@article{Zeleňáková_Židek_Čanigová_Paulov_Gallisová_2010, title={Evaluation of elisa method to detection of cow β-lactoglobulin in sheep milk and sheep milk products}, volume={4}, url={https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/78}, DOI={10.5219/78}, abstractNote={<p class="A8SUMMARY" style="margin: 0cm 0cm 3pt;">The aim of work was to optimalize the ELISA method to detect the adulteration of sheep milk and sheep milk products by cow milk in the laboratory. We have focused on laboratory testing of ELISA kit (β-Lactoglobulin ELISA Set, SEDIUM R&D) for detection of cow β-Lg in sheep milk order to obtain high-quality, reliable and economically advantageous method suitable for routine use in practice. The results shown that for the quality of adulteration determination  it is necessary to verify the sensitivity of applied kit by the samples dilution in accordance with the producer declared quantification range contained in the manual ELISA kit. The starting point for obtaining of relevant data was to create separate regression curves with high deter­mination coefficient, which allowed to quickly and easily detect the cow milk additions in sheep milk, cloddish sheep and Slovak sheep cheese.</p>}, number={4}, journal={Potravinarstvo Slovak Journal of Food Sciences}, author={Zeleňáková, Lucia and Židek, Radoslav and Čanigová, Margita and Paulov, Juraj and Gallisová, Tatiana}, year={2010}, month={Nov.}, pages={80–84} }