@article{Bajzí­k_Židek_Golian_Belej_Čapla_Maršálková_Revák_2011, title={Optimalisation of species identification of common carp (Cyprinus carpio) using SYBR® green I real-time PCRmethod}, volume={5}, url={https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/154}, DOI={10.5219/154}, abstractNote={<p>European Union Member States, together with a number of countries around the world, places great emphasis on ensuring the protection of consumers as a potential food allergic from food allergies. to inform consumers that their product may contain any of the risk of allergenic ingredients. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR<sup>®</sup> Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (<em>Cyprinus carpio</em>), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. Results of using the optimized SYBR ® Green I Real-Time PCR method for species identification common carp (<em>Cyprinus carpio</em>) indicate to its suitability.</p>}, number={3}, journal={Potravinarstvo Slovak Journal of Food Sciences}, author={Bajzí­k, Pavol and Židek, Radoslav and Golian, Jozef and Belej, Ľubomí­r and Čapla, Jozef and Maršálková, Lenka and Revák, Ondrej}, year={2011}, month={Jul.}, pages={1–5} }