandamme, P., Naser, S., Gevers, D., Lefebvre, K., Swings, J., Amar, M. 2008. Identification of lactic acid bacteria in Moroccan raw milk and traditionally fermented skimmed milk ‘Lben’. Journal of Applied Microbiology, vol. 106, no. 2, p. 486-495. https://doi.org/10.1111/j.1365-2672.2008.04016.x DOI: https://doi.org/10.1111/j.1365-2672.2008.04016.x
Oyetunji, O. A., Adebisi, K. A. 2018. Assessment of the functional quality and safety of yoghurts produced with starter cultures obtained from selected commercially sold yoghurts. Potravinarstvo Slovak Journal of Food Sciences, vol. 12, no. 1, p. 587-599. https://doi.org/10.5219/952 DOI: https://doi.org/10.5219/952
Panelli, S., Buffoni, J. N., Bonacina, C., Feligini, B. 2012. Identification of moulds from the Taleggio cheese environment by the use of DNA barcodes. Food Control, vol. 28, no. 2, p. 385-391. https://doi.org/10.1016/j.foodcont.2012.05.022 DOI: https://doi.org/10.1016/j.foodcont.2012.05.022
Rea, M. C., Goerges, S., Gelsomino, R., Brennan, M. Mounier, J. Vancanneyt, M. Scherer, S. Swings, J., Cogan, T.M. 2007. Stability of the biodiversity of the surface consortia of Gubbeen, a red-smear cheese. Journal of Dairy Sciences, vol. 90, no. 5, p. 2200-2210. https://doi.org/10.3168/jds.2006-377 DOI: https://doi.org/10.3168/jds.2006-377
Roth, E., Schwenninger, S. M., Hasler, M., Eugster-Meier, E., Lacroix, C. 2010. Population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis. BMC Microbiology, vol. 10, no. 1, p. 74-87. https://doi.org/10.1186/1471-2180-10-74 DOI: https://doi.org/10.1186/1471-2180-10-74
Sun, Z., Liu, W., Gao, W., Yang, M., Zhang, J., Wu, L., Wang, J., Menghe, B., Sun T., Zhang, H. 2010. Identification and characterization of the dominant lactic acid bacteria from Kurut: the naturally fermented yak milk in Qinghai, China. Journal of General and Appllied Microbiology, vol. 56, no. 1, p. 1-10. https://doi.org/10.2323/jgam.56.1 DOI: https://doi.org/10.2323/jgam.56.1
Štefániková, J., Nagyová, V., Hynšt, M., Vietoris, V., Martišová, P., Nagyová, Ľ. 2019. Application of electronic nose for determination of Slovak cheese authentication based on aroma profile. Potravinarstvo Slovak Journal of Food Sciences, vol. 13, no. 1, p. 262-267. https://doi.org/10.5219/1076 DOI: https://doi.org/10.5219/1076
Urbach, G. 1995. Contribution of lactic acid bacteria to flavour compound formation in dairy products. International Dairy Journal, vol. 5, no. 8, p. 877-903. https://doi.org/10.1016/0958-6946(95)00037-2 DOI: https://doi.org/10.1016/0958-6946(95)00037-2
Valdés-Stauber, N., Scherer, S., Seiler, H. 1997. Identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses. International Journal of Food Microbiology, vol. 34, no. 2, p. 115-129. https://doi.org/10.1016/S0168-1605(96)01171-3 DOI: https://doi.org/10.1016/S0168-1605(96)01171-3
Viljoen, B. C., Khoury, A. R., Hattingh, A. 2003. Seasonal diversity of yeasts associated with white-surface mould-ripened cheeses. Food Research International, vol. 36, no. 2, p. 275-283. https://doi.org/10.1016/S0963-9969(02)00169-2 DOI: https://doi.org/10.1016/S0963-9969(02)00169-2
Widyastuti, Y., Rohmatussolihat, Febrisiantosa, A. 2014. The role of lactic acid bacteria in milk fermentation. Food and Nutrition Sciences, vol. 5, no. 4, p. 248-278.
Wouters, J. T. M., Ayad, E. H. E., Hugenholtz, J., Smit, G. 2002. Microbes from raw milk for fermented dairy products. International Dairy Journal, vol. 12, no. 2-3, p. 91-109. https://doi.org/10.1016/S0958-6946(01)00151-0 DOI: https://doi.org/10.1016/S0958-6946(01)00151-0
Wyder, M. T. 2003. Yeasts in dairy products. Woodhead Publishing Series in Food Science, Technology and Nutrition, p. 209-237. https://doi.org/10.1533/97 81845698485.209 DOI: https://doi.org/10.1533/9781845698485.209
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