<?xml version="1.0" encoding="utf-8" ?>
<article xml:lang="en" article-type="research-article" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">
    <front>
        <journal-meta>
            <journal-id journal-id-type="publisher-id">PSJFS</journal-id>
            <journal-title-group>
                <journal-title>Potravinarstvo Slovak Journal of Food Sciences</journal-title>
                <abbrev-journal-title abbrev-type="pubmed">Potr. S. J. F. Sci.</abbrev-journal-title>
            </journal-title-group>
            <issn pub-type="ppub">1338-0230</issn>
            <issn pub-type="epub">1337-0960</issn>
            <publisher>
                <publisher-name>Association HACCP Consulting</publisher-name>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="publisher-id">PSJFS-13-1-438</article-id>
            <article-id pub-id-type="doi">10.5219/1115</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>ARTICLE</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>THE EFFECT OF MELON AND WATERMELON CONCENTRATES ON CONSUMER PROPERTIES OF POLYCOMPONENT DAIRY DESSERT</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-8683-8159</contrib-id>
                    <name>
                        <surname>Gorlov</surname>
                        <given-names>Ivan Fyodorovich</given-names>
                    </name>
                    <xref ref-type="corresp" rid="cor1">&#x002A;</xref>
                </contrib>
                <contrib contrib-type="author">
                    <name>
                        <surname>Mgebrishvili</surname>
                        <given-names>Irina Vazhayena</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff2" />
                </contrib>
                <contrib contrib-type="author">
                    <contrib-id contrib-id-type="orcid">http://orcid.org/0000-0001-9542-5893</contrib-id>
                    <name>
                        <surname>Slogenkina</surname>
                        <given-names>Marina Ivanovna</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff3" />
                </contrib>
                <contrib contrib-type="author">
                    <name>
                        <surname>Mosolova</surname>
                        <given-names>Natalya Ivanovna</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff4" />
                </contrib>
                <contrib contrib-type="author">
                    <name>
                        <surname>Tarasova</surname>
                        <given-names>Irina Alexandrovna</given-names>
                    </name>
                    <xref ref-type="aff" rid="aff5" />
                </contrib>
                <aff id="aff2">
                    <institution>Irina Vazhayena Mgebrishvili, Volgograd State Technical University, Lenin Avenue 28, 400050 Volgograd, Russia, Tel. +79610789402, E-mail: ira-06@inbox.ru</institution>
                </aff>
                <aff id="aff3">
                    <institution>Marina Ivanovna Slozhenkina, Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production, Rokossovsky Str., 6, Volgograd, 400131 Russia; Volgograd State Technical University, Lenin Avenue 28, 400050 Volgograd, Russia, Tel: +79047729999, E-mail: niimmp@mail.ru</institution>
                </aff>
                <aff id="aff4">
                    <institution>Natalya Ivanovna Mosolova, Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production, Rokossovsky Str. 6, Volgograd, 400131 Russia, Tel.: +79033735182, E-mail: natalyniimmp@mail.ru</institution>
                </aff>
                <aff id="aff5">
                    <institution>Irina Alexandrovna Tarasova, Volgograd State Technical University, Lenin Avenue 28, 400050 Volgograd, Russia, Tel. +79272581659, E-mail: irinka_ta@mail.ru</institution>
                </aff>
            </contrib-group>
            <author-notes>
                <corresp id="cor1">
                    <label>&#x002A;</label>Corresponding author: Ivan Fyodorovich Gorlov, Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production, Rokossovsky Str., 6, Volgograd, 400131 Russia; Volgograd State Technical University, Lenin Avenue 28, 400050 Volgograd, Russia, Tel: <phone>+78442391048</phone>, E-mail: <email xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="nniimmp@mail.ru"></email></corresp>
            </author-notes>
            <pub-date pub-type="ppub">
                <month>1</month>
                <year>2019</year>
            </pub-date>
            <volume>13</volume>
            <issue>1</issue>
            <fpage>438</fpage>
            <lpage>442</lpage>
            <history>
                <date date-type="received">
                    <day>4</day>
                    <month>4</month>
                    <year>2019</year>
                </date>
                <date date-type="accepted">
                    <day>26</day>
                    <month>4</month>
                    <year>2019</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>&#x00A9; Association HACCP Consulting. All rights reserved.</copyright-statement>
                <copyright-year>2019</copyright-year>
                <license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">
                    <license-p>This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<uri xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0</uri>) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <abstract>
                <p>A full balanced nutrition is a necessary condition for a person&#x2019;s normal physical and mental development, resistance to the effects of adverse environmental factors and strengthening immunity, which is of particular importance in the bad ecological situation in the world. Providing the population with high-quality bio-functional food is an important state task, the fulfillment of which is the key to the health of the nation, and ultimately ensures the security of the country. One of the effective ways to prevent and treat various diseases is the development of a new generation of functional dessert products with dietary properties. As a basis, there has been a principle considered, i.e., the high nutritional, biological value and physiological activity of the product are predetermined by the high quality of the raw materials. The article analyzes the state of the dairy industry at the present stage. The efficiency of melon and watermelon concentrates in the production of multicomponent dairy dessert has been substantiated. The positive effect of the tested concentrates on the structural and mechanical properties of the jelly part of the dessert, organoleptic characteristics and nutritional value of the product has been established. The optimal concentration of the gelling agent in the jelly has been determined. There were some conducted experiments aimed at maximum reducing of the gelling agent&#x2019;s weight fraction in the product.</p>
            </abstract>
            <kwd-group>
                <kwd>milk dessert</kwd>
                <kwd>whey</kwd>
                <kwd>jelly</kwd>
                <kwd>carrageenan</kwd>
                <kwd>melon and watermelon</kwd>
            </kwd-group>
        </article-meta>
    </front>
    <body>
        <sec sec-type="intro">
            <title>INTRODUCTION</title>
            <p>At the present stage of the food industry development, the whey as the main raw material has been a research subject for a number of scientific schools in the USA, Great Britain and Japan (<xref ref-type="bibr" rid="b7">Faryabi et al., 2009;</xref> <xref ref-type="bibr" rid="b24">Yang, Irudayaraj and Sakhamuri, 2001</xref>). Their research studies were mainly aimed at functional properties of whey and its feasibility, mainly in clinical nutrition (<xref ref-type="bibr" rid="b8">Ghosh and Playford, 2003</xref>). Whey proteins are used to prepare baby foods, since their composition is more similar to the composition of mother milk proteins (<xref ref-type="bibr" rid="b11">Gorlov et al., 2015</xref>).</p>
            <p>Today, one of the promising areas for the introduction of whey products is the market for dairy desserts. It is considered to be one of the most dynamically developing and marginal. Over the four pre-crisis years, it grew by more than 30% (<xref ref-type="bibr" rid="b20">Mgebrishvili et al., 2013a</xref>). The reason for this was not only the high demand for dairy desserts, but also the constantly expanding assortment. Original milk desserts enjoy high demand, their consumer properties are formed depending on the type and quality of the raw materials used (<xref ref-type="bibr" rid="b17">Khramtsov and Vasilisin, 2004</xref>). In the Lower Volga region, there are considerable regional resources of watermelon and melon that may be involved in the production of dairy products with a predetermined composition and consumer properties (<xref ref-type="bibr" rid="b19">Mgebrishvili and Gorlov, 2012</xref>). In solving this problem, a promising direction is the development of innovative functional complex dairy desserts with high nutritional and biological value.</p>
            <p>The combined use of by-products of dairy processing production and regional plant raw materials offers exciting possibilities for creating new types of dairy products with preventive properties, provided their cost is reduced (<xref ref-type="bibr" rid="b21">Mgebrishvili et al., 2013b</xref>).</p>
            <p>The research conducted meets the current trends in the development of the dairy industry. The most important trend is the development of innovative technologies aimed at expanding the range and improving the quality of output products. An important aspect here is the use of secondary dairy raw materials, in particular, whey, which not only increases the biological value of the product, but also reduces its cost price (<xref ref-type="bibr" rid="b10">Gorlov, Mosolova nad Korotkova, 2012</xref>). In relation to the problem, it becomes appropriate to use melon and watermelon concentrates as functional fillers in recipes for dairy desserts. New types of fillers have many useful properties, which makes the dessert functional.</p>
            <sec>
                <title>Scientific hypothesis</title>
                <p>We are expecting: watermelon and melon fillers has significant effect on the process of structure formation.</p>
            </sec>
        </sec>
        <sec sec-type="materials|methods">
            <title>MATERIAL AND METHODOLOGY</title>
            <p>The research material contained raw ingredients, namely, goat cheese, unsalted cheese whey, watermelon (<italic>Citr&#xFA;llus lan&#xE1;tus</italic>), melon (<italic>Cucumis melo</italic>), sponge cake with flaxseed and produced milk dessert itself.</p>
            <p>Evaluation of the quality of the raw materials and product was conducted according to the following generally accepted methods, i.e., titrable acidity by the titrimetric method according to <xref ref-type="bibr" rid="b12">GOST 3624-92;</xref> density by the areometric method according to <xref ref-type="bibr" rid="b14">GOST R 54758-2011;</xref> and weight fraction of dry matter by the drying method according to <xref ref-type="bibr" rid="b13">GOST 3626-73</xref>. The optimal dose of the thickener in the jelly part of the dessert was established on the basis of the system&#x2019;s viscosity data obtained by the method of viscometry using an SV-100 vibration viscometer. With respect to the task, a low-cost technology for the production of melon and watermelon concentrates based on the thickening method was proposed. This technology allowed promising use of inexpensive plant material in recipes of complex dairy products.</p>
            <p>Milk dessert &#x22;Sankarini&#x22; consisted of three layers. The first layer was pieces of airy sponge cake enriched with flax seed additive (<xref ref-type="bibr" rid="b6">Edwards, 2009</xref>). The implementation of the agenda aimed at ensuring healthy nutrition of the population in the country made scientists pay attention to flax seed as a source of biologically active substances (<xref ref-type="bibr" rid="b4">Buldakov, 2001</xref>).</p>
            <p>The second layer was a jelly layer made of whey divided into two figured parts (<xref ref-type="bibr" rid="b5">Crus, Tinyakov and Fofanov, 1986</xref>). One part was filled with watermelon, the other one with melon. The color of the jelly was due to the natural pigments of the filler, i.e., concentrated mashed watermelon or melon pulp, condensed in the process of vacuum evaporation. Desalted whey was used as a base for jelly. Carrageenan that is a polysaccharide derived from red seaweed was used as a gelling agent (<xref ref-type="bibr" rid="b9">Gomes et al., 2013</xref>). Pieces of the jelly phase had a delicate sweet taste with the aroma characteristic of watermelon or melon, respectively.</p>
            <p>The third layer was the so-called “cheese cream” made from soft goat cheese (<xref ref-type="bibr" rid="b18">Krupin, 2009</xref>). The functional properties of this component were confirmed by the well-known effect of goat milk that is inherently identical to female milk, as it contains a lot of &#x3B2;-casein. Goat milk contains more sialic acid that is included in the structure of the body&#x27;s immunity barriers. The fat globules in goat milk are much smaller than in cow&#x27;s milk, therefore they are better digested by the body (<xref ref-type="bibr" rid="b22">Mgebrishvili et al., 2013c</xref>).</p>
            <p>The textures and tastes of all three parts of the product were brilliantly combined with the undoubted functionality of both individual ingredients and the whole Sankarini dessert (<xref ref-type="bibr" rid="b1">Ajewole et al., 1999</xref>). To analyze the effect of watermelon and melon concentrates in the production of a multicomponent dairy dessert based on whey, it was necessary to investigate changes in the structural and mechanical properties of jelly depending on the number of thickener doses applied.</p>
            <sec>
                <title>Statistic analysis</title>
                <p>Experimental studies presented in the article are processed by methods of variation statistics with the determination of the criterion of reliability of the difference according to the t-test Fisher criterion using the program STATISTIKA-10</p>
            </sec>
        </sec>
        <sec sec-type="results|discussion">
            <title>RESULTS AND DISCUSSION</title>
            <p>At the first stage of experimental studies, three laboratory samples of jelly without fillers were obtained. The recommended amount of carrageenan needed for gelling varies in over the range of 0.02 – 0.1% (<xref ref-type="bibr" rid="b3">Basiria et al., 2018</xref>).</p>
            <p>The proportion of gelling agent in the samples obtained was 0.05, 0.075 and 0.1%. Dry carrageenan was injected into the whey. One hour later, which was required for gelling, the dynamic viscosity of each sample was determined. According to the data obtained, the jelly samples with a carrageenan concentration of 0.05 and 0.075% at a temperature of about 20 &#xB0;C had a coefficient of dynamic viscosity of water of 0.15 Pa.s, that is, they were not defined as a viscous system. So, only the sample with a concentration of 0.1% carrageenan was identified as a viscous system.</p>
            <p>Today, scientists in different countries found that watermelon and melon contain high levels of fiber and pectin (<xref ref-type="bibr" rid="b2">Balaghi and Senge, 2014;</xref> <xref ref-type="bibr" rid="b23">Sethi et al., 2016;</xref> <xref ref-type="bibr" rid="b15">Gul et al., 2016;</xref> <xref ref-type="bibr" rid="b16">Karnopp et al., 2017</xref>). In this regard, it was suggested that using watermelon and melon concentrates as fillers would not only add preventive properties to the product, but also minimize the concentration of carrageenan and also make maximum use of secondary raw materials.</p>
            <p>At the second stage of the experimental studies, two model samples of jelly were developed, i.e., with watermelon and melon concentrates. The concentration of carrageenan in these samples was reduced to 0.075%. The values of the dynamic viscosity coefficients were obtained and best corresponded the jelly structure (Table <xref ref-type="table" rid="T1">1</xref>).</p>
            <table-wrap id="T1" position="float">
                <label>Table 1</label>
                <caption>
                    <p>Dynamic viscosity of jelly with fillers, with carrageenan concentration being of 0.075%</p>
                </caption>
                <table frame="hsides" rules="none" width="100%">
                    <thead>
                        <tr>
                            <th rowspan="3">Experience #</th>
                            <th rowspan="3">Sample&#x2019;s temperature, &#x00B0;C</th>
                            <th colspan="2">Dynamic viscosity coefficient [eta], Pa.s</th>
                        </tr>
                        <tr>
                            <th colspan="2">
                                <hr/>
                            </th>
                        </tr>
                        <tr>
                            <th>melon jelly</th>
                            <th>watermelon jelly</th>
                        </tr>
                        <tr>
                            <th colspan="4">
                                <hr/>
                            </th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr align="center">
                            <td>1</td>
                            <td>6</td>
                            <td>8.9</td>
                            <td>8.6</td>
                        </tr>
                        <tr align="center">
                            <td>2</td>
                            <td>9</td>
                            <td>8.54</td>
                            <td>8.2</td>
                        </tr>
                        <tr align="center">
                            <td>3</td>
                            <td>12</td>
                            <td>7.82</td>
                            <td>7.44</td>
                        </tr>
                        <tr align="center">
                            <td>4</td>
                            <td>15</td>
                            <td>7.36</td>
                            <td>6.98</td>
                        </tr>
                        <tr align="center">
                            <td>5</td>
                            <td>17</td>
                            <td>6.9</td>
                            <td>6.66</td>
                        </tr>
                        <tr align="center">
                            <td>6</td>
                            <td>20</td>
                            <td>6.2</td>
                            <td>6</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>When comparing the values of the dynamic viscosity coefficients for jelly samples without melons concentrates and with the concentrates, it could be seen that the fillers helped not only reduce the carrageenan concentration, but also increase the stability of the jelly system. These models are non-linear, statistically significant (F<sub>observe</sub> &#x3E; F<sub>table</sub>) high decreasing linear relationship and a good value of the determination coefficient, characterizing a high proportion of the variance of the effective feature (viscosity), can be explained by regression to the total variance. Show almost the same close linear relationship and viscosity rate. (Figure <xref ref-type="fig" rid="F1">1</xref>).</p>
            <fig id="F1" position="float">
                <label>Figure 1</label>
                <caption>
                    <p>Influence of watermelon and melon fillers on the viscosity of jelly samples with a concentration of 0.075% carrageenan.</p>
                </caption>
                <graphic xlink:href="PSJFS-13-1-438_F1.jpg"/>
            </fig>
            <p>At the third stage of the experimental studies, to minimize the concentration of the gelling agent, locust bean gum was proposed for this purpose. This type of stabilizers in combination with carrageenan exhibits synergistic properties and enhances the effect of the latter. Similarly, to the previous test, two model samples of jelly were developed: with watermelon and melon concentrates. The gelling agents&#x2019; (carrageenan and locust bean gum) concentrations in these samples were reduced to 0.025%. These models are also linear and statistically significant (F<sub>observe</sub> &#x3E; F<sub>table</sub>) with a high decreasing linear relationship and a good value of the determination coefficient characterizing the high proportion of the variance of the resultant trait (viscosity), explained by regression, in the total variance. Show almost the same close linear relationship and viscosity rate.</p>
            <p>The values of the dynamic viscosity coefficients were obtained and best corresponded to the jelly structure (Table <xref ref-type="table" rid="T2">2</xref>). When comparing the values of the dynamic viscosity coefficients for jelly samples with a carrageenan concentration of 0.075% and a gelation mixture concentration of 0.025%, it was clear that the data from the two experiments were almost identical with a considerable decrease in the proportion of gelling agent in the second case (Figure <xref ref-type="fig" rid="F2">2</xref>).</p>
            <fig id="F2" position="float">
                <label>Figure 2</label>
                <caption>
                    <p>Effect of watermelon and melon fillers on the viscosity of jelly samples with the concentration of the mixture of gelling agents 0.025%.</p>
                </caption>
                <graphic xlink:href="PSJFS-13-1-438_F2.jpg"/>
            </fig>
            <table-wrap id="T2" position="float">
                <label>Table 2</label>
                <caption>
                    <p>The coefficients of dynamic viscosity jelly with fillers, with gelling mixture concentration being of 0.025%.</p>
                </caption>
                <table frame="hsides" rules="none" width="100%">
                    <thead>
                        <tr>
                            <th rowspan="3">Experience #</th>
                            <th rowspan="3">Sample&#x2019;s temperature, &#x00B0;C</th>
                            <th colspan="2">Dynamic viscosity coefficient [eta], Pa.s</th>
                        </tr>
                        <tr>
                            <th colspan="2">
                                <hr/>
                            </th>
                        </tr>
                        <tr>
                            <th>melon jelly</th>
                            <th>watermelon jelly</th>
                        </tr>
                        <tr>
                            <th colspan="4">
                                <hr/>
                            </th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr align="center">
                            <td>1</td>
                            <td>6</td>
                            <td>9.26</td>
                            <td>9.02</td>
                        </tr>
                        <tr align="center">
                            <td>2</td>
                            <td>9</td>
                            <td>8.6</td>
                            <td>8.44</td>
                        </tr>
                        <tr align="center">
                            <td>3</td>
                            <td>12</td>
                            <td>7.9</td>
                            <td>7.66</td>
                        </tr>
                        <tr align="center">
                            <td>4</td>
                            <td>15</td>
                            <td>7.46</td>
                            <td>7.34</td>
                        </tr>
                        <tr align="center">
                            <td>5</td>
                            <td>17</td>
                            <td>7.1</td>
                            <td>6.86</td>
                        </tr>
                        <tr align="center">
                            <td>6</td>
                            <td>20</td>
                            <td>6.4</td>
                            <td>6.2</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>At the fourth stage of the experimental studies, an organoleptic assessment of the jelly phase of the milk dessert was made in terms of appearance, color, smell and texture. The objects of the study were model samples of jelly with watermelon and melon fillers obtained using a mixture of carrageenan and locust bean gum in an amount of 0.025% (Table <xref ref-type="table" rid="T3">3</xref>).At the fifth stage of the experimental studies, the physicochemical parameters of the jelly part of the dessert, in particular the acidity and weight fraction of dry substances, were determined. For this purpose, four laboratory samples of jelly were obtained, two with watermelon and two with melon fillers. Physicochemical characteristics of the jelly samples are shown in Table <xref ref-type="table" rid="T4">4</xref>.</p>
            <table-wrap id="T3" position="float">
                <label>Table 3</label>
                <caption>
                    <p>Organoleptic characteristics of the dessert’s jelly part.</p>
                </caption>
                <table frame="hsides" rules="none" width="100%">
                    <thead>
                        <tr>
                            <th>Indicator</th>
                            <th>Feature</th>
                        </tr>
                        <tr>
                            <th colspan="2">
                                <hr/>
                            </th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr align="center">
                            <td align="left">Appearance</td>
                            <td>Figured transparent pieces with glitter, keeping the shape</td>
                        </tr>
                        <tr align="center">
                            <td align="left">Color</td>
                            <td>Pink, yellow, characteristic of fillers</td>
                        </tr>
                        <tr align="center">
                            <td align="left">Smell</td>
                            <td>Pleasant, characteristic of fillers</td>
                        </tr>
                        <tr align="center">
                            <td align="left">Consistency</td>
                            <td>Dense, resilient, gelatinous</td>
                        </tr>
                        <tr align="center">
                            <td align="left">Taste</td>
                            <td>Sweet, characteristic of fillers</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <table-wrap id="T4" position="float">
                <label>Table 4</label>
                <caption>
                    <p>Physical and chemical indicators of jelly samples.</p>
                </caption>
                <table frame="hsides" rules="none" width="100%">
                    <thead>
                        <tr>
                            <th rowspan="3" align="left">Indicator</th>
                            <th colspan="2" align="left">Feature</th>
                        </tr>
                        <tr>
                            <th colspan="2">
                                <hr/>
                            </th>
                        </tr>
                        <tr>
                            <th>melon jelly</th>
                            <th>watermelon jelly</th>
                        </tr>
                        <tr>
                            <th colspan="3">
                                <hr/>
                            </th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr align="center">
                            <td align="left">Weight fraction of dry substances,%, not less than </td>
                            <td>40 &#x00B1;0.5</td>
                            <td>38 &#x00B1;0.5</td>
                        </tr>
                        <tr align="center">
                            <td align="left">Acidity, &#x00B0;T, no more than</td>
                            <td>20</td>
                            <td>20</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
        </sec>
        <sec sec-type="conclusion">
            <title>CONCLUSION</title>
            <p>The positive effect of melon concentrates on the process of structure formation of whey is scientifically substantiated and practically proved.</p>
            <p>Studies have found that to ensure optimal conditions of structure formation in a mixture of whey and melon concentrates, a dose of a mixture of gelling agents in an amount of 0.025% of the total weight is required.</p>
            <p>To optimize the technological parameters in the direction of ensuring maximum yield of jelly, it is advisable to partially replace carrageenan with carob gum, which in this combination shows synergistic properties.</p>
        </sec>
    </body>
    <back>
        <ack>
            <title>Acknowledgments:</title>
            <p>The work was supported by the Russian Foundation for Basic Research, project no. 15-16-10000, SSI NIIMMP “Development and scientific substantiation of new approaches to the production of livestock raw materials and increasing the biological value of socially important products based on modern biotechnological and molecular genetic methods”. Grant sponsors were not directly involved in the development, analysis, or writing of this article.</p>
        </ack>
        <ref-list>
            <ref id="b1">
                <element-citation publication-type="journal">
                    <person-group person-group-type="author">
                        <name name-style="western">
                            <surname>Ajewole</surname>
                            <given-names>I.A.</given-names>
                        </name>
                        <name name-style="western">
                            <surname>Ozo</surname>
                            <given-names>O.N.</given-names>
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