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<article xml:lang="en" article-type="research-article" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">
    <front>
        <journal-meta>
            <journal-id journal-id-type="publisher-id">PSJFS</journal-id>
            <journal-title-group>
                <journal-title>Potravinarstvo Slovak Journal of Food Sciences</journal-title>
                <abbrev-journal-title abbrev-type="pubmed">Potr. S. J. F. Sci.</abbrev-journal-title>
            </journal-title-group>
            <issn pub-type="ppub">1338-0230</issn>
            <issn pub-type="epub">1337-0960</issn>
            <publisher>
                <publisher-name>Association HACCP Consulting</publisher-name>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="publisher-id">PSJFS-13-1-1051</article-id>
            <article-id pub-id-type="doi">10.5219/1263</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>ARTICLE</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>AUTHENTICATION AND PREFERENCE MAPPING OF HAM</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-2321-6627</contrib-id>
                    <name>
                        <surname>Bene&#x0161;ov&#x00E1;</surname>
                        <given-names>Lucia</given-names>
                    </name>
                    <xref ref-type="corresp" rid="cor1">&#x002A;</xref>
                </contrib>
                <contrib contrib-type="author">
                    <contrib-id contrib-id-type="orcid">http://orcid.org/0000-0001-6284-2578</contrib-id>
                    <name>
                        <surname>Golian</surname>
                        <given-names>Jozef</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff2" />
                </contrib>
                <contrib contrib-type="author">
                    <contrib-id contrib-id-type="orcid">http://orcid.org/0000-0001-7810-6858</contrib-id>
                    <name>
                        <surname>Marti&#x0161;ov&#x00E1;</surname>
                        <given-names>Patr&#x00ED;cia</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff3" />
                </contrib>
                <contrib contrib-type="author">
                    <contrib-id contrib-id-type="orcid">http://orcid.org/0000-0003-4941-3394</contrib-id>
                    <name>
                        <surname>Semjon</surname>
                        <given-names>Boris</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff4" />
                </contrib>
                <contrib contrib-type="author">
                    <contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-4425-4374</contrib-id>
                    <name>
                        <surname>Zaj&#x00E1;c</surname>
                        <given-names>Peter</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff5" />
                </contrib>
                <contrib contrib-type="author">
                    <contrib-id contrib-id-type="orcid">http://orcid.org/0000-0001-9475-6359</contrib-id>
                    <name>
                        <surname>&#x010C;apla</surname>
                        <given-names>Jozef</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff6" />
                </contrib>
                <contrib contrib-type="author">
                    <contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-8214-8169</contrib-id>
                    <name>
                        <surname>Vĺ&#x010D;ko</surname>
                        <given-names>Tom&#x00E1;&#x0161;</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff7" />
                </contrib>
                <aff id="aff2">
                    <institution>Jozef Golian, Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 37 641 4325, E-mail: jozef.golian@uniag.sk</institution>
                </aff>
                <aff id="aff3">
                    <institution>Patr&#x00ED;cia Marti&#x0161;ov&#x00E1;, Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Technology and Quality and Plant Products, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.:+421376414608, E-mail: xmartisovap@uniag.sk</institution>
                </aff>
                <aff id="aff4">
                    <institution>Boris Semjon, University of Veterinary Medicine and Pharmacy in Ko&#x0161;ice, Department of Food Hygiene and Technology, Komensk&#x00E9;ho 73, 041 81 Ko&#x0161;ice, Slovakia, Tel.: +421903919039, E-mail: boris.semjon@uvlf.sk</institution>
                </aff>
                <aff id="aff5">
                    <institution>Peter Zaj&#x00E1;c, Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 37 641 4371, E-mail: peter.zajac@uniag.sk</institution>
                </aff>
                <aff id="aff6">
                    <institution>Jozef &#x010C;apla, Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Hygiene and Food Safety, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: +421 37 641 4371, E-mail: jozef.capla@uniag.sk</institution>
                </aff>
                <aff id="aff7">
                    <institution>Tom&#x00E1;&#x0161; Vĺ&#x010D;ko, Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, E-mail: xvlckot@uniag.sk</institution>
                </aff>
            </contrib-group>
            <author-notes>
                <corresp id="cor1">
                    <label>&#x002A;</label>Corresponding author: Lucia Bene&#x0161;ov&#x00E1;, Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Food Hygiene and Safety, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia, Tel.: <phone>+421376414608</phone>, E-mail: <email xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="xbenesova@uniag.sk">xbenesova@uniag.sk</email></corresp>
            </author-notes>
            <pub-date pub-type="epub">
                <day>28</day>
                <month>12</month>
                <year>2019</year>
            </pub-date>
            <pub-date pub-type="ppub">
                <month>12</month>
                <year>2019</year>
            </pub-date>
            <volume>13</volume>
            <issue>1</issue>
            <fpage>1051</fpage>
            <lpage>1056</lpage>
            <history>
                <date date-type="received">
                    <day>15</day>
                    <month>9</month>
                    <year>2019</year>
                </date>
                <date date-type="accepted">
                    <day>12</day>
                    <month>12</month>
                    <year>2019</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>&#x00A9; Association HACCP Consulting. All rights reserved.</copyright-statement>
                <copyright-year>2019</copyright-year>
                <license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">
                    <license-p>This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<uri xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0</uri>) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <abstract>
                <p>Effective connection between the food industry and consumer demands are specific needs of consumers whitch were monitored in this study by using a preferential mapping method. Preference mapping is based on Principal Component Analysis (PCA), which is performed on preferences ratings given for each product and preferences of each consumer through an online questionnaire. Key features for the consumer choice were colour, odour, consistency, total flavour and overall appearance. We verified the composition and mapped the preferences of 10 hams purchased in Slovakia. In view of the persistence of identified cases of food counterfeiting and meat fraud, intensive monitoring and scrutiny is required through effective and accurate analytical methods, which are crucial for maintaining consumer confidence and ensuring compliance with local legislation and labeling. The reference approach for identifying animal species in food is the PCR method, which is however limited to several animal species, meat types. The use of microarray technology enables the identification of a wider range of animal species and greater user comfort, especially the speed of obtaining the results. It allows 24 animal species to be identified in one analysis in 8 samples at a time. Detection was performed using Chipron LCD Aarray Kit Meat 5.0. In all analyzed samples, components of animal origin were identified in accordance on the packaging of the products. The Meat 5.0 LCD chip, which was used for analysis, has detected the presence of other animal species.</p>
            </abstract>
            <kwd-group>
                <kwd>ham</kwd>
                <kwd>consumer preference</kwd>
                <kwd>sensory</kwd>
                <kwd>PCA</kwd>
                <kwd>DNA</kwd>
                <kwd>animal species</kwd>
            </kwd-group>
        </article-meta>
    </front>
    <body>
        <sec sec-type="intro">
            <title>INTRODUCTION</title>
            <p>Consumer perception of food quality is different and strongly dependent on personal preferences such as level of experience, cultural influences, demographic and physiological characteristics, product perception and quality expectations. It may be affected by several factors, e.g. brand origin, price, nutritional information and traditional technological processes (<xref ref-type="bibr" rid="r30">Supekov&#xE1;, 2008</xref>).</p>
            <p>In the overall quality of the meat, taste plays a major role, and therefore the presence of sensory defects and/or lack of typical taste significantly reduces its quality, causing financial losses for the dairy industry (<xref ref-type="bibr" rid="r9">Engel et al., 2001</xref>). The evaluation of sensory attributes makes it possible to define the taste profile and consumer preferences for dairy products with innovative properties. Meat sensory profile analysis allows to identify specific attributes that could be preferential properties and evaluate the impact of health information on consumer preferences, expectations and choices (<xref ref-type="bibr" rid="r29">Santillo and Albenzio, 2015</xref>). Large data sets are becoming increasingly common and often difficult to interpret. Principal Component Analysis (PCA) is a technique to reduce the size of such data sets, thereby increasing interpretability but at the same time information loss is minimized (<xref ref-type="bibr" rid="r17">Jolliffe and Cadima, 2016</xref>).</p>
            <p>Preferential mapping is a set of statistical methods aimed to detecting consumer preferences of the products being compared using sensory profiles. This method is used in the food industry to develop new products, especially according to consumer requirements (<xref ref-type="bibr" rid="r21">Meullenet et al., 2007</xref>). Preferential mapping is carried out by trained evaluators to better understand consumer acceptability of products. Product placement on the market determines their usability (<xref ref-type="bibr" rid="r19">MacFie, 2007</xref>). It is also a key management tool that is often used to optimize products by combining consumer and sensory data (<xref ref-type="bibr" rid="r12">Greenhoff and MacFie, 1994</xref>). Consumer segmentation strategies aim to identify sectors of the population of consumers with different criteria of preferences (<xref ref-type="bibr" rid="r7">Carbonell et al., 2007</xref>). Using a preferential map, it is possible to describe consumer preferences for a set of competing products from the sensory profiles of these products, based on a trained panel of evaluators. Preferential mapping refers to a group of multivariate statistical techniques that provide a comprehensive overview of both external and internal mapping. External preference mapping is based on multidimensional product display based on their sensory profile or a set of other external data, such as instrumental analysis using electronic tongue, nose and eye. This result is usually obtained through Principal Component Analysis (PCA). However, with this technique, it is possible to reduce each consumer&#x27;s hedonic assessment to a set of descriptive attributes (<xref ref-type="bibr" rid="r6">Cadena et al., 2012</xref>). External mapping approaches are limited by the fact that the sensory space (i.e., multidimensional representation) is obtained only from external data without the preference of attributes based on their importance to consumers (<xref ref-type="bibr" rid="r21">Meullenet et al., 2007</xref>). Internal preference mapping brings a multidimensional depiction of products and consumers. This representation is obtained by means of a PCA data matrix with products such as rows and consumers as columns. For that consumer, the data is based on from the hedonic score (<xref ref-type="bibr" rid="r12">Greenhoff, MacFie, 1994</xref>).</p>
            <p>Meat and meat products have a profound impact on human nutrition and hence on consumer health. Meat is a rich source of protein, containing all essential amino acids is a good source of iron, phosphorus, zinc, selenium, riboflavin, niacin, vitamin B6, vitamin B12, choline and others. Red meats such as beef, pork and mutton contain many essential nutrients essential for healthy growth and development in children. Red meat is one of the best sources of iron and zinc that is well absorbed in the body. Meat is also used as part of many foods and meat products (<xref ref-type="bibr" rid="r20">Mansoor et al., 2015</xref>). Given the high commercial value, meat has attracted the attention of counterfeiters for centuries (<xref ref-type="bibr" rid="r2">Barai et al., 1992</xref>). Identifying the origin of meat and meat products is an important issue for the prevention and detection of fraud that could have economic, ethical and health implications (<xref ref-type="bibr" rid="r3">Bertolini et al., 2015</xref>). Currently, there are several methods capable of recognizing chicken, pork and beef, which are among the most consumed meats in the world. Various analytical techniques have been proposed that identify these meats either alone or in mixtures (<xref ref-type="bibr" rid="r14">Hsieh, 2006;</xref> <xref ref-type="bibr" rid="r13">G&#xFC;nssen et al. 2006</xref>).</p>
            <p>Hygiene and proper labeling on the label of food products are very important aspects in particular for public health. Food safety covers all precautions for food supply and ensuring health and hygiene conditions for consumers (<xref ref-type="bibr" rid="r26">&#xD6;zpinar et al., 2013</xref>). Adulteration detection is a demanding industry in the food industry. Active development of additives, as well as novel foods that have undergone significant changes in the food matrix, increase the demands for accuracy and reliability of analytical methods based on the identification of sensory, anatomical, morphological and histological differences in the detection of counterfeiting (<xref ref-type="bibr" rid="r32">Yosef, 2014</xref>). Testing techniques are becoming faster, more accurate, more sensitive, more userfriendly, capable of detecting more than one species in one reaction (<xref ref-type="bibr" rid="r15">&#x130;lhak and Arslan, 2007</xref>). These tests are also available as commercial test kits, are suitable for routine analysis due to their ease of use, speed and relatively low cost, but limited use for highly processed foods (<xref ref-type="bibr" rid="r23">Montowska et al., 2014</xref>).</p>
            <sec>
                <title>Scientific hypothesis</title>
                <p>
                    <list list-type="simple">
                        <list-item>
                            <p>Hypothesis 1: To map the preferences of consumers of hams produced in Slovakia using the preferential mapping method.</p>
                        </list-item>
                        <list-item>
                            <p>Hypothesis 2: Verified the composition of 10 hams.</p>
                        </list-item>
                        <list-item>
                            <p>Hypothesis 3: Determine whether there is a link between consumer preferences and the composition of the product.</p>
                        </list-item>
                    </list>
                </p>
            </sec>
            <sec sec-type="materials|methods">
                <title>MATERIAL AND METHODOLOGY</title>
                <sec>
                    <title>Preference mapping</title>
                    <sec>
                        <title>Internal mapping</title>
                        <p>Samples were prepared from 10 hams produced in Slovakia. Samples were served on white ceramic plates, coded with three-digit random numbers and served at temperature of consumption 20 &#xB1;2 &#xB0;C. The evaluation was carried out in a standardized sensory laboratory (<xref ref-type="bibr" rid="r16">ISO 8589, 2007</xref>) built in the Slovak University of Agriculture in Nitra on Department of Food Safety and Hygiene. 13 assesors participated in the analyzes, who evaluated 10 ham samples. They evaluated the following sensory qualities: colour, odour, consistency, total flavour and overall appearance, which they could assign 1 – 9 points (1 – very bad and 9 – very good).</p>
                    </sec>
                    <sec>
                        <title>External mapping</title>
                        <p>External mapping (consumer survey) was conducted through an electronic questionnaire in which 140 respondents were addressed, from whom a statement on the products was requested. Their task was to organize the individual products according to their personal preferences (from 1 – best to 9 – worst).</p>
                    </sec>
                </sec>
                <sec>
                    <title>Species identification</title>
                    <p>Ten samples were purchased in various retail networks. The identified animal species were compared to the product composition of the manufacturer. In the first step of the analysis, we used Maxwell 16 DNA Purification kit (Promega, Wisconsin, USA) to achieve optimal DNA purity obtained from the purchased products. A PCR product was generated from the isolated DNA using the Aarray Kit Meat 5.0 (Chipron, Berlin, Germany), which was verified by gel electrophoresis followed by the protocol of the Chipron LCD manufacturer Aarray Kit Meat 5.0, allowing 24 species (cattle, sheep, equine, goat, camel, water buffalo, pork, kangaroo, hare, rabbit, reindeer, roe deer, red deer, fallow deer, springbok, canine, cat, chicken, turkey, goose, ostrich, mallard duck, muscovy duck and pheasant) to be identified. We used instrumentation, scanner and software (SlideReader V12) designed and recommended by the manufacturer for evaluation.</p>
                </sec>
            </sec>
            <sec>
                <title>Statistic analysis</title>
                <p>XLSTAT statistical software (2019.1.1, Addinsoft) was used to process data from both internal, external evaluation and authentication. PCA (Principal Component Analysis) was used for internal data from sensory analysis and AHC (Agglomerative Hierarchical Clustering) for external data (questionaire). Preferential map was created by combining these two outputs. ANOVA was used to determine if there was a statistically significant difference between the samples.</p>
            </sec>
        </sec>
        <sec sec-type="results|discussion">
            <title>RESULTS AND DISCUSSION</title>
            <p>Table <xref ref-type="table" rid="T1">1</xref> show summary evaluation of sensory analysis. From the PCA (Figure <xref ref-type="fig" rid="F1">1</xref>) we can observe that three groups of samples have been specified. Samples 2, 4, 8, 9, and 10 show obtained a higher rating from samples in colou, odour, consistency, total flavor and overall appearance.</p>
            <table-wrap id="T1" position="float">
                <label>Table 1</label>
                <caption>
                    <p>Summary evaluation of sensory analysis</p>
                </caption>
                <table frame="hsides" rules="none" width="100%">
                    <thead>
                        <tr>
                            <th rowspan="5">Samples</th>
                            <th colspan="10">Sensory qualities</th>
                        </tr>
                        <tr>
                            <th colspan="10">
                                <hr/>
                            </th>
                        </tr>
                        <tr>
                            <th colspan="2">Color</th>
                            <th colspan="2">Odour</th>
                            <th colspan="2">Consistency</th>
                            <th colspan="2">Total flavour</th>
                            <th colspan="2">Overall appearance</th>
                        </tr>
                        <tr>
                            <th colspan="10">
                                <hr/>
                            </th>
                        </tr>
                        <tr>
                            <th>Average</th>
                            <th>Variance</th>
                            <th>Average</th>
                            <th>Variance</th>
                            <th>Average</th>
                            <th>Variance</th>
                            <th>Average</th>
                            <th>Variance</th>
                            <th>Average</th>
                            <th>Variance</th>
                        </tr>
                        <tr>
                            <th colspan="11">
                                <hr/>
                            </th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr align="center">
                            <td>1</td>
                            <td>6.2</td>
                            <td>2.40</td>
                            <td>5.7</td>
                            <td>5.57</td>
                            <td>6.1</td>
                            <td>5.43</td>
                            <td>5.3</td>
                            <td>6.23</td>
                            <td>5.4</td>
                            <td>5.82</td>
                        </tr>
                        <tr align="center">
                            <td>2</td>
                            <td>6.8</td>
                            <td>2.18</td>
                            <td>7.1</td>
                            <td>0.77</td>
                            <td>6.7</td>
                            <td>2.68</td>
                            <td>6.4</td>
                            <td>2.71</td>
                            <td>6</td>
                            <td>4.89</td>
                        </tr>
                        <tr align="center">
                            <td>3</td>
                            <td>5.8</td>
                            <td>2.84</td>
                            <td>4.8</td>
                            <td>5.29</td>
                            <td>5.7</td>
                            <td>6.01</td>
                            <td>5.1</td>
                            <td>4.99</td>
                            <td>5.2</td>
                            <td>4.40</td>
                        </tr>
                        <tr align="center">
                            <td>4</td>
                            <td>7.1</td>
                            <td>1.88</td>
                            <td>6.8</td>
                            <td>5.29</td>
                            <td>6.9</td>
                            <td>2.99</td>
                            <td>7.5</td>
                            <td>2.06</td>
                            <td>7.1</td>
                            <td>3.21</td>
                        </tr>
                        <tr align="center">
                            <td>5</td>
                            <td>6.3</td>
                            <td>3.12</td>
                            <td>5.5</td>
                            <td>0.72</td>
                            <td>6.4</td>
                            <td>5.82</td>
                            <td>5.8</td>
                            <td>2.40</td>
                            <td>5.4</td>
                            <td>2.71</td>
                        </tr>
                        <tr align="center">
                            <td>6</td>
                            <td>6.1</td>
                            <td>4.32</td>
                            <td>5.3</td>
                            <td>2.68</td>
                            <td>5.7</td>
                            <td>5.79</td>
                            <td>4.8</td>
                            <td>3.73</td>
                            <td>5.5</td>
                            <td>3.61</td>
                        </tr>
                        <tr align="center">
                            <td>7</td>
                            <td>6.3</td>
                            <td>2.23</td>
                            <td>5.8</td>
                            <td>3.07</td>
                            <td>6.5</td>
                            <td>2.28</td>
                            <td>5.8</td>
                            <td>1.51</td>
                            <td>6</td>
                            <td>2.22</td>
                        </tr>
                        <tr align="center">
                            <td>8</td>
                            <td>6.7</td>
                            <td>2.68</td>
                            <td>6.5</td>
                            <td>1.61</td>
                            <td>6.8</td>
                            <td>3.73</td>
                            <td>6.7</td>
                            <td>1.79</td>
                            <td>6.6</td>
                            <td>2.49</td>
                        </tr>
                        <tr align="center">
                            <td>9</td>
                            <td>6.8</td>
                            <td>2.62</td>
                            <td>6.3</td>
                            <td>5.12</td>
                            <td>7.1</td>
                            <td>3.66</td>
                            <td>7</td>
                            <td>3.33</td>
                            <td>7.2</td>
                            <td>2.62</td>
                        </tr>
                        <tr align="center">
                            <td>10</td>
                            <td>7.3</td>
                            <td>1.57</td>
                            <td>7</td>
                            <td>3.33</td>
                            <td>7</td>
                            <td>1.33</td>
                            <td>6.6</td>
                            <td>4.27</td>
                            <td>6.5</td>
                            <td>4.94</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <fig id="F1" position="float">
                <label>Figure 1</label>
                <caption>
                    <p>Evaluation using PCA.</p>
                </caption>
                <graphic xlink:href="PSJFS-13-1-1051_F1.jpg"/>
            </fig>
            <p>The resulting processing of internal and external data is a map of preferences (Figure <xref ref-type="fig" rid="F2">2</xref>).</p>
            <fig id="F2" position="float">
                <label>Figure 2</label>
                <caption>
                    <p>Preference mapping of hams</p>
                </caption>
                <graphic xlink:href="PSJFS-13-1-1051_F2.jpg"/>
            </fig>
            <p>From the graphical representation of the results, we can conclude that sample 10 was placed in the highest consumer preference zone (80 – 100%) and samples 2, 4, 8 and 9 in the preference zone from 60 – 80% based on the characteristics of the surveyed products. Samples placed in the lowest consumer preference zone (0 – 40%) were 1, 3, 5, 6, 7 and recorded also lowest scores for overall appearance, odour, consistency, flavour and colour. The ANOVA test results show that there is a statistically significant difference between the samples (<italic>p</italic>-value = 0.033).</p>
            <p>Our results are consistent with studies done abroad. These techniques have recently been applied to dulce de leche (<xref ref-type="bibr" rid="r11">Gaze et al., 2015</xref>), ice cream (<xref ref-type="bibr" rid="r6">Cadena, et al., 2012</xref>), apples (<xref ref-type="bibr" rid="r4">Bonany et al., 2014</xref>), raspberries (<xref ref-type="bibr" rid="r31">Villamor, et al., 2013</xref>), tomatoes (<xref ref-type="bibr" rid="r25">Oltman, Yates and Drake, 2016</xref>) and have shown that they provide a very good understanding of the attributes that lead to popularity among consumers.</p>
            <p>DNA Microarray and Real Time PCR methods differentiate from each other in simultaneously detection of animal species in one reaction. The only common similarity between them is the step of DNA isolation. Microarray Analysis can enable us for detecting more than one species in one reaction only whereas Real Time PCR requires specially designed primers and probes needed to simultaneously amplify the specially selected regions of DNAs belonging to different species. This difference means longer time needed in the optimization step of primers and probes (<xref ref-type="bibr" rid="r24">Myers et al., 2010</xref>). DNA Microarray can deliver the results faster and more sensitive using amplified DNA by conventional PCR technique (<xref ref-type="bibr" rid="r1">Azuka et al., 2011</xref>). DNA Microarray makes possible the whole genome to be displayed on a chip and to express the interaction of thousands of genes with each other simultaneously (<xref ref-type="bibr" rid="r27">Pereira, Carneiro and Amorim, 2008;</xref> <xref ref-type="bibr" rid="r22">Miller and Tang 2009</xref>).</p>
            <p>Table <xref ref-type="table" rid="T2">2</xref> shows the composition of the individual samples including the animal species identified in the samples. The analyzed sample set consisting of hams included samples with a declared single animal species on the product label. Based on the results obtained from ten samples, we collected three samples which contained DNA of another animal species. Eight products were in line with labeling and identified animal species.</p>
            <table-wrap id="T2" position="float">
                <label>Table 2</label>
                <caption>
                    <p>Composition of samples and species identification.</p>
                </caption>
                <table frame="hsides" rules="none" width="100%">
                    <thead>
                        <tr>
                            <th>Sample</th>
                            <th>Fat (g)</th>
                            <th>Carbohydrates (g)</th>
                            <th>Protein (g)</th>
                            <th>Salt (g)</th>
                            <th>Meat content %</th>
                            <th>Cattle</th>
                            <th>Pork</th>
                            <th>Chicken</th>
                            <th>Turkey</th>
                        </tr>
                        <tr>
                            <th colspan="10">
                                <hr/>
                            </th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr align="center">
                            <td>1</td>
                            <td>9.0</td>
                            <td>1.0</td>
                            <td>20.0</td>
                            <td>2.75</td>
                            <td>96</td>
                            <td>-</td>
                            <td>X</td>
                            <td>-</td>
                            <td>-</td>
                        </tr>
                        <tr align="center">
                            <td>2</td>
                            <td>2.0</td>
                            <td>1.0</td>
                            <td>20.0</td>
                            <td>2.5</td>
                            <td>96</td>
                            <td>-</td>
                            <td>X</td>
                            <td>-</td>
                            <td>-</td>
                        </tr>
                        <tr align="center">
                            <td>3</td>
                            <td>3.0</td>
                            <td>1.0</td>
                            <td>20.0</td>
                            <td>2.5</td>
                            <td>96</td>
                            <td>-</td>
                            <td>X</td>
                            <td>-</td>
                            <td>-</td>
                        </tr>
                        <tr align="center">
                            <td>4</td>
                            <td>4.1</td>
                            <td>&#x003C;0.5</td>
                            <td>19.5</td>
                            <td>2.22</td>
                            <td>96</td>
                            <td>-</td>
                            <td>X</td>
                            <td>-</td>
                            <td>-</td>
                        </tr>
                        <tr align="center">
                            <td>5</td>
                            <td>3.0</td>
                            <td>0.5</td>
                            <td>19.5</td>
                            <td>1.9</td>
                            <td>94</td>
                            <td>-</td>
                            <td>X</td>
                            <td>-</td>
                            <td>-</td>
                        </tr>
                        <tr align="center">
                            <td>6</td>
                            <td>3.2</td>
                            <td>1.2</td>
                            <td>15.6</td>
                            <td>2.1</td>
                            <td>85</td>
                            <td>-</td>
                            <td>X</td>
                            <td>-</td>
                            <td>-</td>
                        </tr>
                        <tr align="center">
                            <td>7</td>
                            <td>3.2</td>
                            <td>1.2</td>
                            <td>15.6</td>
                            <td>2.1</td>
                            <td>85</td>
                            <td>-</td>
                            <td>X</td>
                            <td>X</td>
                            <td>X</td>
                        </tr>
                        <tr align="center">
                            <td>8</td>
                            <td>4.0</td>
                            <td>0.3</td>
                            <td>14.3</td>
                            <td>2.1</td>
                            <td>75</td>
                            <td>-</td>
                            <td>X</td>
                            <td>-</td>
                            <td>-</td>
                        </tr>
                        <tr align="center">
                            <td>9</td>
                            <td>2.0</td>
                            <td>1.0</td>
                            <td>20.0</td>
                            <td>2.25</td>
                            <td>95</td>
                            <td>-</td>
                            <td>X</td>
                            <td>-</td>
                            <td>-</td>
                        </tr>
                        <tr align="center">
                            <td>10</td>
                            <td>8.0</td>
                            <td>1.4</td>
                            <td>19.1</td>
                            <td>2.0</td>
                            <td>90</td>
                            <td>X</td>
                            <td>X</td>
                            <td>-</td>
                            <td>-</td>
                        </tr>
                    </tbody>
                </table>
                <table-wrap-foot>
                    <fn id="T2FN1">
                        <p>Note: X – Declared, - undeclared.</p>
                    </fn>
                </table-wrap-foot>
            </table-wrap>
            <p>Based on the EU recommendation (<xref ref-type="bibr" rid="r10">European Commission, 2013</xref>), a detection threshold of 1% (w/w) was targeted. Our results are consistent with studies done abroad. In a study carried out in Turkey, 73 samples of meat and meat products sold in shops, markets and public bazaars located in different urban areas in Istanbul were analyzed. The study pointed to a number of disagreements with the label on the product label (&#xD6;zpinar et al., 2013). A study on meat processing revealed that the DNA fragment size was progressively degraded into smaller fragments with increase in duration of heating and temperature (&#x15E;akalar et al., 2012). Species identification is important for legal authorities to detect undeclared ingredients in food products. When an undeclared species is detected, the next step is to discriminate between intentional substitution with cheaper meat or unintentional contamination during food preparation (<xref ref-type="bibr" rid="r8">Cravero et al., 2019</xref>). DNA Microarray as a method has been widely preferred for understanding mechanisms, detection of foodborne microbial pathogens and food safety studies, nutreaceuticals and functional foods as well as following up the different expression levels of DNA in bacteria, yeasts, plants and human; genetic and mutation analyses; environmental studies; identification of antimicrobial genes, proteomics, protein-nucleic acids, protein-protein interactions, biochemical analysis of protein functions and drug development (<xref ref-type="bibr" rid="r5">Bottero and Dalmasso, 2010;</xref> <xref ref-type="bibr" rid="r18">Kostrzynska and Bachand, 2006</xref>).</p>
        </sec>
        <sec sec-type="conclusion">
            <title>CONCLUSION</title>
            <p>From the obtained results we can conclude that it is necessary to put emphasis on intensive control and management of technological steps in the production of meat products. In the analyzed samples, we captured 2 samples that did not conform to the label on the product label. DNA of other species was also detected in the samples. The presence of bovine and poultry DNA is explained by the fact that some manufacturers may have added bovine haemoglobin or poultry globin to improve product colour or it may be contamination. The results obtained are an incentive for further investigation and analysis.</p>
        </sec>
    </body>
    <back>
        <ack>
            <title>Acknowledgments:</title>
            <p>This work was supported by grant VEGA No. 1/0276/18. This work was supported by the Slovak Research and Development Agency under the contract no. APVV-17-
0508.</p>
        </ack>
        <ref-list>
            <ref id="r1">
                <label>1</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Azuka</surname>
                            <given-names>I.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Ingrid</surname>
                            <given-names>H.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Georg</surname>
                            <given-names>H.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Andreas</surname>
                            <given-names>M.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Ulrich</surname>
                            <given-names>B.</given-names>
                        </name>
                    </person-group>
                    <article-title>Biochip technology for the detection of animal species in meat products.</article-title>
                    <source>
                        <italic>Food Analytical Methods</italic>
                    </source>
                    <year>2011</year>
                    <volume>4</volume>
                    <issue>3</issue>
                    <fpage>389</fpage>
                    <lpage>398</lpage>
                    <pub-id pub-id-type="doi">10.1007/s12161-010-9178-9</pub-id>
                </element-citation>
            </ref>
            <ref id="r2">
                <label>2</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Barai</surname>
                            <given-names>B.K.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Nazak</surname>
                            <given-names>R.R.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Singhal</surname>
                            <given-names>R.S.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Kulkarni</surname>
                            <given-names>P.R.</given-names>
                        </name>
                    </person-group>
                    <article-title>Approaches to the detection of meat adulteration.</article-title>
                    <source>
                        <italic>Trends in Food Science and Technology.</italic>
                    </source>
                    <year>1992</year>
                    <volume>3</volume>
                    <fpage>69</fpage>
                    <lpage>72</lpage>
                    <pub-id pub-id-type="doi">10.1016/0924-2244(92)90133-H</pub-id>
                </element-citation>
            </ref>
            <ref id="r3">
                <label>3</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Bertolini</surname>
                            <given-names>F.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Ghionda</surname>
                            <given-names>M.C.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>D'Alessandro</surname>
                            <given-names>D.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Geraci</surname>
                            <given-names>C.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Chiofalo</surname>
                            <given-names>V.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Fontanesi</surname>
                            <given-names>L.</given-names>
                        </name>
                    </person-group>
                    <article-title>A Next Generation Semiconductor Based Sequencing Approach for the Identification of Meat Species in DNA Mixtures.</article-title>
                    <source>
                        <italic>PLoS One</italic>
                    </source>
                    <year>2015</year>
                    <volume>10</volume>
                    <issue>4</issue>
                    <elocation-id>e0121701</elocation-id>
                    <pub-id pub-id-type="doi">10.1371/journal.pone.0121701</pub-id>
                </element-citation>
            </ref>
            <ref id="r4">
                <label>4</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Bonany</surname>
                            <given-names>J.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Brugger</surname>
                            <given-names>C.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Buehler</surname>
                            <given-names>A.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Carb'o</surname>
                            <given-names>J.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Codarin</surname>
                            <given-names>S.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Donati</surname>
                            <given-names>F.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Schoorl</surname>
                            <given-names>F.</given-names>
                        </name>
                    </person-group>
                    <article-title>Preference mapping of apple varieties in Europe.</article-title>
                    <source>
                        <italic>Food Quality and Preference</italic>
                    </source>
                    <year>2014</year>
                    <volume>32</volume>
                    <fpage>317</fpage>
                    <lpage>329</lpage>
                    <pub-id pub-id-type="doi">10.1016/j.foodqual.2013.09.010</pub-id>
                </element-citation>
            </ref>
            <ref id="r5">
                <label>5</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Bottero</surname>
                            <given-names>M.T.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Dalmasso</surname>
                            <given-names>A.</given-names>
                        </name>
                    </person-group>
                    <article-title>Animal species identification in food products: Evolution of biomolecular methods.</article-title>
                    <source>
                        <italic>Veterinary Journal.</italic>
                    </source>
                    <year>2010</year>
                    <volume>190</volume>
                    <issue>1</issue>
                    <fpage>34</fpage>
                    <lpage>38</lpage>
                    <pub-id pub-id-type="doi">10.1016/j.tvjl.2010.09.024</pub-id>
                </element-citation>
            </ref>
            <ref id="r6">
                <label>6</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Cadena</surname>
                            <given-names>R.S.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Cruz</surname>
                            <given-names>A.G.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Faria</surname>
                            <given-names>J.A.F.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Bolini</surname>
                            <given-names>H.M.A.</given-names>
                        </name>
                    </person-group>
                    <article-title>Reduced fat and sugar vanilla ice creams: Sensory profiling and external preference mapping.</article-title>
                    <source>
                        <italic>Journal of Dairy Science.</italic>
                    </source>
                    <year>2012</year>
                    <volume>95</volume>
                    <fpage>4842</fpage>
                    <lpage>4850</lpage>
                    <pub-id pub-id-type="doi">10.3168/jds.2012-5526</pub-id>
                </element-citation>
            </ref>
            <ref id="r7">
                <label>7</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Carbonell</surname>
                            <given-names>L.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Izquierdo</surname>
                            <given-names>L.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Carbonell</surname>
                            <given-names>I.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Costell</surname>
                            <given-names>E.</given-names>
                        </name>
                    </person-group>
                    <article-title>Segmentation of food consumers according to their correlations with sensory attributes projected on preference spaces.</article-title>
                    <source>
                        <italic>Food Quality and Preference</italic>
                    </source>
                    <year>2008</year>
                    <volume>19</volume>
                    <issue>1</issue>
                    <fpage>71</fpage>
                    <lpage>78</lpage>
                    <pub-id pub-id-type="doi">10.1016/j.foodqual.2007.06.006</pub-id>
                </element-citation>
            </ref>
            <ref id="r8">
                <label>8</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Cravero</surname>
                            <given-names>D.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Cerutti</surname>
                            <given-names>F.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Maniaci</surname>
                            <given-names>M.G.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Barzanti</surname>
                            <given-names>P.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Scaramagli</surname>
                            <given-names>S.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Riina</surname>
                            <given-names>M.V.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Ingravalle</surname>
                            <given-names>F.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Acutis</surname>
                            <given-names>P.L.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Peletto</surname>
                            <given-names>S.</given-names>
                        </name>
                    </person-group>
                    <article-title>Evaluation of DNA isolation procedures from meat-based foods and development of a DNA quality score.</article-title>
                    <source>
                        <italic>LWT</italic>
                    </source>
                    <year>2019</year>
                    <volume>106</volume>
                    <fpage>64</fpage>
                    <lpage>71</lpage>
                    <pub-id pub-id-type="doi">10.1016/j.lwt.2019.02.028</pub-id>
                </element-citation>
            </ref>
            <ref id="r9">
                <label>9</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Engel</surname>
                            <given-names>E.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Nicklaus</surname>
                            <given-names>S.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Septier</surname>
                            <given-names>C.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Salles</surname>
                            <given-names>C.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Quéré</surname>
                            <given-names>J.L.</given-names>
                        </name>
                    </person-group>
                    <article-title>Evolution of the taste of a bitter Camembert cheese during ripening: characterization of a matrix effect.</article-title>
                    <source>
                        <italic>Journal of Agricultural and Food Chemistry</italic>
                    </source>
                    <year>2001</year>
                    <volume>49</volume>
                    <issue>6</issue>
                    <fpage>2930</fpage>
                    <lpage>2939</lpage>
                    <pub-id pub-id-type="doi">10.1021/jf000967m</pub-id>
                </element-citation>
            </ref>
            <ref id="r10">
                <label>10</label>
                <element-citation publication-type="web">
                    <person-group person-group-type="author">
                        <collab>European Commission</collab>
                    </person-group>
                    <year>2013</year>
                    <source>
                        <italic>Recommendation 2013/99/EU on a coordinated control plan with a view to establish the prevalence of fraudulent practices in the marketing of certain foods.</italic> Available at : http://eur-lex.europa.eu/legalcontent/EN/TXT/PDF/?uri=CELEX:32013H0099&#x26;qid=1429713190676&#x26;from=EN.
            </source>
                </element-citation>
            </ref>
            <ref id="r11">
                <label>11</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Gaze</surname>
                            <given-names>L.V.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Oliveira</surname>
                            <given-names>B.R.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Ferrao</surname>
                            <given-names>L.L.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Granato</surname>
                            <given-names>D.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Cavalcanti</surname>
                            <given-names>R.N.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Conte J'unior</surname>
                            <given-names>C.A.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Freitas</surname>
                            <given-names>M.Q.</given-names>
                        </name>
                    </person-group>
                    <article-title>Preference mapping of dulce de leche commercialized in Brazilian markets.</article-title>
                    <source>
                        <italic>Journal of Dairy Science.</italic>
                    </source>
                    <year>2015</year>
                    <volume>98</volume>
                    <fpage>1443</fpage>
                    <lpage>1454</lpage>
                    <pub-id pub-id-type="doi">10.3168/jds.2014-8470</pub-id>
                </element-citation>
            </ref>
            <ref id="r12">
                <label>12</label>
                <element-citation publication-type="book">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Greenhoff</surname>
                            <given-names>K.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>MacFie</surname>
                            <given-names>H.J.H.</given-names>
                        </name>
                    </person-group>
                    <source>Preference Mapping in practice. In MacFie, H. J. H., Thomson, D. M. H. (eds) <italic>Measurement of food preferences</italic>. Boston, Maryland : Springer, p. 137-166. ISBN 978-1-4613-5908-1.</source>
                    <year>1994</year>
                    <pub-id pub-id-type="doi">10.1007/978-1-4615-2171-6_6</pub-id>
                </element-citation>
            </ref>
            <ref id="r13">
                <label>13</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Günssen</surname>
                            <given-names>U.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Aydin</surname>
                            <given-names>A.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Ovali</surname>
                            <given-names>B.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Coskun</surname>
                            <given-names>Y.</given-names>
                        </name>
                    </person-group>
                    <article-title>Cig et ve ısıl islem görmüs set ürünlerinde ELISA teknigi ile farklı et türlerinin tespiti (Determination of different types of meat by using ELISA technique in cig meat and heat treated set products).</article-title>
                    <source>
                        <italic>Istanbul Üniv Vet Fak Derg.</italic>
                    </source>
                    <year>2006</year>
                    <volume>32</volume>
                    <fpage>45</fpage>
                    <lpage>52</lpage>
                    <comment>[in Turkish].</comment>
                </element-citation>
            </ref>
            <ref id="r14">
                <label>14</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Hsieh</surname>
                            <given-names>Y.H.P.</given-names>
                        </name>
                    </person-group>
                    <article-title>Meat species identification. Handbook of Food Science.</article-title>
                    <source>
                        <italic>Technology, and Engineering</italic>
                    </source>
                    <year>2006</year>
                    <volume>1</volume>
                    <fpage>6</fpage>
                    <pub-id pub-id-type="doi">10.1201/b15995-33</pub-id>
                </element-citation>
            </ref>
            <ref id="r15">
                <label>15</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>İlhak</surname>
                            <given-names>O.I.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Arslan</surname>
                            <given-names>A.</given-names>
                        </name>
                    </person-group>
                    <article-title>Identification of Meat Species by Polymerase Chain Reaction (PCR) Technique.</article-title>
                    <source>
                        <italic>Turkish Journal of Veterinary and Animal Sciences.</italic>
                    </source>
                    <year>2007</year>
                    <volume>31</volume>
                    <issue>3</issue>
                    <fpage>159</fpage>
                    <lpage>163</lpage>
                    <ext-link xlink:href="https://dergipark.org.tr/en/download/article-file/132512" xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri">https://dergipark.org.tr/en/download/article-file/132512</ext-link>
                </element-citation>
            </ref>
            <ref id="r16">
                <label>16</label>
                <element-citation publication-type="other">
                    <person-group person-group-type="author">
                        <collab>ISO 8589</collab>
                    </person-group>
                    <year>2007</year>
                    <source>
                        <italic>Sensory analysis — General guidance for the design of test rooms.</italic> International Standard Organisation</source>
                </element-citation>
            </ref>
            <ref id="r17">
                <label>17</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Jolliffe</surname>
                            <given-names>I.T.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Cadima</surname>
                            <given-names>J.</given-names>
                        </name>
                    </person-group>
                    <article-title>Principal component analysis: a review and recent developments. In Philosophical transactions.</article-title>
                    <source>
                        <italic>Series A, Mathematical, physical, and engineering sciences</italic>
                    </source>
                    <year>2016</year>
                    <volume>374</volume>
                    <issue>2065</issue>
                    <fpage>2015</fpage>
                    <lpage>2020</lpage>
                    <pub-id pub-id-type="doi">10.1098/rsta.2015.0202</pub-id>
                </element-citation>
            </ref>
            <ref id="r18">
                <label>18</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Kostrzynska</surname>
                            <given-names>M.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Bachand</surname>
                            <given-names>A.</given-names>
                        </name>
                    </person-group>
                    <article-title>Application of DNA microarray technology for detection, identification, and characterization of food-borne pathogens.</article-title>
                    <source>
                        <italic>Canadian Journal of Microbiology</italic>
                    </source>
                    <year>2006</year>
                    <volume>52</volume>
                    <issue>1</issue>
                    <fpage>1</fpage>
                    <lpage>8</lpage>
                    <pub-id pub-id-type="doi">10.1139/w05-105</pub-id>
                </element-citation>
            </ref>
            <ref id="r19">
                <label>19</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>MacFie</surname>
                            <given-names>H.</given-names>
                        </name>
                    </person-group>
                    <article-title>Preference mapping and food product development.</article-title>
                    <source>
                        <italic>Consumerled Food Product Development</italic>
                    </source>
                    <year>2007</year>
                    <volume>2007</volume>
                    <fpage>551</fpage>
                    <lpage>592</lpage>
                    <pub-id pub-id-type="doi">10.1533/9781845693381.3.551</pub-id>
                </element-citation>
            </ref>
            <ref id="r20">
                <label>20</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Mansoor</surname>
                            <given-names>B.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Mohamad</surname>
                            <given-names>J.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Heena</surname>
                            <given-names>Para.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Parveez</surname>
                            <given-names>A. B.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Syed</surname>
                            <given-names>G.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Subha</surname>
                            <given-names>B.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Asif</surname>
                            <given-names>W.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Rajesh</surname>
                            <given-names>Q.</given-names>
                        </name>
                    </person-group>
                    <article-title>Fraudulent Adulteration Substitution of Meat.</article-title>
                    <source>
                        <italic>Journal of Recent Research and Applied Studies</italic>
                    </source>
                    <year>2015</year>
                    <volume>2</volume>
                    <fpage>22</fpage>
                    <lpage>33</lpage>
                </element-citation>
            </ref>
            <ref id="r21">
                <label>21</label>
                <element-citation publication-type="book">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Meullenet</surname>
                            <given-names>J.F.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Xiong</surname>
                            <given-names>R.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Findlay</surname>
                            <given-names>C.</given-names>
                        </name>
                    </person-group>
                    <source>
                        <italic>Multivariate and probabilistic analyses of sensory science problems</italic>. Ames, IA : IFT Press. Blackwell Publishing, 256 p. ISBN 978-0-813-80178-0.</source>
                    <year>2007</year>
                </element-citation>
            </ref>
            <ref id="r22">
                <label>22</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Miller</surname>
                            <given-names>M.B.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Tang</surname>
                            <given-names>Y.W.</given-names>
                        </name>
                    </person-group>
                    <article-title>Basic concepts of mikroarrays and potential applications in clinical microbiology.</article-title>
                    <source>
                        <italic>Clin. Microbiol. Rev.</italic>
                    </source>
                    <year>2009</year>
                    <volume>22</volume>
                    <issue>4</issue>
                    <fpage>611</fpage>
                    <lpage>633</lpage>
                    <pub-id pub-id-type="doi">10.1128/CMR.00019-09</pub-id>
                </element-citation>
            </ref>
            <ref id="r23">
                <label>23</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Montowska</surname>
                            <given-names>M.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Rao</surname>
                            <given-names>W.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Alexander</surname>
                            <given-names>M.R.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Tucker</surname>
                            <given-names>G.A.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Barett</surname>
                            <given-names>D.A.</given-names>
                        </name>
                    </person-group>
                    <article-title>Tryptic digestion coupled with ambient desorption electrospray ionization and liquid extraction surface analysis mass spectrometry enabling identification of skeletal muscle proteins in mixtures and distinguishing between beef, pork, horse, chicken, and Turkey meat.</article-title>
                    <source>
                        <italic>Analytical Chemistry.</italic>
                    </source>
                    <year>2014</year>
                    <volume>6</volume>
                    <fpage>4479</fpage>
                    <lpage>4487</lpage>
                    <pub-id pub-id-type="doi">10.1021/ac5003432</pub-id>
                </element-citation>
            </ref>
            <ref id="r24">
                <label>24</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Myers</surname>
                            <given-names>M.J.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Farrell</surname>
                            <given-names>D.E.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Deaver</surname>
                            <given-names>C.M.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Mason</surname>
                            <given-names>J.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Swaim</surname>
                            <given-names>H.L.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Yancy</surname>
                            <given-names>H.F.</given-names>
                        </name>
                    </person-group>
                    <article-title>Detection of rendered meat and bone meals by PCR is dependent on animal species of origin and DNA extraction method.</article-title>
                    <source>
                        <italic>Journal of Food Protection.</italic>
                    </source>
                    <year>2010</year>
                    <volume>73</volume>
                    <issue>6</issue>
                    <fpage>1090</fpage>
                    <lpage>1096</lpage>
                    <pub-id pub-id-type="doi">10.4315/0362-028x-73.6.1090</pub-id>
                </element-citation>
            </ref>
            <ref id="r25">
                <label>25</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Oltman</surname>
                            <given-names>A.E.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Yates</surname>
                            <given-names>M.D.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Drake</surname>
                            <given-names>M.A.</given-names>
                        </name>
                    </person-group>
                    <article-title>Preference mapping of fresh tomatoes across 3 stages of consumption.</article-title>
                    <source>
                        <italic>Journal of Food Science</italic>
                    </source>
                    <year>2016</year>
                    <volume>81</volume>
                    <fpage>1495</fpage>
                    <lpage>1505</lpage>
                    <pub-id pub-id-type="doi">10.1111/1750-3841.13306</pub-id>
                </element-citation>
            </ref>
            <ref id="r26">
                <label>26</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Özpinar</surname>
                            <given-names>H.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Tezmen</surname>
                            <given-names>G.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Gökçe</surname>
                            <given-names>I.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Tekiner</surname>
                            <given-names>I.H.</given-names>
                        </name>
                    </person-group>
                    <article-title>Detection of Animal Species in Some Meat and Meat Products by Comparatively Using DNA Microarray and Real Time PCR Methods.</article-title>
                    <source>
                        <italic>Kafkas Üniversitesi Veteriner Fakültesi Dergisi.</italic>
                    </source>
                    <year>2013</year>
                    <volume>19</volume>
                    <issue>2</issue>
                    <fpage>245</fpage>
                    <lpage>252</lpage>
                    <pub-id pub-id-type="doi">10.9775/kvfd.2012.7616</pub-id>
                </element-citation>
            </ref>
            <ref id="r27">
                <label>27</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Pereira</surname>
                            <given-names>F.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Carneiro</surname>
                            <given-names>J.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Amorim</surname>
                            <given-names>A.</given-names>
                        </name>
                    </person-group>
                    <article-title>Identification of species with DNAbased technology: current progress and challenges.</article-title>
                    <source>
                        <italic>Recent Pat. DNA Gene. Seq.</italic>
                    </source>
                    <year>2008</year>
                    <volume>2</volume>
                    <issue>3</issue>
                    <fpage>187</fpage>
                    <lpage>199</lpage>
                    <pub-id pub-id-type="doi">10.2174/187221508786241738</pub-id>
                </element-citation>
            </ref>
            <ref id="r28">
                <label>28</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Şakalar</surname>
                            <given-names>E.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Abasiyanik</surname>
                            <given-names>M.F.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Bektik</surname>
                            <given-names>E.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Tayyrov</surname>
                            <given-names>A.</given-names>
                        </name>
                    </person-group>
                    <article-title>Effect of heat processing on DNA quantification of meat species.</article-title>
                    <source>
                        <italic>Journal of Food Science</italic>
                    </source>
                    <year>2012</year>
                    <volume>77</volume>
                    <issue>9</issue>
                    <fpage>N40</fpage>
                    <lpage>N44</lpage>
                    <pub-id pub-id-type="doi">10.1111/j.1750-3841.2012.02853.x</pub-id>
                </element-citation>
            </ref>
            <ref id="r29">
                <label>29</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Santillo</surname>
                            <given-names>A.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Albenzio</surname>
                            <given-names>M.</given-names>
                        </name>
                    </person-group>
                    <article-title>Sensory Profile and Consumers' Liking of Functional Ovine Cheese.</article-title>
                    <source>
                        <italic>Foods</italic>
                    </source>
                    <year>2015</year>
                    <volume>4</volume>
                    <issue>4</issue>
                    <fpage>665</fpage>
                    <lpage>677</lpage>
                    <pub-id pub-id-type="doi">10.3390/foods4040665</pub-id>
                </element-citation>
            </ref>
            <ref id="r30">
                <label>30</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Supeková</surname>
                            <given-names>S.</given-names>
                        </name>
                    </person-group>
                    <article-title>Kvalita potravín a "značka kvality SK" z pohľadu spotrebiteľa (Food quality and "SK quality label" from the consumer perspective.).</article-title>
                    <source>
                        <italic>Trendy v potravinárstve</italic>
                    </source>
                    <year>2008</year>
                    <volume>15</volume>
                    <issue>2</issue>
                    <fpage>5</fpage>
                    <lpage>6</lpage>
                </element-citation>
            </ref>
            <ref id="r31">
                <label>31</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Villamor</surname>
                            <given-names>R.R.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Daniels</surname>
                            <given-names>C.H.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Moore</surname>
                            <given-names>P.P.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Ross</surname>
                            <given-names>C.F.</given-names>
                        </name>
                    </person-group>
                    <article-title>Preference mapping of frozen and fresh raspberries.</article-title>
                    <source>
                        <italic>Journal of Food Science</italic>
                    </source>
                    <year>2013</year>
                    <volume>78</volume>
                    <fpage>911</fpage>
                    <lpage>919</lpage>
                    <pub-id pub-id-type="doi">10.1111/1750-3841.12125</pub-id>
                </element-citation>
            </ref>
            <ref id="r32">
                <label>32</label>
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Yosef</surname>
                            <given-names>T.A.</given-names>
                        </name>
                    </person-group>
                    <article-title>Food Forensics: Using DNA-Based Technology for the Detection of Animal Species in Meat Products.</article-title>
                    <source>
                        <italic>Nature and Science.</italic>
                    </source>
                    <year>2014</year>
                    <volume>12</volume>
                    <fpage>82</fpage>
                    <lpage>90</lpage>
                </element-citation>
            </ref>
        </ref-list>
    </back>
</article>	