TY - JOUR AU - Lavová, Monika AU - Bezeková, Jana AU - Čanigová, Margita AU - Kročko, Miroslav AU - Domig, Konrad PY - 2014/05/02 Y2 - 2024/03/28 TI - Species identification of enterococci by biochemical test and molecular-genetic methods JF - Potravinarstvo Slovak Journal of Food Sciences JA - Potr. S. J. F. Sci. VL - 8 IS - 1 SE - Articles DO - 10.5219/364 UR - https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/364 SP - 124-129 AB - <p>The aim of this study was comparison different methods of species identification of enterococci. One hundred and fifty three suspected colonies were isolated from milk and dairy products (cheeses from cow´s, ewe´s and goat´s milk). On the bases of their growth on BEA agar, microscopic characteristic, results of Gram staining, catalase test and PYRAtest was thirty four isolates assigned to the genus <em>Enterococcus</em>. These isolates were identified by commercial biochemical test EN-COCCUS. 52.9% of them were included in species <em>E. faecalis</em>, 29.4% in <em>E. faecium</em>, 14.7% in <em>E. durans</em> and 2.9% in E. group III. This group includes 3 species: <em>E. durans, E. hirae, E. faecalis asaccharolytic var.</em> Then 16S rRNA sequencing nucleotide of all isolates was realized. Results of sequencing were compared with NCBI database. Only 14.7% of isolates were in 100% accordance. One from them was species <em>E. durans</em> and others were designated as <em>E. faecium</em>. For 20.6% of detected isolates was in accordance with more reference strains. Other isolates were identical with reference strain on 99%. For verification of all results species-specific PCR was used and 52.9% isolates were identified as species <em>E. faecalis</em>, 32.4% as <em>E. faecium</em> and 14.7% as <em>E. durans</em>. Strains belonging to the species <em>E. faecalis</em> were identified the most reliable by all used methods.</p> ER -