TY - JOUR AU - Pukáčová, Jana AU - Poľaková, Lucia AU - Dudriková, Eva PY - 2010/11/13 Y2 - 2024/03/28 TI - Sensitivy to antibiotics in strains of S. aureus isolated from cow´s milk JF - Potravinarstvo Slovak Journal of Food Sciences JA - Potr. S. J. F. Sci. VL - 4 IS - 4 SE - Articles DO - 10.5219/21 UR - https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/21 SP - 56-64 AB - <p>Strains of <em>S. aureus</em> were isolated from individual milk samples of 500 lacting cows from different areas in Slovakia (PH 1 - Košice region, 300 samples; PH 2 - Žilina region, 200 samples). The statistical significance between both dairy farms included in the experiment in the presence of genus <em>Staphylococcus</em> isolated on Baird-Parker agar from milk samples was p &lt; 0,0001<sup>(</sup><sup>***)</sup><sup> </sup>. Totally, 122 milk samples were positive for the presence of <em>Staphylococcus aureus</em> (83 Košice regions, 52 Žilina regions). All 122 isolates of <em>S. aureus</em> were sensitive for the methicilin as detected by interpretative criteria developed by NCCLS (2002). For 122 <em>S. aureus</em> isolates, we compared antibiotic susceptibility results determined by the standardized agar diffusion assay with the PCR assay for the detection of antibiotic resistance <em>mec</em>A gene. For all isolates, we found a correlation between the results of the PCR and those of classical resistance testing. The obtained results were confirmed by PCR analysis, according to which, any of our tested isolate of <em>S. aureus</em> from all 122 individual milk samples from both experimental dairy farms were not positive for the presence of <em>mec</em>A gene coding  the methicilin resistance.</p> ER -